Caffeic acid inhibits in vitro rooting in mung bean [Vigna radiata (L.) Wilczek] hypocotyls by inducing oxidative stress

Abstract

Caffeic acid (CA), which is ubiquitously present in plants, is a potent phytotoxin affecting plant growth and physiology. The aim of our study was to investigate whether CA-induced inhibition of adventitious root formation (ARF) in mung bean {Vigna radiata (L.) Wilczek [Phaseolus aureus Roxb.]} involves the induction of conventional stress responses. The effect of CA (0-1000 lM) on ARF in mung bean was determined by measuring the generation of reactive oxygen species (ROS) in terms of malondialdehyde and hydrogen peroxide (H 2 O 2 ) content, root oxidizability and changes in levels of antioxidant enzymes. Our results show that CA significantly enhanced MDA content, indicating severe lipid peroxidation, and increased H 2 O 2 accumulation and root oxidizability in the lower rooted hypocotylar region (LRHR) of mung bean, thereby inducing oxidative stress and cellular damage. In response to CA, there was a significant upregulation in the activities of scavenging enzymes, such as superoxide dismutase, ascorbate peroxidase, guaiacol peroxidase, catalase and glutathione reductase, in LRHRs of mung bean. Based on these results, we conclude that CA inhibits ARF in mung bean hypocotyls by inducing ROS-generated oxidative stress and upregulating the activities of antioxidant enzymes.

Ferulic acid impairs rhizogenesis and root growth, and alters associated biochemical changes in mung bean (Vigna radiata) hypocotyls

“…Moreover, PODs activity increases significantly in response to allelochemicals, including phenolic acids (Baziramakenga et al 1995;Weir et al 2004). Singh et al (2009) demonstrated that caffeic acid inhibits root growth through generation of reactive oxygen species (ROS) in Phaseolus aureus. Baziramakenga et al (1995) demonstrated that phenolic acids undergo oxidation to form quinines that are highly reactive free radicals.…”

“…Nevertheless, phenolic acids interfere with the activity of plant growth regulators like auxins and cytokinins (Einhellig 2004). Further, we did not explore whether the observed reduction in the rooting of mung bean hypocotyls involve any induction of oxidative stress through ROS generation, since phenolics have been reported to affect plant growth through oxidative stress induction (Weir et al 2004;Oracz et al 2007;Singh et al 2009). …”

Cinnamic acid pretreatment mitigates chilling stress of cucumber leaves through altering antioxidant enzyme activity

“…For example, heat pretreatment mitigates chilling stress of plants through regulating antioxidant activities (Zhang et al, 2005); pre-acclimation to drought stress (Khanna-Chopra and Selote, 2007) and cold conditions (Tao et al, 1998) influences antioxidant activities in relation to stress resistance in plants. Salicylic acid, caffeic acid and other natural or synthetic molecules can create oxidative stress in plant cells and tissues (Ganesan and Thomas, 2001;Singh et al, 2009). However, when applied exogenously at suitable concentrations, salicylic acid enhances the efficiency of the antioxidant system and alleviates chilling stress of seedlings (Hayat et al, 2010).…”

Chemical composition of Clinopodium menthifolium aqueous extract and its influence on antioxidant system in black nightshade (Solanum nigrum) and pepper (Capsicum annuum) seedlings and mortality rate of whitefly (Trialeurodes vaporariorum) adults

“…Due to the exposure to caffeic acid, an increase of the lipid peroxidation process was observed in the root of bean [23] and cabbage [24]. In this research, C. menthifolium aqueous extract which contains caffeic acid at a concentration of 13.9 ± 0.7µg/g, induced lipid peroxidation in black nightshade leaves and roots.…”