Permanent 3D microstructures in a polymeric host created using holographic optical tweezers

Jordan, Pamela; Clare, H.; Flendrig, Leonard M.; Leach, Joshua D.G.; Cooper, Jonathan M.; Padgett, Miles J.

doi:10.1080/09500340310001625768

Cited by 11 publications

(12 citation statements)

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“…In addition to the intrinsic excitement of forming microscopic 3-D structures, we believe that such structures will have applications in measuring mechanical properties of materials, photonic and biological crystal growth, tissue engineering, permanent extended 3-D structures, and manipulation within microfluidic devices. Some of these latter applications are aided by the fact that 3-D structures formed within optical tweezers can be made permanent by using a gel solution in which to trap the objects which are then locked in place when the gel sets [16].…”

Section: Resultsmentioning

confidence: 99%

Assembly of 3-dimensional structures using programmable holographic optical tweezers

Sinclair¹,

Jordan²,

Courtial³

et al. 2004

Opt. Express

141

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The micromanipulation of objects into 3-dimensional geometries within holographic optical tweezers is carried out using modified Gerchberg-Saxton (GS) and direct binary search (DBS) algorithms to produce the hologram designs. The algorithms calculate sequences of phase holograms, which are implemented using a spatial light modulator, to reconfigure the geometries of optical traps in many planes simultaneously. The GS algorithm is able to calculate holograms quickly from the initial, intermediate and final trap positions. In contrast, the DBS algorithm is slower and therefore used to pre-calculate the holograms, which are then displayed in sequence. Assembly of objects in a variety of 3-D configurations is semi-automated, once the traps in their initial positions are loaded.

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Section: Resultsmentioning

confidence: 99%

Assembly of 3-dimensional structures using programmable holographic optical tweezers

Sinclair¹,

Jordan²,

Courtial³

et al. 2004

Opt. Express

141

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“…This is substantiated in recent works showing the complementary role of optical trapping with laser-initiated photopolymerization for the construction and gelling of permanent particle arrays from linear to crystal-like structures. 13,14 Aside from the ability to form predefined structures in 3D, however, a number of applications, including those of biological relevance, would significantly gain from the power of being able to arbitrarily adjust the relative positions of particle aggregates over a full volume and in a manner that satisfies real human response time.…”

mentioning

confidence: 99%

“…With the advent of spatial light modulators ͑SLM͒, rapid developments boosting the degree of control for optically trapped particles have been seen in two techniques based on diffractive optics ͑DO͒ 12,13 and the generalized phase contrast ͑GPC͒ method. 11,[15][16][17] Recently, we have shown the suitability of the GPC approach for direct and user-interactive manipulation of a colony of particles and living cells simultaneously trapped and manipulated in a two-dimensional plane with no surface contact.…”

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confidence: 99%

Four-dimensional optical manipulation of colloidal particles

Rodrigo

Daria

Glückstad

2005

Applied Physics Letters

109

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We transform a TEM 00 laser mode into multiple counterpropagating optical traps to achieve four-dimensional simultaneous manipulation of multiple particles. Efficient synthesis and dynamic control of the counterpropagating-beam traps is carried out via the generalized phase contrast method, and a spatial polarization-encoding scheme. Our experiments genuinely demonstrate real-time, interactive particle-position control for forming arbitrary volumetric constellations and complex three-dimensional trajectories of multiple particles. This opens up doors for cross-disciplinary cutting-edge research in various fields.

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“…This is the first time that permanent, living cell arrays of such complexity have been synthesized to our knowledge. Previously, holographic arrays of optical traps have been used to permanently arrange up to nine Escherichia coli in gelatin (34,35), but the viability of the bacteria was not demonstrated. The extraordinarily long trapping time required to fix the position of a cell in gelatin (;60 min) will adversely affect the viability.…”

Section: Introductionmentioning

confidence: 99%

Laser-Guided Assembly of Heterotypic Three-Dimensional Living Cell Microarrays

Akselrod

Timp

Mirsaidov

et al. 2006

Biophysical Journal

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We have assembled three-dimensional heterotypic networks of living cells in hydrogel without loss of viability using arrays of time-multiplexed, holographic optical traps. The hierarchical control of the cell positions is achieved with, to our knowledge, unprecedented submicron precision, resulting in arrays with an intercell separation <400 nm. In particular, we have assembled networks of Swiss 3T3 fibroblasts surrounded by a ring of bacteria. We have also demonstrated the ability to manipulate hundreds of Pseudomonas aeruginosa simultaneously into two- and three-dimensional arrays with a time-averaged power <2 mW per trap. This is the first time to our knowledge that living cell arrays of such complexity have been synthesized, and it represents a milestone in synthetic biology and tissue engineering.

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Permanent 3D microstructures in a polymeric host created using holographic optical tweezers

Cited by 11 publications

References 0 publications

Assembly of 3-dimensional structures using programmable holographic optical tweezers

Assembly of 3-dimensional structures using programmable holographic optical tweezers

Four-dimensional optical manipulation of colloidal particles

Laser-Guided Assembly of Heterotypic Three-Dimensional Living Cell Microarrays

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