Periosteum-Inspired Membranes Integrated with Bioactive Magnesium Oxychloride Ceramic Nanoneedles for Guided Bone Regeneration

Abstract

Guided bone regeneration (GBR) technique using a barrier membrane holds great potential to allow the single-stage reconstruction of critical-sized bone defects. Here, bioactive nanoneedle-like magnesium oxychloride ceramics (MOCs) are synthesized and recruited as an osteoinductive factor within a polycaprolactone-gelatin A (PCL-GelA) membranous matrix to generate a periosteum-mimicking biphasic GBR membrane (PCL-GelA/MOC) to accelerate calvarial defect repair. The PCL-GelA/MOC membrane acts as a shield for defect areas and a reservoir of osteoinductive molecules, which provides a favorable microenvironment for supporting cell proliferation, infiltration, and differentiation. This membrane leads to accelerated osteogenesis and angiogenesis, effectual defect bridging, and significantly enhanced bone regeneration when applied to a 5 mm sized rat calvarial defect. This makes this innovative and multifunctional GBR membrane a suitable candidate for clinical applications with promising curative efficacy.

Accumulative Rolling Mg/PLLA Composite Membrane with Lamellar Heterostructure for Enhanced Bacteria Inhibition and Rapid Bone Regeneration

“…Furthermore, we assessed the expression of angiogenic marker (vascular endothelial growth factor (VEGF)) and tube formation ability of the membrane (Figure 5G), as new blood vessels work as a bridge linking fresh tissue and original tissue. [ 42 ] The AR5‐ group showed significantly higher expression of OCN and Osterix than the Ctrl‐ and AR0‐ groups (Figure 5H, p < 0.05). With the help of PTT, the levels of almost all involved osteogenic and angiogenic markers are up‐regulated in the AR5+ group compared to the AR5‐ group ( p < 0.05) (Figure 5H–J).…”

Multiphasic Membranes/Scaffolds for Periodontal Guided Tissue Regeneration

“…[30] Considering that alveolar bone loss can often occur in the context of periodontitis or tooth-replacement procedure, many studies have devoted to regenerating the defective bony tissues (Table 1). [15,[31][32][33][34][35] To endow the membrane with additional osteo-promotive properties, therapeutic additives would further be incorporated, like bioceramics (e.g., magnesium-containing bioceramics, hydroxyapatite and calcium phosphate) or growth factors (e.g., bone morphogenic proteins). Recently, our group has reported an asymmetric periosteum-mimicking guided bone regeneration (GBR) membrane through a two-step electrospinning procedure, whereby the blend of polycaprolactone (PCL) and gelatin A (GelA) was the membrane matrix and the magnesium oxychloride ceramic (MOC) nanoneedle was the deliverable osteoinductive factor.…”

Metal ions: the unfading stars of bone regeneration—from bone metabolism regulation to biomaterial applications

“…Bioceramics have been proved to be capable of steadily releasing various metal ions, including Sr 2+ , Cu 2+ , Ca 2+ , and Mg 2+ . 83,264–266 Notably, the controversial Fe 3+ has demonstrated advantageous effects when incorporated into bioceramics. Ullah et al 267 used modified bioceramics with Sr 2+ and Fe 3+ , demonstrating excellent biocompatibility and a favorable microenvironment for bone regeneration.…”