Peripheral nerve regeneration after experimental section in ovine radial and tibial nerves using synthetic nerve grafts, including expanded bone marrow mesenchymal cells: morphological and neurophysiological results

Casanas, J.; Torre, Jaime de la; Soler, Francesc; García, Félix de Miguel; Rodellar, Clementina; Pumarola, M.; Climent, Jana; Soler, Robert; Orozco, Lluís

doi:10.1016/s0020-1383(14)70003-8

Cited by 32 publications

(30 citation statements)

References 23 publications

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“…In the reports using autologous PRP, Ye et al () reported that PRP using as filler of poly(lactic‐co‐glycolic acid) conduit combined with SCs for sciatic nerve in rabbit significantly increased the number of regenerative axon and myelin thickness and improved the CMAP and NCV in electromyography. On the other hand, Casañas et al () reported that PRP using as filler of nerve conduit (NEUROLAC

normalR

, Polyganics, Netherland) for sciatic nerve in sheep did not promote nerve repair.…”

Section: Discussionmentioning

confidence: 99%

Effect of local administration of platelet‐rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model

et al. 2017

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normalR

, Polyganics, Netherland) for sciatic nerve in sheep did not promote nerve repair.…”

Section: Discussionmentioning

confidence: 99%

Effect of local administration of platelet‐rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model

et al. 2017

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“…Mesenchymal stem cells (MSCs) are considered as a promising therapeutic agent in various disorders. The therapeutic value of MSCs was evident in the number of experimental studies [1][2][3][4][5][6] and clinical trials. 7 Although it was initially thought that the therapeutic effects of MSCs depend on their capabilities for cell replacement, many studies have demonstrated that paracrine activity is the predominant mechanism by which MSCs participate in tissue repair.…”

Section: Introductionmentioning

confidence: 99%

Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels

Dąbrowska

Fattore

Karnas

et al. 2018

IJN

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BackgroundMesenchymal stem cells have been shown therapeutic in various neurological disorders. Recent studies support the notion that the predominant mechanism by which MSCs act is through the release of extracellular vesicles (EVs). EVs seem to have similar therapeutic activity as their cellular counterparts and may represent an interesting alternative standalone therapy for various diseases. The aim of the study was to optimize the method of EV imaging to better understand therapeutic effects mediated by EVs.MethodsThe fluorescent lipophilic stain PKH26 and superparamagnetic iron oxide nanoparticles conjugated with rhodamine (Molday ION Rhodamine B™) were used for the labeling of vesicles in human bone marrow MSCs (hBM-MSCs). The entire cycle from intracellular vesicles to EVs followed by their uptake by hBM-MSCs has been studied. The identity of vesicles has been proven by antibodies against: anti-CD9, -CD63, and -CD81 (tetraspanins). NanoSight particle tracking analysis (NTA), high-resolution flow cytometric analysis, transmission electron microscopy (TEM), ELYRA PS.1 super-resolution microscopy, and magnetic resonance imaging (MRI) were used for the characterization of vesicles.ResultsThe PKH26 and Molday ION were exclusively localized in intracellular vesicles positively stained for EV markers: CD9, CD63, and CD81. The isolated EVs represent heterogeneous population of various sizes as confirmed by NTA. The TEM and MRI were capable to show successful labeling of EVs using ION. Co-culture of EVs with hBM-MSCs revealed their uptake by cells in vitro, as visualized by the co-localization of PKH26 or Molday ION with tetraspanins inside hBM-MSCs.ConclusionPKH26 and Molday ION seem to be biocompatible with EVs, and the labeling did not interfere with the capability of EVs to re-enter hBM-MSCs during co-culture in vitro. Magnetic properties of IONs provide an additional advantage for the imaging of EV using TEM and MRI.

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“…Cell-based therapies have been proposed as a promising alternative to treat a variety of neurologic injuries and the use of stem cells that can diferentiate into appropriate cell types in the afected area has developed rapidly in the last years [67]. Stem cells are undiferentiated cells capable to proliferate and produce both new stem cells and diferent types of cells and tissues [68].…”

Section: Neurotrophic Factors Secreted By Neuronal or Non-neuronal Cmentioning

confidence: 99%

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

Alvites¹,

Santos²,

ProençaVarejão³

et al. 2017

Mesenchymal Stem Cells - Isolation, Characterization and Applications

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The peripheral nerve injury after trauma is a common occurrence in both human and veterinary medicine and has severe consequences for the survival and quality of life of the patients. Despite the continuous eforts and the creation of diverse medical and surgical techniques, the harmful efects of this type of injury are far from being overcome. Regenerative medicine has been growing in the scientiic milieu as a new therapeutic approach for diferent situations. Among the cell-based therapies explored, the mesenchymal stem cells are evidenced by their features, versatility and potential applications. The olfactory mucosa mesenchymal stem cells, components of the olfactory system and identiied in the lamina propria, were newly identiied and are still undergoing characterization, appearing as a new promise in the regenerative therapy of several tissues but with special emphasis on the nervous system in general and the peripheral nervous system in particular, for which they appear to have special regenerative aptitude.

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Peripheral nerve regeneration after experimental section in ovine radial and tibial nerves using synthetic nerve grafts, including expanded bone marrow mesenchymal cells: morphological and neurophysiological results

Cited by 32 publications

References 23 publications

Effect of local administration of platelet‐rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model

Effect of local administration of platelet‐rich plasma (PRP) on peripheral nerve regeneration: An experimental study in the rabbit model

Imaging of extracellular vesicles derived from human bone marrow mesenchymal stem cells using fluorescent and magnetic labels

Olfactory Mucosa Mesenchymal Stem Cells and Biomaterials: A New Combination to Regenerative Therapies after Peripheral Nerve Injury

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