Peroxidase Activity and Lignification in the Pod Membrane of Pisum Sativum L.

Bowling, Alison C; Crowden, RK

doi:10.1071/bi9730679

Cited by 9 publications

(2 citation statements)

References 8 publications

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“…It may enhance oxidation of phenolic compounds to products toxic to the infecting agent or to the infected cells (2,14,24); in the latter case, necrotic foci prevent the spread of the infectious agent (11,19,20,25,26). It may increase the lignification of cell walls, rendering them impervious to degradation or penetration by the pathogen, or both (6,8,15).…”

Section: Discussionmentioning

confidence: 99%

Cell Wall and Protoplast Isoperoxidases of Corn Leaves in Relation to Cut Injury and Infection with Helminthosporium maydis

1975

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The nature of the involvement of peroxidases in plant resistance to fungal, bacterial, and viral diseases has been extensively investigated (7,13,18,22,28,30). However, the emphasis has been on soluble peroxidases, separated from tissue homogenates by centrifugation, and the cell wall-bound components have usually been disregarded. Since parasitic microorganisms are in intimate association with host cell walls at entry, at the establishment of the host-parasite relationship, and during spread in the host tissues, cell wall peroxidase fractions may be specifically involved in the plant-response to the parasite.In previously reported research on tobacco pith and sweet potato root parenchyma (5), significant differences were found between cell wall and protoplast peroxidases in their isoenzyme patterns, activity, and reaction to mechanical injury. It has been University, Columbus, Ohio 43210shown recently that the reaction of tobacco leaf peroxidase to cutting injury did not differ from that to tobacco mosaic virus infection in respect to the qualitative isoperoxidase patterns in the protoplast and cell wall-free or ionically and covalently bound fractions (4).This report deals with the reaction of corn leaf peroxidase to cutting injury and to infection with the fungus Helminthosporium maydis. Funk inbred lines with normal and Texas male-sterile cytoplasm were used. In a previous investigation (10), the Tms' cytoplasm inbred showed greater susceptibility to the fungus than did the N cytoplasm inbred; this was also reflected in a greater electrolyte and peroxidase leakage from the infected leaves. MATERIALS AND METHODSThe plants were grown in vermiculite on a 4-fold diluted Hoagland's solution in a growth chamber at 28 C and 16-hr daily photoperiod (1200 ft-c). At the four leaf stage, the laminae of the second and third leaves were detached and immediately weighed, stapled to a sheet of Whatman No. 3 filter paper, and cut into 8-mm segments or sprayed with a H. maydis race T spore suspension (20,000-30,000/ml) in H,O containing 0.1 % Tween 40. The suspension was prepared from cultures incubated on a glucose-L-asparagine agar medium for 12 to 14 days at 28 C. Intact (control) and cut laminae were sprayed with water containing 0.1% Tween 40. Six laminae from three plants were used in each of three replications per treatment. The laminae were incubated in the dark in a water vaporsaturated atmosphere for 16 and 40 hr. At the end of incubation, the infected laminae of the N and Tms cytoplasm inbreds had oblong lesions 1 to 2 and 3 to 4 mm long, respectively. The Tms inbred showed also slight chlorosis and some flaccidity.Immediately after sampling, the tissue was frozen and kept in a deep freeze. Unfortunately, attempts to isolate free peroxidase from cell walls before freezing by vacuum-infiltration, followed by centrifugation, were not successful. Therefore, the protoplast peroxidase fraction was separated together with the free fraction in the cell wall.Procedures related to peroxidase isolation were similar ...

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Section: Discussionmentioning

confidence: 99%

Cell Wall and Protoplast Isoperoxidases of Corn Leaves in Relation to Cut Injury and Infection with Helminthosporium maydis

1975

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“…Using gel electro phoresis, Conkle (1971) found that peroxidase isozymes were absent in stratified seeds of Pinus attenuata, but increased in numbers and stainability after radicle emergence. Bowling and Crowden (1973) suggested that peroxidase may pre dispose cells to lignification without being directly involved in the lignification process per se. Peroxidase in plant tissues consists of a number of isozymes with peroxidase activity, raising the possibility that there may be a division of function of peroxidases ^ vivo (Mills and Crowden, 1968).…”

Section: The Abundance Of Lipid Bodies Can Present a Problem To The Lmentioning

confidence: 99%

An ultrastructural and microspectrophotometric study of leaf initiation in germinating Pinus banksiana seeds

Cecich

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Gymnosperm trees are among the most economically important plants in the world, supplying raw products for the construction, paper and chemical industries. Most anatomical research, therefore, has been directed toward the cambium, the lateral meristem which produces the secondary xylem or wood. There has been a limited amount of research done on the shoot apical meristem of mature trees. This literature has been reviewed elsewhere and will not be covered here (Korody, 1937;

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Studies on peroxidases and vascular discoloration in cassava root tissue

1981

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Extracts from discoloured cassava root tissue contained peroxidase activity in the soluble, covalently‐bound and ionically‐bound fractions. Extracts from non‐discoloured tissue lacked activity in the latter fraction and showed reduced activity in the other two fractions. Fractions from discoloured tissue showed nine soluble, two ionically‐bound and three covalently‐bound activity bands. Eight soluble peroxidase bands and one covalently‐bound band were detected by electrophoresis in fractions from non‐discoloured tissue. Some bands were less intensely stained than the corresponding bands detected in discoloured tissue extracts. Polyphenol oxidase showed increased activity only in the covalently‐bound fraction from discoloured tissue. Cassava roots produced ethylene in response to mechanical damage. Production commenced 6 h after injury and was only slightly affected by the accumulation of carbon dioxide. Neither the presence of carbon dioxide nor of exogenous ethylene had much effect on the electrophoretic pattern of soluble peroxidases.

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Peroxidase Activity and Lignification in the Pod Membrane of Pisum Sativum L.

Cited by 9 publications

References 8 publications

Cell Wall and Protoplast Isoperoxidases of Corn Leaves in Relation to Cut Injury and Infection with Helminthosporium maydis

Cell Wall and Protoplast Isoperoxidases of Corn Leaves in Relation to Cut Injury and Infection with Helminthosporium maydis

An ultrastructural and microspectrophotometric study of leaf initiation in germinating Pinus banksiana seeds

Studies on peroxidases and vascular discoloration in cassava root tissue

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