Periodontal wound healing/regeneration following the application of rhGDF‐5 in a <i>β</i>‐TCP/PLGA carrier in critical‐size supra‐alveolar periodontal defects in dogs

Kwon, David H.; Bisch, Frederick C.; Herold, Robert W.; Pompe, Cornelius; Bastone, Patrizia; Rodriguez, Nancy A.; Susin, Cristiano; Wikesjö, Ulf M.E.

doi:10.1111/j.1600-051x.2010.01569.x

Cited by 31 publications

(29 citation statements)

References 52 publications

(45 reference statements)

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“…The early phase lasts for 48-72 h and is characterized by tissue infiltration with PMNs, which are recruited as a first-line defense against infection [18]. At the time of first biopsy retrieval, the early response has already progressed into the late inflammatory response with the macrophage being the key cell.…”

Section: Discussionmentioning

confidence: 99%

Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

2011

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This study's aim was to clarify the influence of soft tissue management on the development of periimplant infection. Four weeks after removal of all maxillary premolars in 12 mini-pigs, four BEGO Semados RI implants were inserted in each maxillary quadrant. Employing a split-mouth design, one quadrant was randomized to flapless insertion while the contralateral side was chosen for flap surgery. Following 1, 2, 4 and 12 weeks of transmucosal implant, healing biopsies were retrieved from the periimplant soft tissue and subjected to further analysis. Histomorphometrically, a significant reduction of transmigration of polymorphonuclear neutrophils (week 1, p = 0.007; week 2, p = 0.021; week 4, p = 0.023; week 12, p = 0.013) as well as the density of the subepithelial inflammatory infiltrates (week 1, p = 0.007; week 2, p = 0.046; week 4, p = 0.003; week 12, p = 0.032) was verified following flapless surgery. Quantification of inducible nitric oxide synthase showed significantly reduced expression in the flapless group 2 (p = 0.027), 4 (p = 0.005) and 12 (p = 0.004) weeks post-insertion. Analysis of CD31 and collagen I immunostained sections revealed more regular capillary distribution as well as higher vessel and collagen density in the flapless group. The data of the present study indicate that flapless placement reduces the incidence of inflammatory periimplant soft tissue lesions during a 12-week period. Considering the beneficial effects of flapless placement on early soft tissue healing and stability, the technique might be preferred in case of an uncomplicated locoregional anatomy with sufficient hard and soft tissue. However, this positive effect might disappear after manipulation of the implant and soft tissue during impression taking or try in of the prosthodontic supraconstruction.

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Section: Discussionmentioning

confidence: 99%

Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

2011

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“…Bilateral supra‐alveolar critical‐sized periodontal defects, 3.0 mm width × 6.0 mm depth, in the mandibular premolar region result in critical‐sized defects in an 8 week time window (Kwon et al ., ), but heal completely after 9 months (Cuisinier et al ., ) (Figure A).…”

Section: Preclinical Evaluation Of Bone Defect Healing and Bone Augmementioning

confidence: 99%

Preclinical evaluation of injectable bone substitute materials

Bongio

Beucken

Leeuwenburgh

et al. 2012

J Tissue Eng Regen Med

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Injectable bone substitutes (IBSs) represent an attractive class of ready-to-use biomaterials, both ceramic- and polymer-based, as they offer the potential benefit of minimally invasive surgery and optimal defect filling. Although in vitro assessments are the first step in the process of development, the safety and efficacy of an IBS strongly depend on validated preclinical research prior to clinical trials. However, the selection of a suitable preclinical model for performance evaluation of an IBS remains a challenge, as no gold standard exists to define the best animal model. In order to succeed in this attempt, we identified three stages of development, including (a) proof-of-principle, (b) predictive validity and (c) general scientific legitimacy, and the respective criteria that should be applied for such selection. The second part of this review provides an overview of commonly used animals for IBSs. Specifically, scientific papers published between January 1996 and March 2012 were retrieved that report the use of preclinical models for the evaluation of IBSs in situations requiring bone healing and bone augmentation. This review is meant not only to describe the currently available preclinical models for IBS application, but also to address critical considerations of such multi-factorial evaluation models (including animal species, strain, age, anatomical site, defect size and type of bone), which can be indicative but in most cases edge away from the human reality. Consequently, the ultimate goal is to guide researchers toward a more careful and meaningful interpretation of the results of experiments using animal models and their clinical applications.

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“…Previous studies have shown that biodegradable synthetic polymers combined with BMPs induce bone formation in animal models; poly‐lactic‐co‐glycolic‐acid (PLGA) microsphere or capsule assemblies have been considered for BMP‐2; however, insufficient mechanical properties limits their use to inlay indications (Kenley et al 1994, Sigurdsson et al 1995, 1996, Isobe et al 1996). More recently, injectable, ease‐of‐use micro/macro‐porous PLGA composites featuring structural integrity upon setting have been considered and combined with rhGDF‐5 (Herberg et al 2008, Kwon et al 2010a,b). The purpose of this study was to evaluate the effect of rhGDF‐5 on periodontal wound healing/regeneration using an injectable PLGA composite carrier and an established defect model.…”

mentioning

confidence: 99%

Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs

Min

Wikesjö

Park

et al. 2011

Journal of Clinical Periodontology

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Periodontal wound healing/regeneration following the application of rhGDF‐5 in a β‐TCP/PLGA carrier in critical‐size supra‐alveolar periodontal defects in dogs

Abstract: Within the limitations of this study, the results suggest that rhGDF-5 is a promising candidate technology in support of periodontal wound healing/regeneration. Carrier and rhGDF-5 dose optimization are necessary before further advancement of the technology towards clinical evaluation.

Cited by 31 publications

References 52 publications

Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

Analysis of inflammatory periimplant lesions during a 12-week period of undisturbed plaque accumulation—a comparison between flapless and flap surgery in the mini-pig

Preclinical evaluation of injectable bone substitute materials

Wound healing/regeneration using recombinant human growth/differentiation factor-5 in an injectable poly-lactide-co-glycolide-acid composite carrier and a one-wall intra-bony defect model in dogs

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