Peripheral projections and neuropeptide coexistence in a subpopulation of fluoride-resistant acid phosphatase reactive spinal primary sensory neurons

Dalsgaard, C.‐J.; Ygge, Jan; Vincent, S.R.; Ohrling, Mikael; Dockray, Graham J.; Elde, Robert

doi:10.1016/0304-3940(84)90275-1

Cited by 51 publications

(17 citation statements)

References 17 publications

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“…In observation suggests, therefore, that SOM and NPY are the present study, labeling of perikarya in the DRG not involved in the sensory innervation of the ovary. after TB application to the surface of the ovary was Dalsgaard et al (1984) reported that FRAP, an enzyme prevented by transecting the ovarian artery and suspen-thought to function in neurotransmitter metabolism sory ligament. These results, therefore, confirm the (Nagy and Hunt, 1982), was present in both somatic and neural specificity of TB.…”

Section: Cell Diameter (Um)mentioning

confidence: 98%

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Neuropeptides in sensory perikarya projecting to the rat ovary

McNeill

Burden

1987

Am. J. Anat.

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Afferent perikarya in dorsal root ganglia (DRG) at the T13 and L1 segmental levels projecting to the rat ovary were identified by utilizing the fluorescent retrograde tracer true blue (TB). Subsequently, TB-labeled ovarian afferent perikarya in DRG were examined for vasoactive intestinal polypeptide (VIP), substance P (SP), cholecystokinin-8 (CCK-8), neuropeptide Y (NPY), and somatostatin (SOM) immunoreactivity and for the presence of fluoride-resistant acid phosphatase (FRAP) enzyme activity. Of the ovarian afferent perikarya at the T13 and L1 segmental levels, 20.5% displayed VIP immunoreactivity, 23.8% displayed SP immunoreactivity, and 43.1% were immunoreactive for CCK-8. No ovarian afferent perikarya contained SOM or NYP immunoreactivity or FRAP activity. It is suggested that different neuropeptides may participate in modulation of specific ovarian functions.

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Section: Cell Diameter (Um)mentioning

confidence: 98%

“…The role of NPY, SOM, and CCKS in ovarian function is not known. FRAP is found in subpopulations of DRG perikarya and is believed to play a role in neurotransmitter metabolism (Nagy and Hunt, 1982;Dalsgaard et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Neuropeptides in sensory perikarya projecting to the rat ovary

McNeill

Burden

1987

Am. J. Anat.

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“…Several lines of evidence suggest that SP is involved in signaling nociceptive information in the spinal cord. First, SP is contained primarily in small-diameter afferent fibers and is released in the spinal cord after a noxious stimulus (Hokfelt et al, 1975;Dalsgaard et al, 1984;Boehmer et al, 1989;Levine et al, 1993). Second, iontophoresis of SP onto the spinal cord has been reported to excite selectively the spinal nociceptive neurons with minimal effect on the non-nociceptive neurons (Radhakrishnan and Henry, 1991;Salter and Henry, 1991;however, see De Koninck and Henry, 1991).…”

Section: Abstract: Substance P Receptor; Tachykinin; Neurokinin-1; Nmentioning

confidence: 99%

Noxious Cutaneous Thermal Stimuli Induce a Graded Release of Endogenous Substance P in the Spinal Cord: Imaging Peptide ActionIn Vivo

et al. 1997

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Dorsal root ganglia (DRG) neurons synthesize and transport substance P (SP) to the spinal cord where it is released in response to intense noxious somatosensory stimuli. We have shown previously that SP release in vivo causes a rapid and reversible internalization of SP receptors (SPRs) in dorsal horn neurons, which may provide a pharmacologically specific image of neurons activated by SP. Here, we report that noxious heat (43°, 48°, and 55°C) and cold (10°, 0°, Ϫ10°, and Ϫ20°C) stimuli, but not innocuous warm (38°C) and cold (20°C) stimuli, applied to the hindpaw of anesthetized rats induce SPR internalization in spinal cord neurons that is graded with respect to the intensity of the thermal stimulus. Thus, with increasing stimulus intensities, both the total number of SPRϩ lamina I neurons showing SPR internalization and the number of internalized SPRϩ endosomes within each SPR immunoreactive neuron showed a significant increase. These data suggest that thermal stimuli induce a graded release of SP from primary afferent terminals and that agonist dependent receptor endocytosis provides evidence of a spatially and pharmacologically unique "neurochemical signature" after specific somatosensory stimuli. Key words: substance P receptor; tachykinin; neurokinin-1; nociception; pain; sensory neuronNeurons with cell bodies located in dorsal root ganglia (DRG) convey somatosensory information from peripheral tissues to the CNS. These DRG neurons synthesize several neurotransmitters that are released into the spinal cord to signal somatosensory stimulation. To understand f urther how DRG neurons convey somatosensory information and how somatosensory information is processed by spinal cord neurons, several issues need to be clarified. First, it remains to be determined whether release of a particular sensory neurotransmitter(s) in the spinal cord is correlated with stimulation of a particular sensory modality and with excitation of a specific group of primary afferent fibers. Second, it is important to determine whether transmitter release is graded with stimulus intensity. Last, correlation of the intensity of sensory stimulation with the neurochemical changes that take place in the spinal cord will begin to provide data on how somatosensory stimuli are encoded within the spinal cord and whether different intensities and modalities of sensory stimulation generate a unique "neurochemical signature" within the spinal cord.Substance P (SP), an undecapeptide that is present in 20 -30% of DRG neurons, is one of the most extensively studied primary afferent neurotransmitters. Several lines of evidence suggest that SP is involved in signaling nociceptive information in the spinal cord. First, SP is contained primarily in small-diameter afferent fibers and is released in the spinal cord after a noxious stimulus (Hokfelt et al., 1975;Dalsgaard et al., 1984;Boehmer et al., 1989;Levine et al., 1993). Second, iontophoresis of SP onto the spinal cord has been reported to excite selectively the spinal nociceptive neurons with minimal...

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“…Accordingly, FRAP is believed to be synthesized in small primary afferent neurons and conveyed to the central (Knyihar, 1971) and peripheral (Devor and Claman, 1980) terminals by axonal flow. Thirty to 50% of the DRG cells projecting to the splanchnic or intercostal nerve are FRAPpositive (Dalsgaard et al, 1984). Moreover, numerous unmyelinated axons innervating the corneal epithelium exhibit acid phosphtase activity (Knyihar-Csillik and Csillik, 1981).…”

mentioning

confidence: 99%

FRAP-positive and Capsaicin-Sensitive Terminals in the Substantia Gelatinosa of the Mouse Spinal Trigeminal Nucleus Caudalis

Hiura

Nasu

Ishizuka

1999

Okajimas Folia Anatomica Japonica

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Summary: FRAP (fluoride-resistant acid phosphatase) -reactivity in the substantia gelatinosa of the mouse spinal trigeminal nucleus caudalis (STNC) was examined by light and electron microscopy. Degenerated figures of terminals caused by capsaicin were compared with the FRAP-positive terminals. Scalloped (fan-like) or indented, sinuous, slender, and cap-like figures with closely packed agranular synaptic vesicles of various sizes were common to both FRAPpositive and capsaicin-sensitive terminals. These terminals had glomerular or nonglomerular endings. Sometimes FRAP-positive and capsaicin-sensitive glomerular terminals made presynapses with surrounding dendrites. Frequently, both nonglomerular terminals were in direct contact with the neuronal soma. The terminal features of FRAP-positive and capsaicin-sensitive ones in the mouse STNC are the same as those seen in the superficial dorsal horn of the spinal cord. These findings suggest that some of the FRAP-positive terminals are capsaicin-sensitive, thereby indicating their nociceptive primary afferent.FRAP (fluoride-resistant acid phosphatase), a nonlysozomal enzyme, is localized to small dorsal root ganglion (DRG) neurons. After dorsal root ligation (Knyihar, 1971), dorsal rhizotomy, blocking the axonal flow by microtubule polymerizing agents such as colchicin and vinblastine (Knyihar-Csillik and Csillik, 1981) and capsaicin treatment (Nagy et aL, 1981), FRAP reactivity disappeared in lamina II of the spinal dorsal horn and small DRG neurons. Accordingly, FRAP is believed to be synthesized in small primary afferent neurons and conveyed to the central (Knyihar, 1971) and peripheral (Devor and Claman, 1980) terminals by axonal flow. Thirty to 50% of the DRG cells projecting to the splanchnic or intercostal nerve are FRAPpositive (Dalsgaard et al., 1984). Moreover, numerous unmyelinated axons innervating the corneal epithelium exhibit acid phosphtase activity (Knyihar-Csillik and Csillik, 1981). Therefore, FRAP has been currently used for identification of the primary afferent neurons. The fine structures of FRAP-positive central endings of the primary afferent were extensively documented by KnyiharCsillik and Csillik (1981). The FRAP-positive terminals in the rat substantia gelatinosa of the spinal dorsal horn and caudal spinal trigeminal nucleus are dark sinusoid axons (DSA) or scalloped (fanlike) terminals with packed regular (clear) synaptic vesicles and few or numerous large dense core vesicles. Ribeiro-da-Silva et al. also examined FRAP-positive terminals in the substantia gelatinosa of the rat spinal dorsal horn (1986) and spinal trigeminal nucleus caudalis (1989). They showed that FRAP-positive terminals have electron-dense sinuous or scalloped contours with closely packed regular synaptic vesicles, few large dense core vesicles and mitochondria. Fine structures of the FRAP-positive terminals are the same as the central terminals (CI type), described by Ribeiro-daSilva and Coimbra in 1982, situated centrally forming synaptic glomeruli which are capsaicin...

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Peripheral projections and neuropeptide coexistence in a subpopulation of fluoride-resistant acid phosphatase reactive spinal primary sensory neurons

Cited by 51 publications

References 17 publications

Neuropeptides in sensory perikarya projecting to the rat ovary

Neuropeptides in sensory perikarya projecting to the rat ovary

Noxious Cutaneous Thermal Stimuli Induce a Graded Release of Endogenous Substance P in the Spinal Cord: Imaging Peptide ActionIn Vivo

FRAP-positive and Capsaicin-Sensitive Terminals in the Substantia Gelatinosa of the Mouse Spinal Trigeminal Nucleus Caudalis

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