Peripheral nerves do not play a trophic role in limb skeletal morphogenesis

Strecker, Teresa R.; Stephens, Trent D.

doi:10.1002/tera.1420270204

Cited by 47 publications

(29 citation statements)

References 11 publications

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“…Data from foil barrier (Stephens and McNulty 1981;Strecker and Stephens 1983) and tissue ablation (Geduspan and Solursh 1992) experiments are consistent with the hypothesis that at stages 13-15, the source of the limb inducer is the IM, which lies between the somite and the lateral plate mesoderm and is comprised of nephrogenic mesoderm (NM) and the Wolffian duct (WD) (Fig. 4C).…”

Section: Source and Identity Of The Endogenous Limb Inducersupporting

confidence: 75%

The roles of FGFs in the early development of vertebrate limbs

Martin¹

1998

Genes Dev.

605

425

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Section: Source and Identity Of The Endogenous Limb Inducersupporting

confidence: 75%

The roles of FGFs in the early development of vertebrate limbs

Martin¹

1998

Genes Dev.

605

425

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“…This suggests a conserved mechanism whereby nerves enter the limb after initial tissue outgrowth from the flank, possibly allowing for myocyte and cartilage differentiation in the aneural environment before rapid innervation proceeds. In agreement with our finding that limb patterning has already begun before nerves enter the limb, in chickens, the presence of nerves is not required for limb patterning (Harsum et al, 2001; Mahony et al, 2018; Strecker and Stephens, 1983). We used two assays to observe neuronal innervation into the mouse fore‐ and hindlimbs: immunofluorescence and immunoperoxidase (Fig.…”

Section: Discussionsupporting

confidence: 91%

Expression analysis of limb element markers during mouse embryonic development

Rafipay

Berg

Erskine

et al. 2018

Developmental Dynamics

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Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exist, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analyzing development of the limb in mutant mice models. Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti‐neurofilament staining of developing nerves in the fore‐ and hindlimbs and cartilage formation and progression also are described. Conclusions: This study demonstrates and describes a range of key morphological markers and methods that together can be used to assess normal and abnormal limb development. Developmental Dynamics 247:1217–1226, 2018. © 2018 The Authors. Developmental Dynamics published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists

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“…Previous studies of limb development have hypothesized that structures medial to the lateral plate mesoderm, such as the intermediate mesoderm, mesonephros, node, somites and segmental plate, produce an axial cue that is required for limb induction (Capdevila and Belmonte, 2001;Crossley et al, 1996;Geduspan and Solursh, 1992;Logan, 2003;Martin, 1998;Saito et al, 2006;Stephens and McNulty, 1981;Strecker and Stephens, 1983). We show that in marsupials both limb fields are specified, as signaled by Tbx expression, before any such medial axial structures exist.…”

Section: Models Of Vertebrate Limb Initiationmentioning

confidence: 58%

Developmental origins of precocial forelimbs in marsupial neonates

2010

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SUMMARYMarsupial mammals are born in an embryonic state, as compared with their eutherian counterparts, yet certain features are accelerated. The most conspicuous of these features are the precocial forelimbs, which the newborns use to climb unaided from the opening of the birth canal to the teat. The developmental mechanisms that produce this acceleration are unknown. Here we show that heterochronic and heterotopic changes early in limb development contribute to forelimb acceleration. Using Tbx5 and Tbx4 as fore-and hindlimb field markers, respectively, we have found that, compared with mouse, both limb fields arise notably early during opossum development. Patterning of the forelimb buds is also accelerated, as Shh expression appears early relative to the outgrowth of the bud itself. In addition, the forelimb fields and forelimb myocyte allocation are increased in size and number, respectively, and migration of the spinal nerves into the forelimb bud has been modified. This shift in the extent of the forelimb field is accompanied by shifts in Hox gene expression along the anterior-posterior axis. Furthermore, we found that both fore-and hindlimb fields arise gradually during gastrulation and extension of the embryonic axis, in contrast to the appearance of the limb fields in their entirety in all other known cases. Our results show a surprising evolutionary flexibility in the early limb development program of amniotes and rule out the induction of the limb fields by mature structures such as the somites or mesonephros.

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Peripheral nerves do not play a trophic role in limb skeletal morphogenesis

Cited by 47 publications

References 11 publications

The roles of FGFs in the early development of vertebrate limbs

The roles of FGFs in the early development of vertebrate limbs

Expression analysis of limb element markers during mouse embryonic development

Developmental origins of precocial forelimbs in marsupial neonates

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