Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty

Chen, Y. L.; Chen, P. K.T.; Jeng, Long-Bin; Huang, Chi-Hsien; Yang, Lin-Cheng; Chung, Hui Ying; Chang, Sophia Chia-Ning

doi:10.1038/gt.2008.131

Cited by 60 publications

(36 citation statements)

References 44 publications

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“…38,39 Moreover, it has been reported that the virally transduced MSCs expressing BMP4 or BMP2 can promote more complete bone healing or periodontal regeneration in vivo. 40,41 Our results showed that a cementum/PDL-like complex was observed only in BT-PF transplants in which collagen fibers had connected the newly formed cementumlike tissues that mimicked physiological attachments of Sharpey's fibers. To distinguish whether the exogenous cells or host cells were responsible for the formation of the cementum/PDL-like structures, we investigated the expressions of human Col I (hCol I), hPOSTN and hOPN immunohistochemically on serial sections.…”

Section: Differentiation Of Periodontal Ligament Fibroblasts H-w MI Ementioning

confidence: 69%

Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer

et al. 2011

Gene Ther

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Because periodontal ligament (PDL) cells are reported to contain progenitor or stem cell populations, they are considered a beneficial cell source for clinical periodontal regeneration. Both bone morphogenetic protein 4 (BMP4) and human telomerase reverse transcriptase (hTERT) have essential roles in the modulation of stem cell properties. In this study we report for the first time that the combined ectopic expression of BMP4 and hTERT significantly enhanced the multipotent differentiation efficiency and capacity of human PDL fibroblasts (PFs), as shown by osteogenic, adipogenic and neurogenic differentiation in vitro, and cementum/PDL-like tissue regeneration in vivo. These findings may be attributed, at least in part, to the original upregulation of important stem cell markers, such as scleraxis, Stro-1 and CD146, and the extremely lowered threshold for BMP concentration to activate BMP signaling by enhanced basal phosphorylation levels of Smad 1/5/8. In addition, the significantly reduced expression levels of CD146 and CD90 with the presence of Noggin confirms the direct effect of BMP4 on the stem cell-like phenotype of genetically modified PF cells (BT-PFs). Furthermore, BT-PFs exhibited a high neural differentiation capacity (475%). After transplantation into NOD/SCID mice, genetically modified-PFs generated cementum/PDL-like structures on the surface of the carrier. The multipotency of these modified cells potentially provides an attractive source of stem cells for therapeutic purposes and regenerative medicine.

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Section: Differentiation Of Periodontal Ligament Fibroblasts H-w MI Ementioning

confidence: 69%

Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer

et al. 2011

Gene Ther

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“…Previous reports have demonstrated that xenogenic bone graft and growth factors, including transforming growth factor-beta (TGF-b), bone morphogenetic protein (BMP)-2, BMP-4, and platelet-derived growth factor (PDGF), improve periodontal tissue regeneration. [41][42][43][44] MSC transplantation can supply large numbers of necessary cells and increase periodontal regenerative capacity. 45,46 It also well known that MSCs can differentiate into the desired tissues, such as cementum, alveolar bone, dentin, and collagen fibers.…”

Section: Discussionmentioning

confidence: 99%

Recombinant Human Plasminogen Activator Inhibitor-1 Promotes Cementogenic Differentiation of Human Periodontal Ligament Stem Cells

Hexiu

Choung

Lim

et al. 2015

Tissue Engineering Part A

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“…The barrier membrane prevents unwanted epithelium and gingival connective tissue from entering the healing site, while promoting repopulation of the defect site by cells migrating in from the PDL (29). The rather limited success of this approach has led scientists to develop methods to improve this therapy, through the addition of exogenous growth factors and via stem cell therapy (38). One goal of current research is to use different populations of dental stem cells to replicate the key events in periodontal development both temporally and spatially, so that healing can occur in a sequential manner to regenerate the periodontium (39).…”

Section: Dental Pulp Stem Cells and Dental Tissue Engineeringmentioning

confidence: 99%

Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration

et al. 2011

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In recent years, stem cell research has grown exponentially owing to the recognition that stem cell-based therapies have the potential to improve the life of patients with conditions that range from Alzheimer’s disease to cardiac ischemia and regenerative medicine, like bone or tooth loss. Based on their ability to rescue and/or repair injured tissue and partially restore organ function, multiple types of stem/progenitor cells have been speculated. Growing evidence demonstrates that stem cells are primarily found in niches and that certain tissues contain more stem cells than others. Among these tissues, the dental tissues are considered a rich source of mesenchymal stem cells that are suitable for tissue engineering applications. It is known that these stem cells have the potential to differentiate into several cell types, including odontoblasts, neural progenitors, osteoblasts, chondrocytes, and adipocytes. In dentistry, stem cell biology and tissue engineering are of great interest since may provide an innovative for generation of clinical material and/or tissue regeneration. Mesenchymal stem cells were demonstrated in dental tissues, including dental pulp, periodontal ligament, dental papilla, and dental follicle. These stem cells can be isolated and grown under defined tissue culture conditions, and are potential cells for use in tissue engineering, including, dental tissue, nerves and bone regeneration. More recently, another source of stem cell has been successfully generated from human somatic cells into a pluripotent stage, the induced pluripotent stem cells (iPS cells), allowing creation of patient- and disease-specific stem cells. Collectively, the multipotency, high proliferation rates, and accessibility make the dental stem cell an attractive source of mesenchymal stem cells for tissue regeneration. This review describes new findings in the field of dental stem cell research and on their potential use in the tissue regeneration.

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Periodontal regeneration using ex vivo autologous stem cells engineered to express the BMP-2 gene: an alternative to alveolaplasty

Cited by 60 publications

References 44 publications

Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer

Highly efficient multipotent differentiation of human periodontal ligament fibroblasts induced by combined BMP4 and hTERT gene transfer

Recombinant Human Plasminogen Activator Inhibitor-1 Promotes Cementogenic Differentiation of Human Periodontal Ligament Stem Cells

Mesenchymal stem cells in the dental tissues: perspectives for tissue regeneration

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