Peroxidase and lipid peroxidation of soybean roots in response to p-coumaric and p-hydroxybenzoic acids

Abstract

The scope of the present study was to investigate how the p-coumaric (p-CA) and p-hydroxybenzoic (p-HD) acids affect the peroxidase (POD, EC 1.11.1.7) activity, the lipid peroxidation (LP) and the root growth of soybean (Glycine max (L.) Merr.). Three-day-old seedlings were cultivated in nutrient solution containing p-CA or p-HD (0.1 to 1 mM) for 48 h. After uptake, both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW

Caffeic acid inhibits in vitro rooting in mung bean [Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress

“…Hydrogen peroxide accumulation further enhances lipid peroxidation and results in the disruption of cell metabolism, leading to cell death (Stone and Yang 2006). A number of studies have indicated that phenolic acids, including CA, affect membrane permeability and thus affect plant growth (Baziramakenga et al 1995;Doblinski et al 2003;Ju et al 2007). Baziramakenga et al (1995) demonstrated that benzoic and cinnamic acids induced ROS generation, which severely affected the integrity of the membrane.…”

“…Baziramakenga et al (1995) demonstrated that benzoic and cinnamic acids induced ROS generation, which severely affected the integrity of the membrane. Doblinski et al (2003) reported that p-coumaric and p-hydroxybenzoic acids (0.5-1.0 mM) caused lipid peroxidation in soybean roots, resulting in growth inhibition. Caffeic acid is also known to alter root activity by depolarizing the root membrane (Glass and Dunlop 1974); however, the mechanism by which this occurs involves ROS is not still clear.…”

Naringenin inhibits the growth and stimulates the lignification of soybean root

“…Degradation of flavanones in benzoic acid and their derivatives has been previously reported (Barz and Hoesel, 1979), and the non-enzymatic cleavage of naringenin produces p-hydroxybenzoic acid (Newhall and Ting, 1967). The potential of this phenolic compound to be allelopathic has been widely reported (Politycka, 1996;Doblinski et al, 2003;Inderjit and Duke, 2003;Weir et al, 2004). In agreement with this, enhanced quercetin autooxidation leads to the formation of the 3,4-dihydroxybenzoic acid (Takahama and Hirota, 2000).…”

The effects of dopamine on antioxidant enzymes activities and reactive oxygen species levels in soybean roots

“…The extract was centrifuged (10,000 £ g, 15 min, 4 C), and the supernatant was used to determine the content of MDA. 32 An aliquot of 0.5 ml of the supernatant was added to 4 ml of 0.5% of thiobarbituric acid (prepared in 20% of trichloroacetic acid). The mixture was heated in boiling water for 10 min and then quickly cooled in an ice-bath; samples were then centrifuged (3,500 £ g, 5 min).…”