Peripheral Lymphocyte DNA Damage and Oxidative Status in Football Players after a Three-day Football Tournament

Atli, Mustafa; Aslan, Mehmet; Küçükoğlu, Mehmet Emin; Temur, H. Bayram; Taşkın, Abdullah; Çelik, Hakim

doi:10.2169/internalmedicine.52.7861

Cited by 4 publications

(3 citation statements)

References 37 publications

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“…The effect of regular exercise on the basal level of DNA damage in volunteers who exercise regularly has also been tested. While some authors report a lower level of DNA damage in lymphocytes of people who perform regular exercise [19,[120][121][122], others did not find any association between exercise and the level of DNA damage [37,123] and some even report a higher level [124]. In this last study, even the H 2 O 2 resistance was lower in sportsmen doing regular exercise, namely rugby players.…”

Section: Physical Activitymentioning

confidence: 52%

Application of the comet assay in human biomonitoring: An hCOMET perspective

Azqueta

Ladeira

Giovannelli

et al. 2020

Mutation Research/Reviews in Mutation Research

114

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The comet assay is a well-accepted biomonitoring tool to examine the effect of dietary, lifestyle, environmental and occupational exposure on levels of DNA damage in human cells. With such a wide range of determinants for DNA damage levels, it becomes challenging to deal with confounding and certain factors are interrelated (e.g. poor nutritional intake may correlate with smoking status). This review describes the effect of intrinsic (i.e. sex, age, tobacco smoking, occupational exposure and obesity) and extrinsic (season, environmental exposures, diet, physical activity and alcohol consumption) factors on the level of DNA damage measured by the standard or enzyme-modified comet assay. Although each factor influences at least one comet assay endpoint, the collective evidence does not indicate single factors have a large impact. Thus, controlling for confounding may be necessary in a biomonitoring study, but none of the factors is strong enough to be regarded a priori as a confounder. Controlling for confounding in the comet assay requires a case-by-case approach. Inter-laboratory variation in levels of DNA damage and to some extent also reproducibility in biomonitoring studies are issues that have haunted the users of the comet assay for years. Procedures to collect specimens, and their storage, are not standardized. Likewise, statistical issues related to both sample-size calculation (before sampling of specimens) and statistical analysis of the results vary between studies. This review gives guidance to statistical analysis of the typically complex exposure, co-variate, and effect relationships in human biomonitoring studies.

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Section: Physical Activitymentioning

confidence: 52%

Application of the comet assay in human biomonitoring: An hCOMET perspective

Azqueta

Ladeira

Giovannelli

et al. 2020

Mutation Research/Reviews in Mutation Research

114

View full text Add to dashboard Cite

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“…The calculation of TOS involved utilizing Equation 2, which relied on the absorbance values from the two readings. For the calculation of the oxidative stress index (OSI), the TOS level was divided by the TAS level as a percentage ratio [30], as outlined in Equation 3. [13].…”

Section: Tas Results = [(δAbs H 2 O) -(δAbs Sample)]/[(δabs H 2 O) -(...mentioning

confidence: 99%

Effects of active video gaming on oxidative stress and antioxidant status in university students

Aygün,

Çakır-Atabek

2023

PES

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Background and Study Aim. Many findings have demonstrated that several life-threatening diseases, including cardiovascular diseases, obesity-related diseases, and certain types of malignancies, can be prevented by exercise. Reactive oxygen species (ROS) may be the direct or associated factor that causes or prevents these diseases. It is well known that a strenuous and high-intensity acute exercise increases ROS production and intensifies oxidative stress. At this point, the importance of physical activity (PA) and exercise in reducing oxidants and improving antioxidant defense system increasing. The purposes of the current study were to investigate the short term (acute) and long term (chronic) effects of active video gaming (AVG) on oxidative stress indices. Material and Methods. Nine healthy male university students participated in the study. The participants played an AVG as vigorous physical activity (MET > 7) for 20 minutes, three days a week, for 4 weeks. Each participant completed twelve game sessions. Blood samples were obtained just before (after 10 min rest in seated position) and immediately after the game session on the first and the last day of the study. Total oxidant and antioxidant status (TOS and TAS) were determined using an automated measurement method, and the oxidative stress index (OSI) was calculated. Total oxidized guanine (TOG) and superoxide dismutase (SOD) activity were analyzed using commercial kits (Cayman Chemical). Data were analyzed with dependent t-tests and significance was accepted as p<0.05. Results. The SOD activity significantly decreased after the AVG on the first day (4.78 ± 1.26 vs. 3.81 ± 1.80; p=0.026). The TOS (6.62 ± 1.09 vs. 7.30 ± 1.24) and TOG (9.26 ± 2.69 vs. 9.78 ± 2.62) levels increased on the first day, but these alterations were not significant. Additionally, no significant acute changes were observed for the last day of the study Conclusions. The 4-week active video gaming practices did not change the oxidative stress status of pre-game, chronically. Four-week-AVG practice did not change oxidative stress indices significantly in rest.

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“…Another finding was that TOS levels in the colostrum were approximately 5 times less than in the blood serum of healthy people (20,21). Both the high persistence of TAS levels in the colostrum relative to blood serum (20) and the up to 5 times less levels of TOS in the colostrum may indicate that antioxidants and oxidants are selectively passed to the colostrum, thereby providing increased evidence of colostrum as important to life support. Older mothers reported significantly higher TOS levels than younger mothers (Table 3).…”

Section: Discussionmentioning

confidence: 99%

Colostrum immunoglobulins and oxidative capacity may be affected by infant sex and maternal age and parity

Kaplan¹,

Bağcı²,

Örkmez³

et al. 2019

Turk J Med Sci

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Background/aim: The aims of this study were to determine the levels of the total antioxidant status (TAS), the total oxidant status (TOS), the oxidative stress index (OSI), and the concentration of immunoglobulin A (IgA) and M (IgM) in colostrum, and evaluate relationships between these parameters and maternal age, maternal parity, and infant sex. Materials and methods: The analysis was performed in serum samples of colostrum which were collected from 90 mothers on the first day of lactation between 10:00 and 12:00 AM. Results: The measurements established that no significant association existed between the TAS level of colostrum and parity, maternal age, or infant sex. However, mothers 18 to 30 years of age had significantly lower colostrum TOS and OSI levels compared with mothers older than 30 years of age. IgA and IgM values of the colostrum of primiparous mothers were significantly higher than those of multiparous mothers, whereas no correlations existed with the age of the mother. Additionally, significantly higher colostrum IgA and IgM values were observed in female infants fed colostrum compared with male infants. Conclusion: In conclusion, sex-based hormonal changes in mothers during pregnancy may be associated with the different colostral immunoglobulin levels for male and female infants.

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Peripheral Lymphocyte DNA Damage and Oxidative Status in Football Players after a Three-day Football Tournament

Abstract: These results indicate that physical activity is associated with increased antioxidant capacity and decreased oxidative stress. Such conditions are important for a healthy life. Further studies are needed to clarify the mechanisms underlying this association.

Cited by 4 publications

References 37 publications

Application of the comet assay in human biomonitoring: An hCOMET perspective

Application of the comet assay in human biomonitoring: An hCOMET perspective

Effects of active video gaming on oxidative stress and antioxidant status in university students

Colostrum immunoglobulins and oxidative capacity may be affected by infant sex and maternal age and parity

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