1980
DOI: 10.1016/s0008-6215(00)85321-5
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Periodate oxidation and alkaline degradation of heparin-related glycans

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Cited by 49 publications
(32 citation statements)
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“…In the case of heparin and highly sulphated oligosaccharides the reaction mixtures contained 0.2 M sodium perchlorate in order to minimize periodatepolyanion repulsion [28]. Oxidation at pH 3.0 and 4 "C selectively destroys GlcUA in GlcNAc-containing block regions [15,16]. The extent of oxidation under the three different conditions (a -c) were essentially the same [16] despite a recent claim that citrate is consumed by periodate (J. E. Scott, personal communication).…”
Section: Degradative Methodsmentioning
confidence: 99%
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“…In the case of heparin and highly sulphated oligosaccharides the reaction mixtures contained 0.2 M sodium perchlorate in order to minimize periodatepolyanion repulsion [28]. Oxidation at pH 3.0 and 4 "C selectively destroys GlcUA in GlcNAc-containing block regions [15,16]. The extent of oxidation under the three different conditions (a -c) were essentially the same [16] despite a recent claim that citrate is consumed by periodate (J. E. Scott, personal communication).…”
Section: Degradative Methodsmentioning
confidence: 99%
“…Oxidation at pH 3.0 and 4 "C selectively destroys GlcUA in GlcNAc-containing block regions [15,16]. The extent of oxidation under the three different conditions (a -c) were essentially the same [16] despite a recent claim that citrate is consumed by periodate (J. E. Scott, personal communication). At pH 7.0 and 37 "C all non-sulphated uronic acids are oxidised.…”
Section: Degradative Methodsmentioning
confidence: 99%
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