Peripheral chemerin administration modulates hypothalamic control of feeding

Brunetti, Luigi; Orlando, Giustino; Ferrante, Claudio; Recinella, Lucia; Leone, Sheila; Chiavaroli, Annalisa; Nisio, Chiara Di; Shohreh, Rugia; Manippa, Fabio; Ricciuti, Adriana; Vacca, Michèle

doi:10.1016/j.peptides.2013.11.007

Cited by 53 publications

(34 citation statements)

References 47 publications

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“…The two terpenophenols objects of this study were also ineffective against the DA level, whereas the sole CBD stimulated 5-HT levels, and this could explain, albeit partially, the aforementioned anorexigenic effects [14]. The evaluation of the biogenic amine steady state level is currently considered a useful tool to predict the effect of a drug on the activity in the brain, particularly in in vivo studies [44,45]. Nevertheless, other experimental paradigms, such as microdialysis/push-pull, synaptosome perfusion, and cell cultures can give a more detailed assessment with regard to the effects of drugs on brain neurotransmitter release.…”

Section: Discussionmentioning

confidence: 84%

Neuroprotective and Neuromodulatory Effects Induced by Cannabidiol and Cannabigerol in Rat Hypo-E22 cells and Isolated Hypothalamus

et al. 2020

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Background: Cannabidiol (CBD) and cannabigerol (CBG) are non-psychotropic terpenophenols isolated from Cannabis sativa, which, besides their anti-inflammatory/antioxidant effects, are able to inhibit, the first, and to stimulate, the second, the appetite although there are no studies elucidating their role in the hypothalamic appetite-regulating network. Consequently, the aim of the present research is to investigate the role of CBD and CBG in regulating hypothalamic neuromodulators. Comparative evaluations between oxidative stress and food intake-modulating mediators were also performed. Methods: Rat hypothalamic Hypo-E22 cells and isolated tissues were exposed to either CBD or CBG, and the gene expressions of neuropeptide (NP)Y, pro-opiomelanocortin (POMC) and fatty acid amide hydrolase were assessed. In parallel, the influence of CBD on the synthesis and release of dopamine (DA), norepinephrine (NE), and serotonin (5-HT) was evaluated. The 3-hydroxykinurenine/kinurenic acid (3-HK/KA) ratio was also determined. Results: Both CBD and CBG inhibited NPY and POMC gene expression and decreased the 3-HK/KA ratio in the hypothalamus. The same compounds also reduced hypothalamic NE synthesis and DA release, whereas the sole CBD inhibited 5-HT synthesis. Conclusion: The CBD modulates hypothalamic neuromodulators consistently with its anorexigenic role, whereas the CBG effect on the same mediators suggests alternative mechanisms, possibly involving peripheral pathways.Funding: This study was entrusted by Enecta B.V. (Corantijnstraat 5-1, 1058DA Amsterdam, The Netherlands) within the project entitled "Effects of cannabidiol and cannabigerol on the neuroendocrine mechanisms underlying feeding behavior and energy balance" (Coordinators: Claudio Ferrante, Giustino Orlando and Luigi Menghini).

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Section: Discussionmentioning

confidence: 84%

Neuroprotective and Neuromodulatory Effects Induced by Cannabidiol and Cannabigerol in Rat Hypo-E22 cells and Isolated Hypothalamus

et al. 2020

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“…). Multiple comparative studies confirmed that neurotransmitter steady state level is a valuable index of neurotransmitter release in vivo (Brunetti et al ., ). Actually, our findings of reduced colon 5‐HT levels induced by chamomile extract is consistent with the concentration‐dependent apigenin‐induced inhibition of gut 5‐HT release, in vitro (Zhao et al ., ).…”

Section: Discussionmentioning

confidence: 97%

An Hydroalcoholic Chamomile Extract Modulates Inflammatory and Immune Response in HT29 Cells and Isolated Rat Colon

et al. 2016

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Inflammatory bowel diseases (IBDs) are chronic disorders characterized by disruption and ulceration of the colonic mucosa or of any part of the digestive tract (Crohn's disease). Antioxidant/anti-inflammatory herbal extract supplementation could represent an innovative approach to contrast IBDs. Clinical trials demonstrated the efficacy of natural formulas, containing chamomile, in patients with gastrointestinal disorders. This is consistent, albeit in part, with the antioxidant and anti-inflammatory properties of chamomile. The aim of the present study was to explore the possible protective role of a chamomile extract, on human colorectal adenocarcinoma HT29 cell, and rat colon specimens treated with lipopolysaccharide (LPS) to induce an inflammatory stimulus, a well established model of acute ulcerative colitis. In this context, the activities of different biomarkers of inflammation and lipid peroxidation such as ROS, myeloperoxidase (MPO), serotonin (5-HT), prostaglandin (PG)E2 , 8-iso-prostaglandin (8-iso-PG)F2α , NF-kB, tumor necrosis factor (TNF)α and interleukin (IL)-6 were assessed. We found that chamomile extract was as effective as sulfasalazine (2 mg/ml) in reducing the production of MPO, 5-HT, IL-6, NF-kB, TNFα, PGE2 and 8-iso-PGF2α , after inflammatory stimulus. The observed modulatory effects support a rationale use of chamomile supplementation as a promising pharmacological tool for the prevention and management of ulcerative colitis in humans. Copyright © 2016 John Wiley & Sons, Ltd.

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“…On the contrary, isolated colons were explanted and tissue 5-HT (ng/mg wet tissue) was extracted with perchloric acid 0.05 M (Brunetti et al, 2014). Thereafter, neurotransmitter level was determined by HPLC coupled to coulometric detection (Brunetti et al, 2014;Ferrante et al, 2016).…”

Section: Ex Vivo Studiesmentioning

confidence: 99%

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

et al. 2019

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The phytochemical, antiradical, and enzyme inhibition profile of three solvent extracts (ethyl acetate, methanol, water) of Origanum sipyleum were assessed. We also performed a pharmacological study in order to explore protective effects induced by extracts in inflamed colon. LC‐MS analysis revealed that the extracts contained different classes of phenolics. The aqueous extract showed the highest antioxidant and acetylcholinesterase (AChE) inhibitory effects. Total phenol and flavonoid contents were highest in aqueous and ethyl acetate extract, respectively. All extracts were effective in reducing colon pro‐oxidant and pro‐inflammatory biomarkers. The extracts revealed also able to inhibit fungal and bacterial species involved in ulcerative colitis, including Candida albicans, Candida tropicalis, Staphylococcus aureus, and Staphylococcus thyphimurium. Finally, we also showed the antiproliferative effects exerted by the EA extracts on human colon cancer HCT116 cell line. Concluding, our results indicated that O. sipyleum extracts displayed promising therapeutic properties which warrants further validation. Practical applications The present phytochemical and biological studies, including antioxidant, anti‐inflammatory, and antimicrobic assessments, showed significant protective effects exerted by O. sipyleum extracts in an experimental model of ulcerative colitis. The results are intriguing and suggest potential applications O. sipyleum extracts as sources of natural agents for the management of clinical symptoms related to ulcerative colitis, characterized by increased burden of oxidative stress and microbiome dysbiosis.

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Peripheral chemerin administration modulates hypothalamic control of feeding

Cited by 53 publications

References 47 publications

Neuroprotective and Neuromodulatory Effects Induced by Cannabidiol and Cannabigerol in Rat Hypo-E22 cells and Isolated Hypothalamus

Neuroprotective and Neuromodulatory Effects Induced by Cannabidiol and Cannabigerol in Rat Hypo-E22 cells and Isolated Hypothalamus

An Hydroalcoholic Chamomile Extract Modulates Inflammatory and Immune Response in HT29 Cells and Isolated Rat Colon

Chemical profiling and pharmaco‐toxicological activity ofOriganum sipyleumextracts: Exploring for novel sources for potential therapeutic agents

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