Periphytic biofilms accumulate manganese, intercepting its emigration from paddy soil

Geographic imprint and ecological functions of the abiotic component of periphytic biofilms

“…In spite of the quantitative importance of EPS in periphytic biofilms and the above correlation analyses notwithstanding, conclusive evidence for the significance of the EPS component in paddy periphytic biofilms is still lacking. Accumulation is one efficient way of periphytic biofilm in modulating nutrients/elements cycling in paddy fields [4, 6]. In the present study, we quantified the accumulation potential of C, N, and P in periphytic biofilm, and found that the EPS component shapes the functions of periphytic biofilm in the accumulation of the three nutrients.…”

“…Therefore, maybe certain climatic factors would lead to the decreased synthesis of EPS by periphytic biofilms, resulting in the difference in the EPS content in periphytic biofilms grown in different rice planting areas. In addition, it is well known that climatic factors, such as lighting and temperature, can affect the growth of microbes thus shifting the microbial community structure in periphytic biofilms [4,18]. As prokaryotes and eukaryotes potentially showed different roles in the EPS content in periphytic biofilms, thus the climate-induced difference in the structures of prokaryotes and eukaryotes in different periphytic biofilms (cf.…”

“…2500 platforms, respectively. The methods of DNA extraction and purification, sequencing, quality control, sequence analysis, sequences deposition into the National Center for Biotechnology Information (NCBI) database, and so forth, were as described previously [4]. Microbial sequences were then deposited in the NCBI under accession number PRJNA854262, and the Genome Warehouse in National Genomics Data Center [32,33], Beijing Institute of Genomics, Chinese Academy of Sciences/China National Center for Bioinformation, under accession number GWHBOSZ00000000.…”

“…The microbial component of periphytic biofilms has been relatively well studied [4,6], whereas the abiotic component has received less attention. Little is known about EPS content and composition of the periphytic biofilm, and about the potential ecological functions of this matrix.…”

“…Consisting of autotrophs and heterotrophs, these aggregates grow in both natural aquatic ecosystems (e.g., streams, wetlands, rivers, etc.) and constructed wetlands (e.g., paddy fields), where they play multiple, crucial roles in modulating element cycling [4]. Due to concerns about excessive fertilizer input in paddy fields, nitrogen (N) and phosphorus (P) management based on periphytic biofilm is receiving more and more attention [5].…”