Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

Lee, Jinsun; Kim, Eunji; Han, Seong‐Gu; Kang, Kyung Lhi; Heo, Jung Sun

doi:10.1186/s13287-017-0725-9

Cited by 25 publications

(33 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…All fourteen excluded studies were eliminated for lack of full-text availability. Of the 13 included studies – all preclinical – five employed spinal fusion models[ 19 - 21 , 31 , 33 ] (Table 1 ), two employed critical-sized alveolar defect models[ 35 , 37 ] (Table 2 ), and six employed critical-sized calvarial defect models[ 17 , 30 , 32 , 34 , 36 , 38 ] (Table 3 ). Across all studies there were 7 unique oxysterols evaluated (Table 4 ).…”

Section: Resultsmentioning

confidence: 99%

“…Table 2 contains descriptive summaries of the 2 studies examining oxysterols in critical-sized alveolar defect models, both of which employed rat models. Lee et al[ 35 ] employed a two-site defect model and injected solutions of DMSO, rhBMP-2, or a combination of 20( S )-hydroxycholesterol and 22( S )-hydroxycholesterol into the extracted sockets. These treatments produced mean bone regeneration of 45%, 53%, and 65%, respectively[ 35 ].…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Oxysterols as promising small molecules for bone tissue engineering: Systematic review

Cottrill

Lazzari

Pennington

et al. 2020

WJO

View full text Add to dashboard Cite

BACKGROUND Bone tissue engineering is an area of continued interest within orthopaedic surgery, as it promises to create implantable bone substitute materials that obviate the need for autologous bone graft. Recently, oxysterols – oxygenated derivatives of cholesterol – have been proposed as a novel class of osteoinductive small molecules for bone tissue engineering. Here, we present the first systematic review of the in vivo evidence describing the potential therapeutic utility of oxysterols for bone tissue engineering. AIM To systematically review the available literature examining the effect of oxysterols on in vivo bone formation. METHODS We conducted a systematic review of the literature following PRISMA guidelines. Using the PubMed/MEDLINE, Embase, and Web of Science databases, we queried all publications in the English-language literature investigating the effect of oxysterols on in vivo bone formation. Articles were screened for eligibility using PICOS criteria and assessed for potential bias using an expanded version of the SYRCLE Risk of Bias assessment tool. All full-text articles examining the effect of oxysterols on in vivo bone formation were included. Extracted data included: Animal species, surgical/defect model, description of therapeutic and control treatments, and method for assessing bone growth. Primary outcome was fusion rate for spinal fusion models and percent bone regeneration for critical-sized defect models. Data were tabulated and described by both surgical/defect model and oxysterol employed. Additionally, data from all included studies were aggregated to posit the mechanism by which oxysterols may mediate in vivo bone formation. RESULTS Our search identified 267 unique articles, of which 27 underwent full-text review. Thirteen studies (all preclinical) met our inclusion/exclusion criteria. Of the 13 included studies, 5 employed spinal fusion models, 2 employed critical-sized alveolar defect models, and 6 employed critical-sized calvarial defect models. Based upon SYRCLE criteria, the included studies were found to possess an overall “unclear risk of bias”; 54% of studies reported treatment randomization and 38% reported blinding at any level. Overall, seven unique oxysterols were evaluated: 20( S )-hydroxycholesterol, 22( R )-hydroxycholesterol, 22( S )-hydroxycholesterol, Oxy4/Oxy34, Oxy18, Oxy21/Oxy133, and Oxy49. All had statistically significant in vivo osteoinductive properties, with Oxy4/Oxy34, Oxy21/Oxy133, and Oxy49 showing a dose-dependent effect in some cases. In the eight studies that directly compared oxysterols to rhBMP-2-treated animals, similar rates of bone growth occurred in the two groups. Biochemical investigation of these ...

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Oxysterols as promising small molecules for bone tissue engineering: Systematic review

Cottrill

Lazzari

Pennington

et al. 2020

WJO

View full text Add to dashboard Cite

show abstract

“…In the same way, ABCA1, a cAMP-dependent and sulfonylurea-sensitive anion transporter present in the endosome and plasma membrane also depicted a potential link with APOE that mediates the binding, internalization, and catabolism of lipoprotein particles. A recent study showed that oxysterols increased the osteogenic activity of PDLSCs and the expression of ABCA1, increased significantly during osteogenesis [98]. Further, APOE, in particular its isoform 4, was recently proposed to increase the risk of periodontitis [99] and APOE-2 allele is associated with higher prevalence of sporadic PD [100,101].…”

Section: Discussionmentioning

confidence: 99%

Network Protein Interaction in Parkinson's Disease and Periodontitis Interplay: A Bioinformatic Analysis

Botelho¹,

Mascarenhas²,

Mendes³

et al. 2020

Preprint

View full text Add to dashboard Cite

Recent studies supported a clinical association between Parkinson’s Disease (PD) and periodontitis. Hence, investigating possible protein interactions between these two conditions is of interest. In this study, we conducted a protein-protein network interaction analysis with recognized genes encoding proteins for PD and periodontitis. Genes of interest were collected via GWAS database. Then, we conducted a protein interaction analysis using STRING database, with a highest confidence cut-off of 0.9. Our protein network casted a comprehensive analysis of potential protein-protein interactions between PD and periodontitis. This analysis may underpin valuable information for new candidate molecular mechanisms between PD and periodontitis and may serve new potential targets for research purposes. These results should be carefully interpreted giving the limitations of this approach.

show abstract

“…All subjects involved in this study were informed about its purpose and procedures, and the study was approved by the Review Board of Kyung Hee University. PDLs were collected from the middle thirds of roots and cultured in minimal essential medium (α-MEM; Invitrogen) containing 10% FBS, penicillin (100 U/mL), and streptomycin (100 g/mL) according to a previously described method (Kim et al, 2013;Lee et al, 2017b). All experiments were carried out with passage 4-7 cells.…”

Section: Pdl Cell Culture and Treatmentmentioning

confidence: 99%

Titanium dioxide nanoparticles induce COX-2 expression through ROS generation in human periodontal ligament cells

et al. 2019

Self Cite

View full text Add to dashboard Cite

Titanium dioxide nanoparticles (TiO 2 -NPs) are used to improve the aesthetic of toothpaste. While TiO 2 -NPs have been used safely in toothpaste products for a long time, there haven't been studies to determine whether absorption of TiO 2 -NPs by the mucous membranes in the mouth induces pathogenic conditions. Here, we assessed whether TiO 2 -NPs induce cyclooxygenase-2 (COX-2) and investigated the molecular mechanisms underlying the pro-inflammatory effect of TiO 2 -NPs on human periodontal ligament (PDL) cells. Treatment of PDL cells with TiO 2 -NPs led to induction of both COX-2 mRNA and protein expression. TiO 2 -NPs stimulated the nuclear translocation of nuclear factor-kappaB (NF-κB) as well as its DNA binding by inducing phosphorylation and subsequent degradation of the inhibitory protein IκBα in PDL cells. TiO 2 -NPs treatment resulted in rapid activation of extracellular signal-regulated kinase (ERK)1/2 and Akt, which could be upstream of NF-κB. Treatment of PDL cells with both the MEK1/2 inhibitor U0126 and the PI3K inhibitor LY294002 strongly attenuated TiO 2 -NPs-induced activation of NF-κB, and also the expression of COX-2. PDL cells treated with TiO 2 -NPs exhibited increased accumulation of intracellular reactive oxygen species (ROS). Pretreatment of cells with ROS scavenger N-acetyl cysteine (NAC) abrogated the stimulatory effect of TiO 2 -NPs on p65, p50, and COX-2 expression. In conclusion, ROS, concomitantly overproduced by TiO 2 -NPs, induce COX-2 expression through activation of NF-κB signaling, which may contribute to the inflammatory effect of PDL cells.

show abstract

Evaluating the oxysterol combination of 22(S)-hydroxycholesterol and 20(S)-hydroxycholesterol in periodontal regeneration using periodontal ligament stem cells and alveolar bone healing models

Cited by 25 publications

References 35 publications

Oxysterols as promising small molecules for bone tissue engineering: Systematic review

Oxysterols as promising small molecules for bone tissue engineering: Systematic review

Network Protein Interaction in Parkinson's Disease and Periodontitis Interplay: A Bioinformatic Analysis

Titanium dioxide nanoparticles induce COX-2 expression through ROS generation in human periodontal ligament cells

Contact Info

Product

Resources

About