Perinotochordal connective sheet of gilthead sea bream larvae (<i>Sparus aurata</i>, L.) affected by axial malformations: An histochemical and immunocytochemical study

Sanatamaría, J. A.; Andrades, José A.; Herráez, M.P.; Fernández-Llébrez, Pedro; Becerra, José

doi:10.1002/ar.1092400212

Cited by 22 publications

(18 citation statements)

References 29 publications

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“…For instance, some authors have reported that vertebral deformities may appear during the notochord segmentation and vertebral centrum differentiation processes (Fernández et al 2008, Haga et al 2009). Others have suggested that they are a consequence of dysfunctions in collagen metabolism at notochordal and perinotochordal collagen sheets during early development (Sanatamaría et al 1994). Moreover, vertebral deformities may also occur later in ontogeny (i.e.…”

Section: Discussionmentioning

confidence: 99%

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Ortiz-Delgado¹,

Fernández²,

Sarasquete³

et al. 2014

Aquat. Biol.

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During the last decades, the finfish larviculture industry has considerably improved its rearing methods under intensive conditions, but larval quality remains one of the main problems for the proper sustainable success of this productive sector (Kou moundouros 2010, Boglione et al. 2013a). The quality of farmed fish is directly related to the quality of the fry, and depends on both organoleptic and morphological characteristics that should be as similar as possible to that of wild fish, which is considered to be the ABSTRACT: This study provides a comprehensive description of the tissue organization of nondeformed and deformed opercula and vertebrae from gilthead sea bream Sparus aurata juveniles by means of histological, histochemical and immunohistochemical approaches. Two types of opercular anomalies are described: the folding of the opercle and subopercle into the gill chamber, starting at the upper corner of the branchial cleft and extending down to its lower third; and the partial lack of the operculum (opercle, subopercle, interopercle and preopercle underdeveloped) with a regression of the loose edge extending down to its lower third. Histological observations revealed a rare type of bone remodelling process in the opercular structure, which consisted of the coalescence of contacting bone tissues (presumably from the preopercle and opercle), resulting in skeletal tissue with a trabecular aspect filled by a single-cell epithelium of cubic osteoblastic-like cells. Differences in collagen fiber thickness and its 3-dimensional arrangement between normal and deformed opercula were also found. Lordotic vertebrae were characterized by the formation of fibrous cartilage in the haemal and/or neural sides, indicating that a metaplastic shift occurred during the process of lordosis. Another major histomorphological change found in lordotic vertebrae was the complete loss of notochordal sheath integrity. Histological alterations were coupled with an imbalance of cell death and cell proliferation processes in lordotic vertebrae as well as that of bone formation/resorption, and extracellular matrix deposition activity differences which might have resulted from the remodelling process occurring in lordotic vertebrae. Altogether, these results provide an increase in our basic knowledge of bone disorders that contribute to our understanding of the mechanisms by which these skeletal anomalies appear in this fish species and which hamper its production efficiency.

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Section: Discussionmentioning

confidence: 99%

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Ortiz-Delgado¹,

Fernández²,

Sarasquete³

et al. 2014

Aquat. Biol.

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“…The first effect is buckling failure of the axial skeleton due to an increase in compressive load. Buckling of the axial skeleton has been reported prior to vertebrae formation in sea bream (Sparus aurata) (Andrades et al, 1996;Santamarı´a et al, 1994), but is more commonly correlated with the onset of bony vertebrae formation (Kihara et al, 2002). Interestingly, buckling of the vertebral column in animals without an inflated swim bladder occurs more anteriorly than in fish with an inflated swim bladder (Divanach et al, 1997;Chatain, 1994).…”

Section: Article In Pressmentioning

confidence: 94%

Lordotic vertebrae in sea bass (Dicentrarchus labrax L.) are adapted to increased loads

Kranenbarg

Waarsing

Müller

et al. 2005

Journal of Biomechanics

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“…7.1 and 7.2). This tissue is composed by an acellular, fibrous elastic sheath, covering a glycosaminoglycan-rich collagenous layer (Grotmol et al, 2005;Sanatamaría et al, 2005), that encloses and constrains the net of chordocytes in the inner core, surrounded by an outer layer of chordoblasts. Using HE, the chordoblast nucleus is stained by haematoxylin while the notochord is clearly stained with eosin, (Fig.…”

Section: The Vertebrae Of Turbotmentioning

confidence: 99%

Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation

et al. 2009

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Matrix Gla protein (Mgp) is a secreted vitamin K-dependent extracellular matrix protein and a physiological inhibitor of calcification whose gene structure, amino acid sequence and tissue distribution have been conserved throughout evolution. In the present work, the turbot (Scophthalmus maximus) mgp cDNA was cloned and the sequence of the deduced protein compared to that of other vertebrates. As expected, it was closer to teleosts than to other vertebrate groups but there was a strict conservation of amino-acids thought to be important for protein function. Analysis of mgp gene expression indicated branchial arches as the site with higher levels of expression, followed by heart, vertebra and kidney. These results were confirmed by in situ hybridization with a strong mgp expression in branchial arch chondrocytes. Mgp was found to accumulate in gills where it appeared to be restricted to chondrocytes from branchial filaments, while in vertebrae it was localized in vertebral end plates, in growth zones, in vertebral arches and spines and in notochord cells. In the soft tissues analysed, Mgp was mainly detected in kidney and heart, consistent with previous data and providing further evidence for a role of Mgp as a calcification inhibitor and a modulator of the mineralization process. Our studies provide evidence that turbot, an important new species for aquaculture, is also a useful model to study function and expression of Mgp.

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Perinotochordal connective sheet of gilthead sea bream larvae (Sparus aurata, L.) affected by axial malformations: An histochemical and immunocytochemical study

Abstract: Lordosis in Sparus aurata originated during embryonic development and was characterized by disorganized connective tissue and muscle bundles. No major differences in connective tissue constituents were seen with respect to normal specimens.

Cited by 22 publications

References 29 publications

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Normal and histopathological organization of the opercular bone and vertebrae in gilthead sea bream Sparus aurata

Lordotic vertebrae in sea bass (Dicentrarchus labrax L.) are adapted to increased loads

Matrix Gla protein in turbot (Scophthalmus maximus): Gene expression analysis and identification of sites of protein accumulation

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