Laser-Guided Assembly of Heterotypic Three-Dimensional Living Cell Microarrays

Akselrod, Gleb M.; Timp, Winston; Mirsaidov, Utkur; Zhao, Qian; Li, C.; Timp, Rolf; Timp, K.; Matsudaira, Paul; Timp, G.

doi:10.1529/biophysj.106.084079

Cited by 91 publications

(67 citation statements)

References 66 publications

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“…We know from the early work of Ashkin and Smith how colloidal particles can act as nonlinear media Smith et al, 1982͒: with optical forces pulling particles into bright field lines one can see how a modulation of the refractive index might occur in such systems. Optical binding is not only restricted to colloidal objects but it is also applicable to living cells ͑Buican et al, 1987;Metzger et al, 2005͒. Exploitation of optically assisted organization of cells is still at its infancy; however, the topic holds promise as the control of initial cell patterns dictates subsequent cell growth for tissue engineering, cell signaling studies, and other studies in synthetic biology ͑Akselrod et al, 2006͒. In the remainder of this review we discuss optical binding and provide an overview of the key experiments and theoretical approaches in this area. While optical trapping has been in existence for over 40 years, one must note that the field of optical binding is far from being mature or well developed: we elucidate upon the present results obtained and challenges this field faces as well as indicate some future approaches that will lead to new discoveries.…”

Section: Introductionmentioning

confidence: 99%

Colloquium: Gripped by light: Optical binding

2010

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͑Published 3 June 2010͒The light-matter interaction has been at the heart of major advances from the atomic scale right to the microscopic scale over the past four decades. Confinement by light, embodied by the area of optical trapping, has had a major influence across all of the natural sciences. However, an emergent and powerful topic within this field that has steadily merged but not gained much recognition is optical binding: the importance of exploring the optically mediated interaction between assembled objects that can cause attractive and repulsive forces and dramatically influence the way they assemble and organize themselves. This offers routes for colloidal self-assembly, crystallization, and organization of templates for biological and colloidal sciences. In this Colloquium, this emergent area is reviewed looking at the pioneering experiments in the field and the various theoretical approaches that aim to describe this behavior. The latest experimental studies in the field are reviewed and theoretical approaches are now beginning to converge to describe the binding behavior seen. Recent links between optical binding and nonlinearity are explored as well as future themes and challenges.

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Section: Introductionmentioning

confidence: 99%

Colloquium: Gripped by light: Optical binding

2010

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“…Holographic optical tweezers (HOT) has recently found its applications in life science where parallel single cells in an array can be manipulated and studied simultaneously [9,10]. In combination with microfluidic systems, HOT offers several new possibilities for flow measurement [11] and the ability to control the local environment of single cells in parallel [12,13].…”

Section: Introductionmentioning

confidence: 99%

The effect of external forces on discrete motion within holographic optical tweezers

Eriksson¹,

Keen²,

Leach³

et al. 2007

Opt. Express

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Holographic optical tweezers is a widely used technique to manipulate the individual positions of optically trapped micron-sized particles in a sample. The trap positions are changed by updating the holographic image displayed on a spatial light modulator. The updating process takes a finite time, resulting in a temporary decrease of the intensity, and thus the stiffness, of the optical trap. We have investigated this change in trap stiffness during the updating process by studying the motion of an optically trapped particle in a fluid flow. We found a highly nonlinear behavior of the change in trap stiffness vs. changes in step size. For step sizes up to approximately 300 nm the trap stiffness is decreasing. Above 300 nm the change in trap stiffness remains constant for all step sizes up to one particle radius. This information is crucial for optical force measurements using holographic optical tweezers.

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“…Both technologies were developed in parallel in the branch of optical manipulation, but only recently, they were combined into more powerful tandem system that immediately manifested itself as an extraordinary optical tool allowing fast and complex beam-shaping. 17,18 We now show how to utilize this assembly to enhance the speed of the fibre-based fluorescent imaging by two orders of magnitude.…”

Section: +++4+++ ++I+t++mentioning

confidence: 99%

Exploiting multimode waveguides for pure fibre based fluorescence imaging

Čižmár¹,

Dholakia

2013

SPIE Proceedings

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There has been an immense drive in modern microscopy towards miniaturisation and fibre based technology. This has been necessitated by the need to access hostile or difficult environments particulalrly in-situ and in-vivo. Strategies to date have included the use of specialist fibres and miniaturised scanning systems accompanied by ingenious microfabricated lenses. In parallel recent studies of randomized light fields and their holographic control opened up new ways for imaging. We present a novel approach for this field by utilising disordered light within a standard multimode optical fibre for minimally invasive lensless microscopy and optical mode conversion. We demonstrate scanning fluorescence microscopy at acquisition rates allowing observation of dynamic processes such as Brownian motion of mesoscopic particles. As the sample plane can be defined at any distance from the fibre facet, we eliminate the need for complex or elaborate focusing optics (e.g. miniaturized objectives, GRIN lenses) and instead reconfigure the system dynamically to image different axial planes. Furthermore, we show how such control can realise a new form of mode converter and generate various types of advanced light fields such as propagation-invariant beams and optical vortices. These may be useful for future fibre based implementations of super-resolution or light sheet microscopy. To the best of our knowledge, this technology represents the narrowest possible image guiding system based on light propagation.

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Laser-Guided Assembly of Heterotypic Three-Dimensional Living Cell Microarrays

Cited by 91 publications

References 66 publications

Colloquium: Gripped by light: Optical binding

Colloquium: Gripped by light: Optical binding

The effect of external forces on discrete motion within holographic optical tweezers

Exploiting multimode waveguides for pure fibre based fluorescence imaging

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