Diagnostic Importance of Cytochemical and Cytoenzymatic Patterns of Peripheral Blood Cells in Native Cattle (Zobawng) of Mizoram, India

Abstract

Background: The cytochemistry and cytoenzymatic profile of the blood cells is an important tool in the hands of clinical veterinary practitioners. It has also applications in various fields of veterinary sciences. Therefore based on its clinical importance present study was conducted on the blood cells of native cattle (Zobawng) of Mizoram to examine the cytochemical and cytoenzymatic characteristics. Methods: For this study, a total of 12 numbers of blood samples (5 ml) were taken aseptically from adult local cattle of Mizoram, irrespective of sex. The blood smears were prepared and stained with acid ferrocyanide (ACF) for the detection of non-heame iron, toluidine blue (TBO) stain for acid mucopolysaccharides, Periodic Acid-Schiff (PAS) stain for observation of glycogen and Sudan black B (SBB) stain for demonstration of lipid. Acid phosphatase (ACP), alkaline phosphatase (ALP), peroxidase (POX), arylsulphatase (ARS), cytochrome oxidase (CYO), beta-glucuronidase (GUSB) and succinate dehydrogenase (SDH) enzymes were used for cytoenzymatic features of blood cells. The smears were observed under 1000X magnification in an Olympus Trinocular Research microscope. Result: In the cytochemical studies, basophils showed a positive reaction for mucopolysaccharides in the toluidine blue stain and neutrophils, eosinophils and basophils recorded positive for glycogen in the PAS stain. Eosinophils and basophils also displayed positive for lipids in the SBB stain. The cytoenzymatic studies of eosinophils and basophils recorded positive reactions for ACP, ALP, POX, ARS and CYO activity.