Perlecan expression influences the keratin 15‐positive cell population fate in the epidermis of aging skin

Santos, Morgan Dos; Michopoulou, Anna; André-Frei, V.; Boulesteix, Sophie; Guicher, Christine; Dayan, Guila; Whitelock, John M.; Damour, O.; Rousselle, Patricia

doi:10.18632/aging.100928

Cited by 29 publications

(27 citation statements)

References 71 publications

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“…Basal epidermal cells divide asymmetrically, generating a committed suprabasal cell and a proliferative basal cell, and during this process β1 integrin, but not β4 integrin, contributing to the spindle orientation . Perlecan regulates both the survival and the self‐renewal capacity of basal keratinocytes by controlling the bioavailability of perlecan‐binding soluble factors involved in epidermal morphogenesis . Our study revealed that HEI increased the basal localizations of β1 integrin, perlecan and heparan sulfate.…”

Section: Discussionmentioning

confidence: 66%

1‐(2‐Hydroxyethyl)‐2‐imidazolidinone, a heparanase and matrix metalloproteinase inhibitor, improves epidermal basement membrane structure and epidermal barrier function

Iriyama

Yamanishi

Kunizawa

et al. 2019

Experimental Dermatology

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Daily exposure to sunlight is known to affect the structure and function of the epidermal basement membrane (BM), as well as epidermal differentiation and epidermal barrier function. The aim of this study is to clarify whether the inhibition of BM‐degrading enzymes such as heparanase and matrix metalloproteinase 9 (MMP‐9) can improve the epidermal barrier function of facial skin, which is exposed to the sun on a daily basis. 1‐(2‐hydroxyethyl)‐2‐imidazolidinone (HEI) was synthesized as an inhibitor of both heparanase and MMP‐9. HEI inhibited not only the BM damage at the DEJ but also epidermal proliferation, differentiation, water contents and transepidermal water loss abnormalities resulting from ultraviolet B (UVB). This was determined in this study by the use of UVB‐induced human cultured skins as compared with the control without HEI. Moreover, topical application of HEI improved epidermal barrier function by increasing water content and decreasing transepidermal water loss in daily sun‐exposed facial skin as compared with non‐treated skins. These results suggest that the inhibition of both heparanase and MMP‐9 is an effective way to care for regularly sun‐exposed facial skin by protecting the BM from damage.

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Section: Discussionmentioning

confidence: 66%

1‐(2‐Hydroxyethyl)‐2‐imidazolidinone, a heparanase and matrix metalloproteinase inhibitor, improves epidermal basement membrane structure and epidermal barrier function

Iriyama

Yamanishi

Kunizawa

et al. 2019

Experimental Dermatology

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“…Moreover, perlecan is involved in regulating solute transport and mechanosensing in the osteocyte lacunar-canalicular system [62], and its protein core can withstand over 100 pN of tension, suggesting that perlecan could act as an elastic tether in the lacunar-canalicular system [63]. Collectively, these properties culminate in a multifaceted biological agent that has been implicated in diseases such as osteoarthritis [64], atherosclerosis [65,66], muscle hypertrophy [67] cancer progression [22,24,27,68,69], aging [70], and pulmonary hypertension [71]…”

Section: Form Follows Function: Overview Of the Perlecan Gene And Promentioning

confidence: 99%

A current view of perlecan in physiology and pathology: A mosaic of functions

2017

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Perlecan, a large basement membrane heparan sulfate proteoglycan, is expressed in a wide array of tissues where it regulates diverse cellular processes including bone formation, inflammation, cardiac development, and angiogenesis. Here we provide a contemporary review germane to the biology of perlecan encompassing its genetic regulation as well as an analysis of its modular protein structure as it pertains to function. As perlecan signaling from the extracellular matrix converges on master regulators of autophagy, including AMPK and mTOR, via a specific interaction with vascular endothelial growth factor receptor 2, we specifically focus on the mechanism of action of perlecan in autophagy and angiogenesis and contrast the role of endorepellin, the C-terminal fragment of perlecan, in these cellular and morphogenic events.

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“…Perlecan gets a stabilizing function for basement membrane,4 by its interaction with type IV collagen and nidogen 1 and 2, but seems to be important also for wound healing at the functional level by the control of signaling molecules5,6 and stem cell niche 7…”

Section: Introductionmentioning

confidence: 99%

Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties

Chajra¹,

Auriol²,

Joly³

et al. 2016

CCID

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BackgroundThe aim of this study was to demonstrate that a defined cosmetic composition is able to induce an increase in the production of sulfated glycosaminoglycans (sGAGs) and/or proteoglycans and finally to demonstrate that the composition, through its combined action of enzyme production and synthesis of macromolecules, modulates organization and skin surface aspect with a benefit in antiaging applications.Materials and methodsGene expression was studied by quantitative reverse transcription polymerase chain reaction using normal human dermal fibroblasts isolated from a 45-year-old donor skin dermis. De novo synthesis of sGAGs and proteoglycans was determined using Blyscan™ assay and/or immunohistochemical techniques. These studies were performed on normal human dermal fibroblasts (41- and 62-year-old donors) and on human skin explants. Dermis organization was studied either ex vivo on skin explants using bi-photon microscopy and transmission electron microscopy or directly in vivo on human volunteers by ultrasound technique. Skin surface modification was investigated in vivo using silicone replicas coupled with macrophotography, and the mechanical properties of the skin were studied using Cutometer.ResultsIt was first shown that mRNA expression of several genes involved in the synthesis pathway of sGAG was stimulated. An increase in the de novo synthesis of sGAGs was shown at the cellular level despite the age of cells, and this phenomenon was clearly related to the previously observed stimulation of mRNA expression of genes. An increase in the expression of the corresponding core protein of decorin, perlecan, and versican and a stimulation of their respective sGAGs, such as chondroitin sulfate and heparan sulfate, were found on skin explants. The biosynthesis of macromolecules seems to be correlated at the microscopic level to a better organization and quality of the dermis, with collagen fibrils having homogenous diameters. The dermis seems to be compacted as observed on images obtained by two-photon microscopy and ultrasound imaging. At the macroscopic level, this dermis organization shows a smoothed profile similar to a younger skin, with improved mechanical properties such as firmess.ConclusionThe obtained results demonstrate that the defined cosmetic composition induces the synthesis of sGAGs and proteoglycans, which contributes to the overall dermal reorganization. This activity in the dermis in turn impacts the surface and mechanical properties of the skin.

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Perlecan expression influences the keratin 15‐positive cell population fate in the epidermis of aging skin

Cited by 29 publications

References 71 publications

1‐(2‐Hydroxyethyl)‐2‐imidazolidinone, a heparanase and matrix metalloproteinase inhibitor, improves epidermal basement membrane structure and epidermal barrier function

1‐(2‐Hydroxyethyl)‐2‐imidazolidinone, a heparanase and matrix metalloproteinase inhibitor, improves epidermal basement membrane structure and epidermal barrier function

A current view of perlecan in physiology and pathology: A mosaic of functions

Reactivating the extracellular matrix synthesis of sulfated glycosaminoglycans and proteoglycans to improve the human skin aspect and its mechanical properties

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