Periurethral Injection of Sustained Release Basic Fibroblast Growth Factor Improves Sphincteric Contractility of the Rat Urethra Denervated by Botulinum-A Toxin

Takahashi, Satoru; Chen, Qin; Ogushi, Tetsuo; Fujimura, Tetsuya; Kumagai, Jimpei; Matsumoto, Shimpei; Hijikata, Shigeki; Tabata, Yasuhiko; Kitamura, Tadaichi

doi:10.1016/j.juro.2006.03.070

Cited by 33 publications

(36 citation statements)

References 18 publications

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“…intravesical bladder pressure at which urine leakage occurs due to increased abdominal pressure in the absence of a detrusor contraction (Lin et al, 2008)). The decline of sphincter, and striated muscle, function has motivated the development of myoblast implants to improve continence (Furuta et al, 2007), or the use of basic fibroblast growth factor to facilitate muscle cell generation (Takahashi et al, 2006).…”

Section: Muscles Of the Urethramentioning

confidence: 99%

The physiological function of lower urinary tract smooth muscle

Fry

Meng

Young

2010

Autonomic Neuroscience

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Section: Muscles Of the Urethramentioning

confidence: 99%

The physiological function of lower urinary tract smooth muscle

Fry

Meng

Young

2010

Autonomic Neuroscience

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“…As a result, some groups have adopted this technique without spinal cord transection. 21 Additional methods of measuring urethral resistance are urethral closure pressure 22,23 as well as the measurement of abdominal leak point pressure via a catheter in the rectum and measurement of RUPP via a catheter introduced into the urethral meatus. 7 Because of the distensibility of the rodent abdomen, abdominal leak point pressure is not likely to have the same degree of sensitivity to injury and dysfunction as LPP.…”

Section: Assessment Of Sui In Animal Modelsmentioning

confidence: 99%

Animal Models of Female Stress Urinary Incontinence

et al. 2008

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Purpose-Urinary incontinence affects 40% of women in the United States and stress urinary incontinence accounts for a large portion of affected patients. As defined by the International Continence Society, stress urinary incontinence is the involuntary leakage of urine upon effort, exertion, sneezing or coughing. Since the ultimate success of long-term management for any condition is based on an understanding of its pathophysiology, and because the pathophysiology of stress urinary incontinence is incompletely defined, animal models have recently been developed to better understand stress urinary incontinence and develop novel treatment alternatives.Materials and Methods-Several animal models for urethral dysfunction have emerged in the last few years, including those based on pathophysiological theories of urethral sphincter dysfunction that were designed to simulate maternal birth trauma. Other models have focused on the creation of a durable model of dysfunction for investigating novel treatments.Results-Since animals cannot express intent, these animal models have focused on measuring decreased urethral resistance. The most widely used methods are the sneeze test, the tilt table technique and the leak point pressure test. Newer techniques include abdominal leak point pressure, urethral pressure measurement and retrograde urethral perfusion pressure. In addition to the advantages and disadvantages of each technique, all methods measure the composite contribution to urethral resistance from smooth and striated muscle, urethral closure and connective tissue, although none measures intent.Conclusions-We critically reviewed the different models of stress urinary incontinence and urethral dysfunction as well as the different methods of measuring urethral resistance. Keywords urethra; urinary incontinence; stress; female; urodynamics; models; animal Urinary incontinence affects 40% of women in the United States. SUI, the involuntary leakage of urine upon effort, exertion, sneezing or coughing, accounts for a large portion of affected patients. More than 165,000 surgical procedures are performed for SUI in the United States. It is estimated that a third of the procedures that are performed to treat SUI are done in patients with recurrent disease. These currently performed procedures for SUI are based on compensatory and nonphysiological mechanisms. Since the ultimate success of the long-term management of any condition is based on an understanding of its pathophysiology, and because †Correspondence:

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“…[16,57,58] Recently, the reconstruction of muscles based on regenerative medicine using a variety of biomaterials, cells, and bioactive molecules has been intensively attempted by many researchers, and somewhat promising results have been demonstrated. [7][8][9][10][11][12][13][14][15][16][17][18] However, the reinnervation of regenerated muscles has not yet been profoundly considered, even though reinnervation is an essential requirement to maintain muscle functions. Therefore, the aim of this study was the preparation of a bioactive molecule-loaded matrix system which can induce effective neurogenic differentiation of progenitor cells/ stem cells around the muscles and thus allow a reinnervation of the muscles.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a variety of attempts based on regenerative medicine have been conducted for this purpose. Several investigators have reported that various progenitor cells or stem cells [7][8][9][10][11][12][13][14][15] and continuously released bioactive molecules [16][17][18] can enhance the regeneration of muscles and thus help to treat chronic degenerative diseases. However, the regeneration of muscles alone cannot be a fundamental cure of chronic degenerative diseases, because regenerated muscles with insufficient nerve connections subsequently lead to muscle atrophy.…”

Section: Introductionmentioning

confidence: 99%

Dual growth factor-immobilized microspheres for tissue reinnervation:in vitroand preliminaryin vivostudies

Kim

et al. 2015

Journal of Biomaterials Science, Polymer Edition

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Growth factors (GFs) (basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF))-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and GFs onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28 days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real-time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, β-tubulin, and MAP2) and Western blot (Nestin and β-tubulin) analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker - β-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e. muscles) leading to sufficient reinnervation.

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Periurethral Injection of Sustained Release Basic Fibroblast Growth Factor Improves Sphincteric Contractility of the Rat Urethra Denervated by Botulinum-A Toxin

Cited by 33 publications

References 18 publications

The physiological function of lower urinary tract smooth muscle

The physiological function of lower urinary tract smooth muscle

Animal Models of Female Stress Urinary Incontinence

Dual growth factor-immobilized microspheres for tissue reinnervation:in vitroand preliminaryin vivostudies

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