Perilla frutescens Sprout Extracts Protected Against Cytokine-induced Cell Damage of Pancreatic RINm5F Cells via NF-κB Pathway

Abstract

Perilla frutescens (L.) Britton var. sprouts (PFS) is a plant of the labiatae family. The purpose of this work was to assess the preventive effects of PFS ethanolic extracts (PFSEs) on cytokine-induced β-cell damage. Cytokines, which are released by the infiltration of inflammatory cells around the pancreatic islets, are involved in the pathogenesis of type 1 diabetes mellitus. The combination of interleukin-1β (IL-1), interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) induced formation of reactive oxygen species (ROS). Accumulation of intracellular ROS led to β-cell dysfunction and apoptosis. PFSEs possess antioxidant activity and thus lead to downregulation of ROS generation. Cytokines decrease cell viability, stimulate the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and induce the production of nitric oxide (NO). PFSEs prevented cytokine-induced cell viability in a dose-dependent manner. Incubation with PFSE resulted in significant reduction in cytokine-induced NO production that correlated with reduced levels of the iNOS and COX-2 protein expression. Furthermore, PFSE significantly decreased the activation of nuclear factor κB (NF-κB) by inhibition of IκBα phosphorylation in RINm5F cells. In summary, our results suggest that the protective effects of PFSE might serve to counteract cytokine-induced β-cell destruction. Findings indicate that consumption of Perilla frutescens (L.) Britton var. sprouts alleviates hyperglycemia-mediated oxidative stress and pro-inflammatory cytokine-induced β-cell damage and thus has beneficial anti-diabetic effects.Key words : Cytokines, diabetes, NF-κB, Perilla frutescents, RINm5F

Perilla frutescens Sprout Extract Protect Renal Mesangial Cell Dysfunction against High Glucose by Modulating AMPK and NADPH Oxidase Signaling

“…This plant is commonly known as “Dlggae” in Korea [13]. Previously, we reported the antioxidant and hypoglycemic effects of the PF sprout extract (PFSE) in pancreatic β-cells and type 2 diabetic animal model [13,14]. However, the protective effect of the PFSE against DN and the underlying mechanism remains elusive.…”