Periplasmic binding protein based biosensors 1. Preliminary study of maltose binding protein as sensing element for maltose biosensor

Zhou, L.Q.; Cass, Anthony E. G.

doi:10.1016/0956-5663(91)87010-9

Cited by 36 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the literature, the immobilization of binding proteins has been reported in only two articles. Zhou and Cass (1991) studied the immobilization of maltose binding protein (MBP) labeled with IAEDANS. MBP-IAEDANS was immobilized onto PCG by glutaraldehyde coupling, carbodiimide coupling, and diazonium coupling.…”

Section: Response and Recovery Timesmentioning

confidence: 99%

Genetically engineered binding proteins as biosensors for fermentation and cell culture

Tolosa²,

Simpson³

et al. 2003

Biotech & Bioengineering

View full text Add to dashboard Cite

The signal-transduction properties and the potential applications of two engineered binding proteins from E. coli were extensively studied. Both proteins have a single cysteine mutation in their polypeptide chains, which allow the introduction of an environmentally sensitive fluorophore: ANS for glucose-binding protein (GBP) and acrylodan for glutamine-binding protein (QBP). Both proteins respond to their ligands in the micromolar range. The proteins can be stored at 4 degrees C for at least 5 months. Apparent binding constant, protein concentration, and fluorophore are three major factors that affect the biosensor's responsive ranges. The binding of the ligand is quick and reversible in solution, but the unfavorable dissociation equilibrium and mass-transfer resistance for encapsulated proteins can delay the response to several minutes and the recovery to hours. Simulated results show that using dialysis tubing with a diameter of 1 mm or less is possible to reduce the recovery time to less than 30 minutes. The potential applications of GBP were studied in yeast fermentation and E. coli fermentations in three different scales: 150 mL, 5 mL, and 100 microL. The results were compared with an YSI 2700 Chemistry Analyzer. Although the latter could not give reliable results for the E. coli fermentations as the glucose concentration in LB medium is close to its lower detection limit, the glucose biosensor presented here was successfully applied to each situation. Glutamine-binding protein was tested in cell cultures of two different scales (100 mL and 100 microL) and the results were also compared with those obtained with YSI. Both QBP and YSI gave good results for the 100-mL cell culture, but the relatively large sample volume requirement of YSI (at least 5 microL) prevented it from being used in the 100-microL cell culture. Because of their small sample volume requirements (less than 1 microL) and high sensitivity, the assays described here might find wide applications in high-throughput bioprocessing.

show abstract

Section: Response and Recovery Timesmentioning

confidence: 99%

Genetically engineered binding proteins as biosensors for fermentation and cell culture

Tolosa²,

Simpson³

et al. 2003

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Protein based biosensors offer low costs and good selectivity suitable for operation in a wide array of life science applications such as environmental monitoring, food quality control and blood analysis for the measurement of specific molecules including bio-available heavy metals and maltose or glucose (Bontidean et al 1998;Zhou and Cass 1991;Yi Jae Dae et al 2008). Among these applications, blood glucose monitoring is very important for millions of diabetes patients.…”

Section: Introductionmentioning

confidence: 98%

Handheld impedance biosensor system using engineered proteinaceous receptors

Ghafar-Zadeh

Chowdhury

Aliakbar

et al. 2010

Biomed Microdevices

View full text Add to dashboard Cite

We put forward an impedometric protein-based biosensor platform suitable for point-of-care diagnostics. A hand-held scale impedance reader system is described for the detection of corresponding physiochemical changes as the immobilized proteins bind to the analyte molecules in the proximity of the microfabricated electrodes. Specifically, we study the viability of this approach for glucose biosensing purposes using genetically engineered glucokinase as receptor proteins. The proposed reagent-less biosensor offers a high sensitivity of 0.5 mM glucose concentration level in the physiologically relevant range of 0.5 mM to 7.5 mM with less than 10 s response time.

show abstract

“…The reducing sugars in starch hydrolysate are mainly glucose and maltose. Glucose sensors are already well developed, while maltose sensors, though having been described in some research papers, are not yet suitable for practical use (e. g. Cordonnier et al, 1975;Bertrand et al, 1981;Garton et al, 1989;Zhou et al, 1991;Kawakami et al, 1992;Varadit et al, 1993). Most of the reported maltose electrodes have combined the enzymatic hydrolysis of maltose to glucose with a glucose oxidase electrode.…”

Section: Introductionmentioning

confidence: 99%

Kinetic study on starch hydrolysis using a glucose/maltose sensing system

et al. 1995

View full text Add to dashboard Cite

A dual-enzyme electrode flow injection system that can simultaneously determine glucose and maltose is used for an on-line study of starch hydrolyses catalysed by amylases. With the working system, determinations can be made every 2 minutes. A 10 pL sample size with recycled back-flow minim&s any loss of the reaction medium. The production, growth and decay of glucose and maltose concentrations during starch hydrolysis under various enzymatic conditions can thus be closely monitored, making it useful for the study of the catalytic kinetics of amylases and in screening and analysing enzyme systems.

show abstract

Periplasmic binding protein based biosensors 1. Preliminary study of maltose binding protein as sensing element for maltose biosensor

Cited by 36 publications

References 16 publications

Genetically engineered binding proteins as biosensors for fermentation and cell culture

Genetically engineered binding proteins as biosensors for fermentation and cell culture

Handheld impedance biosensor system using engineered proteinaceous receptors

Kinetic study on starch hydrolysis using a glucose/maltose sensing system

Contact Info

Product

Resources

About