Periodontal regeneration employing gingival margin‐derived stem/progenitor cells: an animal study

El‐Sayed, Karim M. Fawzy; Paris, Sebastian; Becker, Stephan; Neuschl, Matthias; Buhr, Wiebke de; Sälzer, Sonja; Wulff, Andreas; El-Refai, Mahmoud I.; Darhous, Mona; Elmasry, Manal; Wiltfang, Jens; Dörfer, Christof E.

doi:10.1111/j.1600-051x.2012.01904.x

Cited by 85 publications

(98 citation statements)

References 46 publications

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“…A bone regenerative capacity of GMSCs has been confirmed by multiple studies, as these cells were able to repair the critical size mandibular/calvarial defects in animals [11][12][13]. Moreover, the transplanted GMSCs proved a significant periodontal regenerative potential in vivo, with newly formed alveolar bone, cementum, and periodontal ligament fibers [14][15][16].…”

Section: Introductionmentioning

confidence: 75%

Treatment of intrabony defects with modified perforated membranes in aggressive periodontitis: a 12-month randomized controlled trial

et al. 2018

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Objective The aim of this study was to compare the clinical and radiographic efficacy of guided tissue regeneration with a modified perforated collagen membrane (MPM) or standard collagen membrane (CM) in the treatment of intrabony defects in patients with aggressive periodontitis (AgP). Materials and methods Fifteen AgP patients were included in the study. Two single intrabony defects of at least 3 mm depth with ≥ 6 mm probing pocket depth (PPD) from each patient were randomly assigned to either xenogenic graft plus MPM (test group) or xenogenic graft plus CM (control group). PPD, clinical attachment level (CAL), and gingival recession (GR) were recorded at baseline and at 12 months. The radiographic assessments included the measurements of defect depth (DD), change in alveolar crest position (ACP), linear defect fill (LDF), and percentage defect fill (%DF). Results After treatment, PPD, CAL, DD, and ACP values improved significantly in both groups, without statistical differences between them. However, with respect to LDF and %DF, the 12-month radiographic analysis at MPM-treated sites showed a significant improvement compared to the 6-month outcomes, that was not observed at control sites (additional LDF of 0.4 ± 0.5 mm, p = 0.010 and %DF of 6.4 ± 7.6%, p = 0.025). Conclusions Both strategies proved effective in the treatment of intrabony defects in patients with AgP. Nonetheless, enhanced LDF and %DF 12 months postoperatively at MPM-treated sites may stem from cellular and molecular migration from the periosteum and overlying gingival connective tissue through barrier's pores. Clinical relevance Modification of CM may have positive ramifications on periodontal regeneration.

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Section: Introductionmentioning

confidence: 75%

Treatment of intrabony defects with modified perforated membranes in aggressive periodontitis: a 12-month randomized controlled trial

et al. 2018

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“…[62] . Likewise, Fawzy El-Sayed et al induced bilateral periodontal defects in the premolar and molar regions of a miniature pig model, showing that GMSC possessed the capability to regenerate bone, cementum and periodontal ligament [63] . Furthermore, Xu et al demonstrated that GMSC are able to migrate towards site of injury [64] .…”

Section: Resultsmentioning

confidence: 99%

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Nguyen

James

et al. 2015

International Journal of Orthopaedics

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Tissue specific mesenchymal stem cells are of great scientific interest across diverse fields in stem cell biology and medicine. Sources of mesenchymal stem cells (MSC) in the craniomaxillofacial skeleton are numerous and are of significant interest to craniofacial and oral surgeons, among other medical and dental specialties. Mesenchymal stem cells are defined by their characteristic cell surface marker expression, multipotentiality and ability for self-renewal. Bone marrow derived MSC (BMSC) are most commonly studied, but are of low abundance in the craniomaxillofacial (CMF) skeleton. Alternative CMF sources of MSC are diverse and include dental pulpal stem cells (DPSC), periodontal ligament stem cells (PDLSC), suture-derived mesenchymal stromal cells (SMC), gingival mesenchymal stem cells (GMSC)and adipose-derived MSC (ASC). This brief review will introduce various MSC sources derived from the head and neck, along with a discussion of their identity, characteristics and results of preclinical studies in tissue engineering.

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“…MSCs exhibiting these properties have now been isolated from a wide range of tissues, including bone marrow, adipose tissue, fetal liver and blood, Wharton's Jelly of the umbilical cord, and dental tissues, including periodontal ligament (PDL MSC), dental pulp (DPSC), exfoliated deciduous teeth (SHED cells), and dental follicle [4,[19][20][21]. Recent studies have also described the isolation and characterization of MSCs from healthy and inflamed gingival tissue by isolation of clonogenic cells or magnet immunosorting, and this has been proposed as a potentially more abundant source of tissue for deriving autologous MSCs when compared to PDL [22][23][24]. A recent study also suggested that gingiva-derived MSCs include both neural crest-derived and mesodermally derived cells [25•].…”

Section: Mesenchymal Stem Cellsmentioning

confidence: 99%

“…A range of different cell sources, including PDL MSC [54••], DPSC [55], adipose tissue [52], BMSC [57], and gingival MSC, have demonstrated the ability to increase regeneration of periodontal tissues [24]. Although these studies have not demonstrated superior results of any particular cell type over another, PDL cells were found to have superior outcomes in a study by Park and colleagues that tested the effects of autologous PDL, DPSC, and follicular stem cells on regeneration of defects extending to the root apex in a canine model [58].…”

Section: Use Of Msc For Periodontal Regeneration In Animal Modelsmentioning

confidence: 99%

Mesenchymal Stem Cells and Periodontal Regeneration

Hughes

2014

Curr Oral Health Rep

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The use of mesenchymal stem cell (MSC) therapy offers the potential to develop a completely novel and improved method of periodontal regeneration compared to existing methods. Since the initial demonstration of the presence of MSCs in the periodontal ligament, many recent studies have now demonstrated the potential for the transplantation of MSCs from PDL and other sources to enhance periodontal regenerative outcomes in a variety of animal models. In addition, the recent demonstration of the possible utility of allogeneic MSCs and MSCs derived from inducible pluripotent stem cells may offer new methods of delivering such therapies. Determination of the specific roles that MSCs may play in enhancing regenerative outcomes requires further investigation. The principle of MSC-directed periodontal regenerative therapy is accepted in the field, but extensive investigation is necessary to establish viable, efficacious, and practically applicable human therapies.

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Periodontal regeneration employing gingival margin‐derived stem/progenitor cells: an animal study

Abstract: Gingival margin-derived stem/progenitor cells show significant periodontal regenerative potential.

Cited by 85 publications

References 46 publications

Treatment of intrabony defects with modified perforated membranes in aggressive periodontitis: a 12-month randomized controlled trial

Treatment of intrabony defects with modified perforated membranes in aggressive periodontitis: a 12-month randomized controlled trial

Craniomaxillofacial Sources of Mesenchymal Stem Cells: A Brief Review

Mesenchymal Stem Cells and Periodontal Regeneration

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