Peripheral blood cells of native pig (Zovawk) of Mizoram, India: Light and scanning electron microscopy analysis

Abstract

The literature on the blood cells of Zovawk pigs is scanty; thus, this study was designed to elaborate the light microscopy (LM) and scanning electron microscopy (SEM) characterization of blood cells of Zovawk pigs by LM and SEM. Twelve different blood samples were aseptically obtained from adult healthy Zovawk pigs, irrespective of sex. To determine the cytomorphological and cytochemical structures of the many produced constituents of blood, blood smears were stained with various stains. The blood samples were treated with various substrates for cytoenzymatic research, and the alterations were noted. A 1000× magnification Olympus Trinocular Research microscope was used to examine the smears. The blood samples were prepared for electron microscopy according to the standard procedure. The prepared samples were delivered to the Sophisticated Analytical Instrumentation Facility (SAIF), All India Institute of Medical Sciences (AIIMS), New Delhi, for SEM imaging. On LM, pig erythrocytes were spherical and nonnucleated. The cytoplasm of the neutrophils was spherical and included cytoplasmic granules. The eosinophils had prominent cytoplasmic granules and were round. Basophils were infrequently present and had cytoplasmic granules that were clear blue. The sizes of small, medium and large lymphocytes were noted. The monocytes were oval or circular. The platelets ranged in form from asymmetric to round. The blood samples were stained for cytochemical analyses using acid ferrocyanide stain for iron, Sudan black blue stain for lipids, toluidine blue stain for mucopolysaccharides, and periodic acid Schiff's stain for glycogen. The cytoenzymatic characteristics were evaluated and compared with substrates treated with acid phosphatase, alkaline phosphatase, peroxidase, arylsulfatase, cytochrome oxidase, beta‐glucuronidase, and succinate dehydrogenase. Erythrocytes appeared as biconcave disks under SEM. Two forms of leukocytes were observed, having a rough and pointed cell surface like a flower. It can be concluded that the LM and SEM morphology of blood cells of Zovawk pigs resembled other domestic animals, however, few differences were observed among the discussed animals.

Light microscopic morphology of blood cells of non‐descript indigenous Zoar chicken of Mizoram, India

“…Routine examination of blood to assess the general health of an individual has become the gold standard for clinical veterinary practices (Choudhary et al., 2021; Choudhary, Kalita, et al., 2023; Choudhary, Sarkar, et al., 2023; Sarkar et al., 2022) in the era of technological advancements in veterinary anatomy (Choudhary, Saini, & Challana, 2023). As a result, many studies have been carried out on the blood biochemical profiles to evaluate their immune responses to varying levels of stress.…”

Diagnostic Importance of Cytochemical and Cytoenzymatic Patterns of Peripheral Blood Cells in Native Cattle (Zobawng) of Mizoram, India

“…Blood is a fluid connective tissue in which plasma is suspended together with produced elements such as erythrocytes, granulocytes (neutrophils, eosinophils and basophils), agranulocytes (monocytes and lymphocytes) and platelets (Atkins et al, 2017;Choudhary et al, 2021). Blood is tested regularly to detect hematological problems and diagnose illnesses (Choudhary et al, 2022). In some disorders, blood cell identification and awareness of species differences in terms of morphological distinction are also required.…”

Morphologic appearance of peripheral blood cells from Zovawk pigs (Sus scrofa domesticus) visualized by transmission electron microscopy

“…7,8 Zovawk pig platelets (Figures 3C,D) are non-nucleated, roughly round to elongated, with few pseudopods at the cell surface. 13 The ultrastructure of the platelets is divided into three zones: (a) a peripheral zone with a glycocalyx surface coating, a membrane, and a membrane cytoskeleton, (b) a sol-gel zone, and (c) a granular zone.…”