Periodontal Wound Healing by Transplantation of Jaw Bone Marrow‐Derived Mesenchymal Stem Cells in Chitosan/Anorganic Bovine Bone Carrier Into One‐Wall Infrabony Defects in Beagles

Zang, Shengqi; Jin, Lei; Kang, Shuai; Hu, Xin; Wang, Meng; Wang, Jinjin; Chen, Bin; Peng, Bo; Wang, Qintao

doi:10.1902/jop.2016.150504

Cited by 27 publications

(21 citation statements)

References 45 publications

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“…The referred experiment used acute periodontal defects, which have a significantly greater capacity to self‐regenerate as compared to chronic supracrestal defects utilized in this study. In another experimental study, using bone marrow mesenchymal stem cells (BMMSCs) seeded on chitosan/anorganic bovine bone for treating acute surgically created one‐wall critical size infrabony periodontal reported a regeneration of 38% of total defect (Zang et al., ). The amount of periodontal regeneration achieved within the furcation (2.35 mm and 1.93 mm in control and test groups, respectively) can also be comparable with other experimental studies evaluating guided tissue regeneration with barrier membranes of expanded polytetrafluoethylene (e‐PTFE) (Lindhe et al., ; Polimeri et al., ) or with a combination of enamel matrix proteins and a bioabsorbable membrane (Donos, Sculean, Glavind, Reich, & Karring, ).…”

Section: Discussionmentioning

confidence: 99%

Cell therapy with allogenic canine periodontal ligament‐derived cells in periodontal regeneration of critical size defects

Núñez

Sánchez

Vignoletti

et al. 2018

J Clinic Periodontology

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Section: Discussionmentioning

confidence: 99%

Cell therapy with allogenic canine periodontal ligament‐derived cells in periodontal regeneration of critical size defects

Núñez

Sánchez

Vignoletti

et al. 2018

J Clinic Periodontology

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“…Masson's trichrome staining performed by Sukpaita et al [33] found an increased amount of collagen and bone matrix in CS/Dicarboxylic acid scaffold with and without PDLCs seeding. In the study by Zang et al [38], the same test showed more dense and well-organized PDLCs in the chitosan scaffold with hJBMMSCSs than the chitosan/anorganic bovine bone and pure chitosan groups. ARS staining of hPDLCs performed by Xue et al [36] recorded more mineralized nodules on the nPLGA/nCS/nAG complex than in negative control group, showing the that this type of membrane may promote cell mineralization.…”

Section: Results Of Individual Studiesmentioning

confidence: 81%

“…ALP activity of periodontal ligament cells [21], osteoblasts [34,35], and mesenchymal stem or stromal cells [24] was assessed in four studies (Table 5). Three items analyzed the mineralization level of the newly formed bone with the Masson's trichrome staining method [27,33,38], two assessed it with the ARS staining [31,36] and one with the immunofluorescent staining technique for osteocalcin (OCN) [39] ( Table 6). Twelve of the selected papers performed their experiment in vivo, creating bone defects, which were later covered by CS-based scaffolds: Ge et al [21] created bilateral parietal bone defects (with a diameter of 5 mm) in eighteen eight-week-old rats (weight = 180-220 g), scoring the anesthetized cranial skin, exposing calvaria; parietal cranial 15 mm oval-shaped defects were obtained by Jayash et al [25] using a bone trephine drill.…”

Section: Results Of Individual Studiesmentioning

confidence: 99%

“…Shah et al [32] tested the CS-based scaffold on a subcutaneous pouch of eight-week healthy adult rats weighing 140-180 g. Xue et al [36] anesthetize intramuscularly 3 white rabbits (4-6 months, weight 2-3 kg), exposing the lower edge of the mandible and creating a bone defect in the molar area of the mandibular body. Zang et al (2016) [38], used one-wall, box-shaped, infrabony defects (4 mm width, 7 mm depth) at the distal and mesial aspects of the third premolars and at the mesial aspects of the first molars. In the study of 2019 by the same author, bilateral class III furcation defects (4 mm wide and 5 mm high) were created on the third and fourth mandibular premolars [39].…”

Section: Results Of Individual Studiesmentioning

confidence: 99%

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Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

et al. 2020

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Introduction. Several biomaterials are used in periodontal tissue engineering in order to obtain a three-dimensional scaffold, which could enhance the oral bone regeneration. These novel biomaterials, when placed in the affected area, activate a cascade of events, inducing regenerative cellular responses, and replacing the missing tissue. Natural and synthetic polymers can be used alone or in combination with other biomaterials, growth factors, and stem cells. Natural-based polymer chitosan is widely used in periodontal tissue engineering. It presents biodegradability, biocompatibility, and biological renewability properties. It is bacteriostatic and nontoxic and has hemostatic and mucoadhesive capacity. The aim of this systematic review is to obtain an updated overview of the utilization and effectiveness of chitosan-based scaffold (CS-bs) in the alveolar bone regeneration process. Materials and Methods. During database searching (using PubMed, Cochrane Library, and CINAHL), 72 items were found. The title, abstract, and full text of each study were carefully analyzed and only 22 articles were selected. Thirteen articles were excluded based on their title, five after reading the abstract, twenty-six after reading the full text, and six were not considered because of their publication date (prior to 2010). Quality assessment and data extraction were performed in the twelve included randomized controlled trials. Data concerning cell proliferation and viability (CPV), mineralization level (M), and alkaline phosphatase activity (ALPA) were recorded from each article Results. All the included trials tested CS-bs that were combined with other biomaterials (such as hydroxyapatite, alginate, polylactic-co-glycolic acid, polycaprolactone), growth factors (basic fibroblast growth factor, bone morphogenetic protein) and/or stem cells (periodontal ligament stem cells, human jaw bone marrow-derived mesenchymal stem cells). Values about the proliferation of cementoblasts (CB) and periodontal ligament cells (PDLCs), the activity of alkaline phosphatase, and the mineralization level determined by pure chitosan scaffolds resulted in lower than those caused by chitosan-based scaffolds combined with other molecules and biomaterials. Conclusions. A higher periodontal regenerative potential was recorded in the case of CS-based scaffolds combined with other polymeric biomaterials and bioceramics (bio compared to those provided by CS alone. Furthermore, literature demonstrated that the addition of growth factors and stem cells to CS-based scaffolds might improve the biological properties of chitosan.

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“…BMSC were used in 3 studies [58] [59], one of which was a one walled intra bony defect [60], PDLSC in 2 studies [61] [62], DPSC in 1 study [49] and adipose derived stem cell in one study [63] successfully. PDLSC were also used to treat periodontal fenestration defects and was able to form bone, PDL and cementum successfully [64].…”

Section: Furcation Defectsmentioning

confidence: 99%

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

Kanwal¹,

D’souza²,

Muthiah³

et al. 2017

OJST

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Aim: 1) To review the literature of various applications of stem cell regeneration in dentistry from 2010 to 2016. 2) To review these studies and to summarize the current status of stem cell regeneration in intra oral applications.3) To present the available successful data with regard to stem cell regeneration in dentistry and to highlight the future trends. Materials and Methods: Search Protocol: A systematic search was made in the PubMed database with the key words gingiva, papillary reconstruction, periodontal ligament, dental pulp, salivary gland, enamel re-mineralization, dentin, cementum, bone, whole tooth, cleft palate, regeneration and stem cells. All articles from 2010 to 2016, relevant to the topic were included. After the search a total of 1826 articles were obtained which were screened and categorized by three independent reviewers as review papers, in vitro, animal and human studies, pertaining to stem cell regeneration in intra oral applications. On the basis of the extracted data and outcome measures, conclusions were drawn. Results: Maximum number of animal studies has been done to regenerate periodontal tissues, bone, dentin and pulp tissues. Few human studies have been done till date. Although clinically, researchers have been able to regenerate periodontal-like tissue, bone and pulp-like tissue, they still haven't been able to regenerate tissues that completely resemble tissues in their natural form. Conclusion: The future of stem cell therapy in dental applications looks promising. The predictability and efficacy of outcomes, as well as safety concerns of stem cell therapy is yet to be fully established. Ongoing research and development of newer scaffolds, understanding various signaling molecules and their cues, understanding gene expression and proteomics of stem cells are the future directions that will take us a step forward to achieving successful regeneration.

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Periodontal Wound Healing by Transplantation of Jaw Bone Marrow‐Derived Mesenchymal Stem Cells in Chitosan/Anorganic Bovine Bone Carrier Into One‐Wall Infrabony Defects in Beagles

Abstract: The combination of hJBMMSCs and C/ABB scaffolds could promote periodontal repair. Future studies are expected to further optimize the combination and lead to an ideal periodontal regeneration.

Cited by 27 publications

References 45 publications

Cell therapy with allogenic canine periodontal ligament‐derived cells in periodontal regeneration of critical size defects

Cell therapy with allogenic canine periodontal ligament‐derived cells in periodontal regeneration of critical size defects

Nanomaterials for Periodontal Tissue Engineering: Chitosan-Based Scaffolds. A Systematic Review

The Current Status of Stem Cell Regeneration in Intra Oral Applications—A Systematic Review

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