Permeability and mechanical properties of gradient porous PDMS scaffolds fabricated by 3D-printed sacrificial templates designed with minimal surfaces

Montazerian, Hossein; Mohamed, Manal G.; Montazeri, Mahbobeh; Kheiri, Sina; Milani, Abbas S.; Kim, Keekyoung

doi:10.1016/j.actbio.2019.06.040

Cited by 161 publications

(121 citation statements)

References 58 publications

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“…Additionally, the flexibility of operation allows the manufacturing of individual batches of scaffolds personalized to a specific defect obtained from a patient by X-ray computed tomography (CT) or magnetic resonance imaging (MRI) techniques [25]. Using this Computer-Aided Tissue Engineering technique, unique functional scaffold pieces of personalized shape can be printed (Figure 2c), addressing osteochondral defects or designing scaffolds for complex-shaped human organs [26,31,32]. This technique plays an important role in the manufacturing of porous tissue scaffolds for the regeneration of tissues with an appropriate shape and size so that cells can penetrate on it when nutrients are provided.…”

Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

“…In addition, the rough surfaces obtained, the necessity of a support in some cases that should be removed, and the limited horizontal (100-150 µm) and vertical resolution (ca. 100 µm minimum layer thickness) of FDM printers in comparison with other 3D-printing methods are other disadvantages of this process [22,26,27,34]. Namely, FDM resolution is limited by the raw material properties, equipment specifications, and dimensions of extruded filaments [21,24,35].…”

Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

“…Porous scaffolds designed on minimal surface architectures and fabricated through a mold printing approach with an FDM 3D printer can stimulate regenerative wound healing and contribute to tissue mechanical properties as well. Nowadays, both facts drive interest in scaffolds for tissue regenerative applications such as the treatment of large cutaneous defects [26,27]. For example, 3D poly-(esterurethane) (PUR) scaffolds were fabricated with tunable substrate modulus (5, 24, and 266 MPa) (Figure 2d-f).…”

Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

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Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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The regenerative medicine field is seeking novel strategies for the production of synthetic scaffolds that are able to promote the in vivo regeneration of a fully functional tissue. The choices of the scaffold formulation and the manufacturing method are crucial to determine the rate of success of the graft for the intended tissue regeneration process. On one hand, the incorporation of bioactive compounds such as growth factors and drugs in the scaffolds can efficiently guide and promote the spreading, differentiation, growth, and proliferation of cells as well as alleviate post-surgical complications such as foreign body responses and infections. On the other hand, the manufacturing method will determine the feasible morphological properties of the scaffolds and, in certain cases, it can compromise their biocompatibility. In the case of medicated scaffolds, the manufacturing method has also a key effect in the incorporation yield and retained activity of the loaded bioactive agents. In this work, solvent-free methods for scaffolds production, i.e., technological approaches leading to the processing of the porous material with no use of solvents, are presented as advantageous solutions for the processing of medicated scaffolds in terms of efficiency and versatility. The principles of these solvent-free technologies (melt molding, 3D printing by fused deposition modeling, sintering of solid microspheres, gas foaming, and compressed CO2 and supercritical CO2-assisted foaming), a critical discussion of advantages and limitations, as well as selected examples for regenerative medicine purposes are herein presented.

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Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

Section: D-printing By Fused Deposition Modeling (Fdm)mentioning

confidence: 99%

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Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

2020

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“…Regarding mechanical properties, a PDMS/thiol based photopolymer was developed for SLA with printed structures showing high elongation at break of up to 138%, and Young's modulus of 0.4-1.7 MPa [199]. Radially gradient pore distributions in scaffolds with pore sizes of 1 mm showed higher elastic modulus with an increase from 52 to 1038 kPa and higher fluid permeability [200] compared to scaffolds without pore gradients. Young's modulus can be also tuned from 48 to 1783 kPa by varying the ratio of prepolymer to curing agent from 50:1 to 10:1 respectively [201].…”

Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

“…Feature size 1 mm (SLA) [150]; Feature size 20 µm (TPP) [61] Human fetal osteoblasts [145]; human bone-marrow stromal cells [143]; Schwann cells [61]; osteoconductivity [147,158]; bacterial cell colonies [142] PEEK (4. Feature size 25 µm (SLA) [173]; Feature size 5 µm (TPP) [177][178][179]; Inclusion of silk and melanin, storage E: 1-2.5 kPa [134] Anti-bacterial [183]; hMSCs [174] PDMS (4.2.2) Feature size 250 µm [194] Feature size 250 µm (SLA) [195]; Feature size <1 µm (TPP) [196]; E: 0.4-1.7 MPa [199]; E: 0.05-1 MPa [200] Anti-bacterial [47]…”

Section: Sls (31) Fdm (32) Extrusion Bioprinting (33) Light-assistmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

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The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

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Preparation and Properties of Self‐Setting Calcium Phosphate Scaffolds: Effect of Pore Architecture

et al. 2023

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Self‐setting calcium phosphate cement (CPC) scaffold with interconnected macropores is hard to prepare without compromising its hydration reaction. Herein, an indirect 3D printing method is using to prepare CPC scaffolds. Detailedly, polycaprolactone (PCL)‐sacrificed models with different strut sizes are first printed by 3D plotting technique, and then the CPC pastes are perfused into the PCL models and then self‐setting. After the removal of the PCL models, the CPC scaffolds with different pore sizes are obtained. It is showed in the results that the prepared CPC scaffolds had uniform shape and 3D interconnected macropore structure. Meanwhile, the compressive strength of CPC scaffolds all meets the requirements of repairing cancellous bone. More interestingly, the CPC scaffold with about 300 μm in macropore size is more conducive to the ingrowth and osteogenesis differentiation of mouse bone marrow mesenchymal stem cells. As a whole, the indirect 3D printing method is a promising strategy for preparing CPC scaffolds, which are hard to form directly by 3D printing technique.

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Permeability and mechanical properties of gradient porous PDMS scaffolds fabricated by 3D-printed sacrificial templates designed with minimal surfaces

Cited by 161 publications

References 58 publications

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Solvent-Free Approaches for the Processing of Scaffolds in Regenerative Medicine

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

Preparation and Properties of Self‐Setting Calcium Phosphate Scaffolds: Effect of Pore Architecture

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