Periodontal ligament stem/progenitor cells with protein-releasing scaffolds for cementum formation and integration on dentin surface

Cho, Hankyu; Tarafder, Solaiman; Fogge, Michael; Kao, Kristy; Lee, Chang H.

doi:10.1080/03008207.2016.1191478

Cited by 52 publications

(40 citation statements)

References 35 publications

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“…PDLSCs are important for the homeostasis of the periodontium. These stem cells are regarded not only as putative candidates for dental tissue engineering [ 4 , 5 ], but also as a potential resource to differentiate into bone-forming osteoblasts [ 6 ], which could be useful for the reconstruction of damaged periodontium and bone tissues. Furthermore, PDLSCs can be easily collected from baby teeth and wisdom teeth, unlike harvesting bone marrow-derived stem cells by invasive surgery or cord blood stem cells at birth.…”

Section: Introductionmentioning

confidence: 99%

Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

et al. 2017

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BackgroundOxysterols, oxygenated by-products of cholesterol biosynthesis, play roles in various physiological and pathological systems. However, the effects of oxysterols on periodontal regeneration are unknown. This study investigated the effects of the specific oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol (SS) on the regeneration of periodontal tissues using in-vitro periodontal ligament stem cells (PDLSCs) and in-vivo models of alveolar bone defect.MethodsTo evaluate the effects of the combined oxysterols on PDLSC biology, we studied the SS-induced osteogenic differentiation of PDLSCs by assessing alkaline phosphatase activity, intracellular calcium levels [Ca2+]i, matrix mineralization, and osteogenic marker mRNA expression and protein levels. To verify the effect of oxysterols on alveolar bone regeneration, we employed tooth extraction bone defect models.ResultsOxysterols increased the osteogenic activity of PDLSCs compared with the control group. The expression of liver X receptor (LXR) α and β, the nuclear receptors for oxysterols, and their target gene, ATP-binding cassette transporter A1 (ABCA1), increased significantly during osteogenesis. Oxysterols also increased protein levels of the hedgehog (Hh) receptor Smo and the transcription factor Gli1. We further confirmed the reciprocal reaction between the LXRs and Hh signaling. Transfection of both LXRα and LXRβ siRNAs decreased Smo and Gli1 protein levels. In contrast, the inhibition of Hh signaling attenuated the LXRα and LXRβ protein levels. Subsequently, SS-induced osteogenic activity of PDLSCs was suppressed by the inhibition of LXRs or Hh signaling. The application of SS also enhanced bone formation in the defect sites of in-vivo models, showing equivalent efficacy to recombinant human bone morphogenetic protein-2.ConclusionsThese findings suggest that a specific combination of oxysterols promoted periodontal regeneration by regulating PDLSC activity and alveolar bone regeneration.Electronic supplementary materialThe online version of this article (doi:10.1186/s13287-017-0725-9) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

et al. 2017

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“…After 6 weeks of culturing, they found that cementumlike layer can be successfully formed in this interface between cementum and human PDL like tissue. They also found that BMP7 helped in cementum matrix protein 1 secretion in vitro, which may be good for cementum tissue establishment (Cho et al, 2016b).…”

Section: Combined Dental Tissue Regenerationmentioning

confidence: 95%

“…Dental tissue includes composites of enamel, dentin and pulp, periodontal ligament, cementum, and so on. Since the dental tissue are related with each other, some researches chose to establish combined dental tissue like scaffolds with AM technology, such as cementum/dentin interface (Lee et al, 2014a) or cementum/PDL interface (Cho et al, 2016a). Various materials can be used in AM technology for dental tissue (Table 3).…”

Section: Technique Challenges For Cartilage Printing and Current Stramentioning

confidence: 99%

Peer Review #3 of "Three-dimensional printing with biomaterials in craniofacial and dental tissue engineering (v0.1)"

2019

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With the development of technology, tissue engineering (TE) has been widely applied in medical field. In recent years, due to its accuracy and demands of solid freeform fabrication in TE, three-dimensional printing, also known as additive manufacturing (AM), has been applied for biological scaffolds fabrication in craniofacial and dental regeneration. In this review, we have compared several types of AM techniques and summarized their advantages and limitations. The range of printable materials used in craniofacial and dental tissue includes all the biomaterials. Thus, basic and clinical studies were discussed in this review to present the application of AM techniques in craniofacial and dental tissue and their advances during these years, which might provide information for further AM studies in craniofacial and dental TE.

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“…PDLSCs are heterogeneous cells that participate in maintaining periodontium homeostasis, whereas in vitro , they have the ability to differentiate into chondrocytes, adipocytes, osteoblast, neural cells, and hepatocytes (Seo et al, 2004 ; Bartold et al, 2006 ). Additionally, it was found that CD146 + cells isolated from PDLSCs display better clonogenicity, multipotency, and regenerative potential than heterogeneous PDLSCs, suggesting that a specific population of PDLSCs might help in effectively combating periodontitis (Seo et al, 2004 ; Cho et al, 2016 ).…”

Section: Multipotent Differentiation Of Human Dental Tissue-derived Mmentioning

confidence: 99%

Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth

et al. 2017

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Adult stem cells have attracted scientific attention because they are able to self-renew and differentiate into several specialized cell types. In this context, human dental tissue-derived mesenchymal stem cells (hDT-MSCs) have emerged as a possible solution for repairing or regenerating damaged tissues. These cells can be isolated from primary teeth that are naturally replaced, third molars, or other dental tissues and exhibit self-renewal, a high proliferative rate and a great multilineage potential. However, the cellular and molecular mechanisms that determine lineage specification are still largely unknown. It is known that a change in cell fate requires the deletion of existing transcriptional programs, followed by the establishment of a new developmental program to give rise to a new cell lineage. Increasing evidence indicates that chromatin structure conformation can influence cell fate. In this way, reversible chemical modifications at the DNA or histone level, and combinations thereof can activate or inactivate cell-type-specific gene sequences, giving rise to an alternative cell fates. On the other hand, miRNAs are starting to emerge as a possible player in establishing particular somatic lineages. In this review, we discuss two new and promising research fields in medicine and biology, epigenetics and stem cells, by summarizing the properties of hDT-MSCs and highlighting the recent findings on epigenetic contributions to the regulation of cellular differentiation.

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Periodontal ligament stem/progenitor cells with protein-releasing scaffolds for cementum formation and integration on dentin surface

Abstract: Our findings represent important progress in stem cell-based cementum regeneration as an essential part of periodontium regeneration.

Cited by 52 publications

References 35 publications

Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

Peer Review #3 of "Three-dimensional printing with biomaterials in craniofacial and dental tissue engineering (v0.1)"

Stem Cells from Dental Pulp: What Epigenetics Can Do with Your Tooth

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