Permethrin enhances the agonist activity of dinotefuran on insect cholinergic synaptic transmission and isolated neurons

Abstract

Insect resistance mechanisms against pesticides lead to the development and the search of new pesticide combinations in order to delay the resistance. The combination of neonicotinoids with pyrethroids was currently proposed but the mode of action of these compounds at synaptic and extrasynaptic levels needs to be further explored. In the present study, we evaluated the effect of the combination of two insecticides, permethrin and dinotefuran, on cockroach cholinergic synaptic transmission and on isolated cell bodies. We first found that combination of 5 μM permethrin and dinotefuran enhances depolarization of the sixth abdominal ganglion compared to dinotefuran alone, without an inhibition of the spontaneous activity. However, a pretreatment with 1 μM dinotefuran or permethrin before bath application of the mixture inhibits the ganglionic depolarization. Compared to permethrin, 1 μM dinotefuran induces a persistent enhancement of spontaneous activity. Interestingly, at extrasynaptic level, using dorsal unpaired median neurons and Kenyon cells, we found that combination of both 1 μM dinotefuran and permethrin resulted in an increase of the mixture-induced current amplitudes. Pretreatment with 1 μM dinotefuran strongly decreases the currents whereas permethrin induces a time-dependent inhibition. These data demonstrate that the combination of dinotefuran and permethrin enhances the effect of dinotefuran.

Enhanced fungicidal efficacy and improved interfacial properties with the co‐delivery of prothioconazole and tebuconazole using polylactic acid microspheres

“…With the proliferation of research, pesticide compounding technology has become a principal tool for the improvement of utilization rate, the reduction of dosage and the enhancement of pesticide efficacy. [16][17][18][19] Traditional pesticide compounding technology and nano/micrometer drug delivery systems can be combined to form co-delivery systems, which can effectively improve the utilization rate of a pesticide. Chen et al used a metal-organic skeleton (NH2-Al-MIL-101) as a carrier and simultaneously loaded Azoxil (AZOX) and tenazolol (Dini) to obtain an AZOX@Dini@NH2-Al-MIL-101 dual-loaded system, showing that the combination of fungicides with different action modes had a potential application prospect for plant protection.…”

“…With the proliferation of research, pesticide compounding technology has become a principal tool for the improvement of utilization rate, the reduction of dosage and the enhancement of pesticide efficacy 16–19 . Traditional pesticide compounding technology and nano/micrometer drug delivery systems can be combined to form co‐delivery systems, which can effectively improve the utilization rate of a pesticide.…”

Effect of Dinotefuran, Permethrin, and Pyriproxyfen (Vectra® 3D) on the Foraging and Blood-Feeding Behaviors of Aedes albopictus Using Laboratory Rodent Model

“…DPP compounds are contact poisons and do not require ingestion by the insect to be effective [ 17 ]. Dinotefuran and Permethrin act as synergetic compounds inducing continuous nerve stimulation, incoordination, tremors, and death of the insect [ 17 , 18 ]. While the third compound (Pyriproxyfen) acts as a suppressor of embryogenesis of the insect [ 18 ].…”