Permeación en piel humana de una nanoemulsión de ftalocianina de aluminio clorada para la optimización de tratamientos tópicos de leishmaniasis cutánea

Ospina, Victoria; Mantilla, Julio César; Conde, Carlos Arturo; Escobar, Patricia

doi:10.12804/revsalud12.2.2014.05

Cited by 5 publications

(3 citation statements)

References 38 publications

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“…Same results have been reported in dorsal skin of Wistar rats and abdominal skin of human. In these studies, the photosensitizers did not permeate the skin but were retained by the stratum corneum [ 24 33 ]. Conversely, significant amounts of PM were found in the spleen and in the liver of both irradiated and non-irradiated mice, treated with topical PM-loaded NE.…”

Section: Discussionmentioning

confidence: 99%

Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis

et al. 2021

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Background Photodynamic therapy (PDT) using chloroaluminium phthalocyanine (ClAlPc) and paromomycin sulfate (PM) can be effective against New World Leishmania species involved in cutaneous leishmaniasis (CL). The aim of this study is to assay the skin permeation and the antileishmanial effects of a nanoemulsion (NE) containing both ClAlPc and PM in experimental CL by Leishmania (Viannia) braziliensis . Material and Methods Cremophor ELP/castor oil-based NEs were prepared by a low-energy method and characterized for their physicochemical parameters. The NEs were used to deliver both ClAlPc and PM to leishmania cells. The in vitro toxicity of NEs were tested in vitro against L. (V.) braziliensis and THP-1 cells. The in vivo toxicity was assessed in non-infected BALB/c mice. Ex-vivo permeation and retention studies using healthy mice skin were also conducted. Finally, the in vivo activity of NE-PM+ClAlPc after PDT was tested in BALB/c mice infected with parasites. Results NEs are colloidally stable with average droplet diameter of 30 nm, polydispersity index (PDI) below 0.2, and zeta potential near zero. Both promastigotes and intracellular amastigotes treated with NE-PM, NE-ClAlPc and NE-PM+ClAlPc were inhibited at >50%, >95%, >88%, respectively, after PDT with a phototoxic index (PI) >1.2. No skin ClAlPc permeation was observed. In contrast, PM skin permeation was 80-fold higher using PM-loaded NE formulation in comparison to aqueous PM solution. Topical treatment with NE formulations showed no signs of local toxicity or genotoxicity. In addition, concentrations of PM between 27.3 - 292.5 μM/25 mg of tissue were detected in different organs. In vivo , the NE-PM+ClAlPc treatment did not reduce skin lesions. Conclusion The Cremophor ELP/castor oil NE formulation increases the permeation of PM through the skin and can be used to co-deliver PM plus ClAlPc for combined PDT protocols. However, the lack of efficacy in the in vivo model evidences that the therapeutical scheme has to be improved.

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Section: Discussionmentioning

confidence: 99%

Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis

et al. 2021

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“…Com relação ao tratamento, o meio mais utilizado até hoje é o tratamento sistêmico, com o antimonial pentavalente, seguidos de outros fármacos como anfotericina B lipossomal, isetionato de pentamidina (OSPINA et al, 2014).…”

Section: Introductionunclassified

“…Doses altas das medicações sistêmicas são responsáveis por diversos efeitos tóxicos, casos de resistências e co-infecção. Sendo assim, estudos recentes, vem trazendo opções de tratamentos tópicos para facilitar a adesão e contribuir com os esquemas terapêuticos (OSPINA et al, 2014).…”

Section: Introductionunclassified

Leishmaniose Cutânea E Seus Novos Levantamentos Sobre Perfis Epidemiológicos Dos Reservatórios E Tratamentos Tópicos: Relato De Caso / Leishmaniose Cutânea and Its New Surveys on Epidemiological Profiles of Reservoirs and Topical Treatments: Case Report

Magalhães¹,

Nascimento²,

Rezende³

et al. 2020

BJD

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Optimization of extraction method and evaluation of antileishmanial activity of oil and nanoemulsions of Pterodon pubescens benth. fruit extracts

Santos

Garcia

Outuki

et al. 2016

Experimental Parasitology

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Permeación en piel humana de una nanoemulsión de ftalocianina de aluminio clorada para la optimización de tratamientos tópicos de leishmaniasis cutánea

Cited by 5 publications

References 38 publications

Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis

Nanoemulsions with Chloroaluminium Phthalocyanine and Paromomycin for Combined Photodynamic and Antibiotic Therapy for Cutaneous Leishmaniasis

Leishmaniose Cutânea E Seus Novos Levantamentos Sobre Perfis Epidemiológicos Dos Reservatórios E Tratamentos Tópicos: Relato De Caso / Leishmaniose Cutânea and Its New Surveys on Epidemiological Profiles of Reservoirs and Topical Treatments: Case Report

Optimization of extraction method and evaluation of antileishmanial activity of oil and nanoemulsions of Pterodon pubescens benth. fruit extracts

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