Neuropeptides in sensory perikarya projecting to the rat ovary

McNeill, Daniel L.; Burden, Hubert W.

doi:10.1002/aja.1001790308

Cited by 29 publications

(7 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1B and 2A), is suggestive of neuronal metaplasia. Although the presence of cholecystokinin-positive nerve fibers has been reported in the interstitium of the normal ovary (32,33), these are thought to originate from neurons situated in the dorsal root ganglia rather than an endogenous ovarian neurosecretory cell population, which is supported by our inability to detect Cck mRNA in normal ovaries (Fig. 1B).…”

Section: Resultssupporting

confidence: 55%

“…Reverse transcription-PCR (RT-PCR) was done using the SuperScript One-Step RT-PCR System with Platinum Taq kit (Invitrogen, Carlsbad, CA), and %100 ng samples of ovarian and GCT total RNA that had been isolated as described above. Reactions were formulated as directed by the manufacturer, except that 0.625 ACi [a- 32 P]dCTP (specific activity, 3,000 Ci/mmol; MP Biomedicals, Irvine, CA) was added to each reaction to generate quantifiable radioactive signal and to increase assay sensitivity. Oligonucleotides used are detailed in Table 1, except those for ribosomal protein L19 (Rpl19), which were as described previously (ref.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Dominant-Stable β-Catenin Expression Causes Cell Fate Alterations and Wnt Signaling Antagonist Expression in a Murine Granulosa Cell Tumor Model

Boerboom¹,

White

Dalle

et al. 2006

Cancer Research

View full text Add to dashboard Cite

Wnt/B-catenin signaling is normally involved in embryonic development and tissue homeostasis, and its misregulation leads to several forms of cancer. We have reported that misregulated Wnt/B-catenin signaling occurs in ovarian granulosa cell tumors (GCT) and have created the Catnb flox(ex3)/+ ; Amhr2 cre/+ mouse model, which expresses a dominant-stable mutant of B-catenin in granulosa cells and develops late-onset GCT. To study the mechanisms leading to GCT development, gene expression analysis was done using microarrays comparing Catnb flox(ex3)/+ ; Amhr2 cre/+ ovaries bearing pretumoral lesions with control ovaries. Overexpressed genes identified in Catnb flox(ex3)/+ ; Amhr2 cre/+ ovaries included the Wnt/B-catenin signaling antagonists Wif1, Nkd1, Dkk4, and Axin2, consistent with the induction of negative feedback loops that counteract uncontrolled Wnt/B-catenin signaling. Expression of the antagonists was localized to cells forming the pretumoral lesions but not to normal granulosa cells. Microarray analyses also revealed the ectopic expression of bone markers, including Ibsp, Cdkn1c, Bmp4, and Tnfrsf11b, as well as neuronal/neurosecretory cell markers, such as Cck, Amph, Pitx1, and Sp5. Increased expression of the gene encoding the cytokine pleiotrophin was also found in Catnb flox(ex3)/+ ; Amhr2 cre/+ ovaries and GCT but was not associated with increased serum pleiotrophin levels. In situ hybridization analyses using GCT from Catnb flox(ex3)/+ ; Amhr2 cre/+ mice revealed that Wnt/B-catenin antagonists and neuronal markers localized to a particular cell population, whereas the bone markers localized to a distinct cell type associated with areas of osseous metaplasia. Together, these results suggest that misregulated Wnt/B-catenin signaling alters the fate of granulosa cells and that the GCT that arise in Catnb flox(ex3)/+ ; Amhr2 cre/+ mice result from the clonal expansion of metaplastic cells. (Cancer Res 2006; 66(4): 1964-73)

show abstract

Section: Resultssupporting

confidence: 55%

Section: Methodsmentioning

confidence: 99%

Dominant-Stable β-Catenin Expression Causes Cell Fate Alterations and Wnt Signaling Antagonist Expression in a Murine Granulosa Cell Tumor Model

Boerboom¹,

White

Dalle

et al. 2006

Cancer Research

View full text Add to dashboard Cite

show abstract

“…We feel this is unlikely, however, since none of the other neuropeptides (CCK-8, substance P, VIP,) we have previously examined with dual labeling approaches in dorsal root ganglia have ever shown similar relationships to cells projecting to the ovary (McNeill and Burden, 1987).…”

Section: Discussionmentioning

confidence: 87%

“…An additional tissue used to control for the specificity of TB-labeling was the right T-13 dorsal root ganglia (DRG). Since retrograde labeling from the rat ovary is ipsilateral in DRG (Inyama et al, 1986;McNeill and Burden, 1987), any labeling in the opposite side at T-13 would indicate a non-specific, bloodborne label and provide a basis for rejection of the animal from the study. Both control tissues were sectioned and processed for visualization of CR-ir cells.…”

Section: Controlsmentioning

confidence: 99%

Localization of calretinin in the rat ovary and in relation to nerve cell bodies in dorsal root and paravertebral ganglia projecting to the ovary

Burden¹,

Zary²

2002

Microscopy Res & Technique

Self Cite

View full text Add to dashboard Cite

Retrograde tracing with True Blue was combined with immunocytochemistry to determine the source of any calretinin-immunoreactive (CR-ir) nerves projecting to the rat ovary. In the ovary, a strong signal for calretinin immunoreactivity was localized in interstitial gland cells; however, no intraovarian CR-ir nerves could be demonstrated. When the superior ovarian nerve was isolated, cut, and True Blue applied to the proximal end, the fluorescent dye was retrogradely transported to a population of cells located in T-12, T-13, and L-1 dorsal root and paravertebral ganglia. There was virtually no dual labeling of cells in these ganglia with calretinin (< 0.009% dual labeling in dorsal root and <0.014% in paravertebral ganglia). However, greater than two-thirds of the True Blue-labeled cells were immediately adjacent to CR-ir cells in dorsal root ganglia. This arrangement is suggestive of a paracrine mechanism between CR-ir cells and cells projecting to the ovary. In paravertebral ganglia, 63% of cells projecting to the ovary were surrounded completely or partially by beaded CR-ir nerve fibers. The source of these fibers (sensory or preganglionic sympathetic) is unknown but hypothesized to be preganglionic. Collectively, these observations suggest a participatory role for calretinin in ovarian function, either directly via effects on the interstitial gland or indirectly by influencing neurons projecting to the ovary.

show abstract

“…Several neurotransmitters and the enzymes participating in their synthesis or degradation (such as noradrenaline (NA), dopamine (DA), serotonin, acetyl-cholinesterase, gamma aminobutiric acid (GABA), VIP, SP, neuropeptide Y (NPY), cholecystokinin-8, the calcitonin gene-related peptide (CGRP), and corticotrophin releasing hormone (CRH)) are present in the fibbers innervating the internal theca of the follicles, the interstitial gland and the ovarian vasculature [14][15][16][17][18][19][20].…”

Section: Endocrinol Metabolmentioning

confidence: 99%

The Ovarian Innervation Participates in the Regulation of Ovarian Functions

Cruz-Morales¹

2012

Endocrinol Metabol Syndrome

View full text Add to dashboard Cite

The release of gonadotropins is the main endocrine signal regulating ovulation and the release of hormones by the ovaries. Several types of growth factors modulate the effects of gonadotropins on the follicular, luteal and interstitial compartments of the ovaries. During the last 30 years, numerous studies have indicated that the ovarian innervations play a role in modulating the effects that gonadotropin have on the ovaries' ability to ovulate and secrete steroid hormones. This literature review presents a summary of the experimental results obtained by analyzing the effects of stimulating or blocking the well-known neural pathways participating in the regulation of ovulation and secretion of steroid hormones. Together, the results suggest that various neurotransmitter systems modulate the effects of gonadotropins on ovulation and the ovaries capacity to secrete steroid hormones. In addition, the ovaries asymmetric capacity for ovulation and hormone secretion could be explained by the asymmetries in their innervations.

show abstract

Neuropeptides in sensory perikarya projecting to the rat ovary

Cited by 29 publications

References 51 publications

Dominant-Stable β-Catenin Expression Causes Cell Fate Alterations and Wnt Signaling Antagonist Expression in a Murine Granulosa Cell Tumor Model

Dominant-Stable β-Catenin Expression Causes Cell Fate Alterations and Wnt Signaling Antagonist Expression in a Murine Granulosa Cell Tumor Model

Localization of calretinin in the rat ovary and in relation to nerve cell bodies in dorsal root and paravertebral ganglia projecting to the ovary

The Ovarian Innervation Participates in the Regulation of Ovarian Functions

Contact Info

Product

Resources

About