Periodontal healing of replanted dog teeth stored in milk and egg albumen

Abstract

The type of storage medium used to store avulsed teeth prior to replantation has been shown to be a decisive factor in periodontal ligament (PDL) healing. The aim of the present study was to investigate the effect of storage medium on periodontal healing. Thirty teeth from three dogs were endodontically treated to prevent subsequent inflammatory root resorption. The teeth were atraumatically extracted and randomly stored in milk or egg albumen for 3, 6 and 10 h at 4 degrees C. All teeth were splinted for 1 weeks after replanting. After 2 months animals were sacrificed using vital perfusion-fixation and teeth were histologically prepared and evaluated following Andreasen's method. It was found that teeth stored in egg albumen for 6 and 10 h had significantly higher incident of PDL healing than those treated with milk for the same period (P < 0.05). . The highest incidence of PDL healing was observed in teeth stored in egg albumen for 6 h. The least surface resorption was also evident in this group (P < 0.05). The result of this study shows that egg albumen is an excellent storage media for up to 10 h considering its likely availability at most accident sites.

Egg White-Milk Mixture as a Novel Biological Storage Media for Avulsed teeth Periodontal Ligament Cells: An In-Vitro Study

“…They were promising concerning the organization of collagen fibres and the number of viable cells. Some suggest that they can be perfect media for storing avulsed teeth but they need additional studies [4,6,[18][19][20][21]. Some considered milk as a gold standard for transporting avulsed teeth.…”

“…However, other studies demonstrated that egg white reported higher viable PDL cells than milk after half hour storage time [25,26]. On the same context Khademi., et al found that teeth stored in egg white for 6 to 10h had a better incidence of repair and lower surface resorption than those stored in milk [20]. According to the International Association of Dental Traumatology and the American Academy of Pediatric Dentistry milk is considered as the second or third best transportation media for avulsed teeth include after Viaspan and/or HBSS [23,27].…”

“…The extracted teeth (90) were equally divided and stored immediately post-extraction in HBSS media and egg-white-milk mixture media [20,24]. These teeth were sub-classified according storage media and time of storage into 6 equal groups (each group was formed of 15 teeth) as following:…”

“…The viable and non-viable cells were counted using digital microscope with magnification 20X with a corresponding special software in area 121 x 129 Mm for each slide in General Pathology Lab. of National Research Centre (Figure 1) [19][20][21]. …”

Current Developments in Transport Media for Avulsed Teeth: An Update

“…The osmolality of the egg white is between 251 and 298 mOsm/kg [24]. de Sousa et al have microscopically analyzed, the human PDL attached to the extracted tooth after 1 hr of extra alveolar time, compared to milk, egg white, and artificial saliva.…”