Periostin contributes to epidermal hyperplasia in psoriasis common to atopic dermatitis

Abstract

Background Epidermal hyperplasia is a histological hallmark observed in both atopic dermatitis (AD) and psoriasis, although the clinical features and the underlying immunological disorders of these diseases are different. We previously showed that periostin, a matricellular protein, plays a critical role in epidermal hyperplasia in AD, using a mouse model and a 3-dimensional organotypic coculture system. In this study, we explore the hypothesis that periostin is involved in epidermal hyperplasia in psoriasis. Methods To examine expression of periostin in psoriasis patients, we performed immunohistochemical analysis on skin biopsies from six such patients. To investigate periostin’s role in the pathogenesis of psoriasis, we evaluated periostin-deficient mice in a psoriasis mouse model induced by topical treatment with imiquimod (IMQ). Results Periostin was substantially expressed in the dermis of all investigated psoriasis patients. Epidermal hyperplasia induced by IMQ treatment was impaired in periostin-deficient mice, along with decreased skin swelling. However, upon treatment with IMQ, periostin deficiency did not alter infiltration of inflammatory cells such as neutrophils; production of IL-17, –22, or –23; or induction/expansion of IL-17– and IL-22–producing group 3 innate lymphoid cells. Conclusions Periostin plays an important role during epidermal hyperplasia in IMQ-induced skin inflammation, independently of the IL-23–IL-17/IL-22 axis. Periostin appears to be a mediator for epidermal hyperplasia that is common to AD and psoriasis.

Protease-Activated Receptor-2: A Multifaceted Molecular Transducer in the Human Skin

“…Additionally, Shin et al1 found that human epidermal keratinocytes (SV-HEKs), which were fully differentiated displayed significantly higher PAR-2 expression than undifferentiated keratinocytes, which is in accordance with similar previous findings. This corresponds well with the fact that both aforementioned skin conditions; atopic dermatitis and psoriasis involves substantial epidermal hyperplasia5, in addition to PAR-2 associated sensory symptoms, most prominently itch, cutaneous inflammation and mild pain.…”

Tannic acid modulates NFκB signaling pathway and skin inflammation in NC/Nga mice through PPARγ expression

“…The remaining excised skin was cut into about 2 mm thick transverse slices and fixed in 10% formalin. Sections of 3-5 lm thickness were stained with haematoxylin and eosin (H&E), naphthol AS-D chloroacetate esterase and toluidine blue for detecting epidermal thickness [15], inflammatory cells such as neutrophil and mast cells, respectively. A histomorphological evaluation of all the skin sections was carried out in a blinded fashion [14].…”

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“…Quantitative RT‐PCR was performed as previously described . Briefly, total RNA was prepared using an RNAiso plus reagent (Takara Bio, Otsu, Japan), and reverse‐transcribed with the ReverTra Ace (TOYOBO, Osaka, Japan).…”