Periodic Acid-Schiff Staining of Retinal Whole Mounts

Engerman, Ronald L.; Buesseler, John A.; Meyer, Roland K.

doi:10.1001/archopht.1962.00960030066013

Cited by 12 publications

(3 citation statements)

References 5 publications

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“…Retinas were examined histologically as wholemounts stained with PAS according to the method of Engerman, Buesseler and Meyer (1962). In successful preparations the vessels could be correlated with their photographed appearance in life and the whole vasculature, including capillaries, its topography relatively intact, could be followed in detail.…”

Section: Methodsmentioning

confidence: 99%

Retinal Arterioles in the New Zealand Strain of Genetically Hypertensive Rats

Parr

1976

Aust N Z J Ophthal

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Section: Methodsmentioning

confidence: 99%

Retinal Arterioles in the New Zealand Strain of Genetically Hypertensive Rats

Parr

1976

Aust N Z J Ophthal

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“…The original method proposed by Engerman et al in 1962 did not provide satisfactory results by reason of the marked coloration of the background, although of the recommended fixation in Heidenhein's SUSA fixative was carried out. When the retinas were incubated in 0.05 M phosphate buffer (pH 7.0) containing 0.3 O/O a-amylase before staining, the background staining was less, although the vascular details were not revealed with sufficient accuracy.…”

mentioning

confidence: 99%

“…The technique described by Engerman et al (1962Engerman et al ( , 1965 was applied for flat mounts of whole retinas and the method of Kuwabara and Cogan (1960), with slight modifications, for digested preparations of retinal vessels, as described later. Cross sections were made of both paraffin embedded and frozen specimens.…”

mentioning

confidence: 99%

Vascular Structures of the Rabbit Retina*

Hyvdrinen

1967

Acta Ophthalmologica

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Several authors have described the vascular structures of the rabbit retina and its circulation. Michaelson (1954) was concerned with the preretinal character of the vascular net, and the partial vascularization of the retina by the retinal vessels. Mutlu and Leopold (1964) were interested in the structures of the capillary wall, and Ruskell (1962), in his study of the orbital arteries, described the different origins of the retinal artery. The most extensive study of the retinal vasculature in the rabbit has been that made by Rohen (1954), whose publication contains a review of earlier literature.During studies of the circulation in rabbit retinal vessels (Hyvarinen 1967 b), it was found necessary first to study their anatomy in detail. Flat preparation of the retinal whole mounts and digested vessel nets was decided upon for visualization of the three-dimensional structure of the vascular net, and for study of the connections between the superficial and deep vessels and the cellular structures of the capillary walls. The application of these new techniques, combined with serial paraffin cross-sections, made accurate study of the details of the retinal vessels possible, with the result that some new features could be revealed in the vasculature of the rabbit retina. MATERIAL A N D METHODSExaminations were made of 46 eyes of 30 healthy rabbits, both albino and grey, weighing from 2.5 kg to 5 kg, and varying in age from 6 to 30 months. The 'animals were killed by intravenous injections of 5 cc sodium pentobarbital. The eyes were enucleated immediately after death, and fixed in 10 O/O neutral formalin or Heidenhein's SUSA fixative.The retinal vessels were studied both in flat mounts and cross sections.

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Experimental Diabetic Retinopathy in Dogs

Engerman¹,

Bloodworth²

1965

Archives of Ophthalmology

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Periodic Acid-Schiff Staining of Retinal Whole Mounts

Cited by 12 publications

References 5 publications

Retinal Arterioles in the New Zealand Strain of Genetically Hypertensive Rats

Retinal Arterioles in the New Zealand Strain of Genetically Hypertensive Rats

Vascular Structures of the Rabbit Retina*

Experimental Diabetic Retinopathy in Dogs

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