Periacinar clefting and p63 immunostaining in prostatic intraepithelial neoplasia and prostatic carcinoma

Abstract

The aim of the present study was to correlate the presence and extent of retraction clefting and the expression of p63 in neoplastic glands and glands with prostatic intraepithelial neoplasia (PIN) in needle core biopsies.We analyzed needle core biopsies from 28 patients with PIN and 41 patients with adenocarcinoma. Neoplastic glands and those with PIN were analyzed on high power field (400x) and classified in three groups. Immunohistochemical staining was performed following Microwave Streptavidin ImmunoPeroxidase (MSIP) protocol on DAKO TechMate Horizon automated immunostainer using antibodies to p63 (4A4, M7247, DAKO, Denmark).Periacinar retraction clefting was significantly more prominent in prostatic carcinoma compared to PIN (p<0.0001) and nonneoplastic glands (p<0.0001). There was no difference between normal glands and PIN regarding clefting (p=0.8064). P63 was positive around whole circumference in 12 out of 28 cases with PIN, and discontinuously positive in remaining 16 PIN cases suggesting initial disruption of basal cell layer. P63 immunostaining was also positive in all nonneoplastic glands, and negative in all carcinoma cases.We conclude that retraction clefting was associated with cancer and lack of basal cells, but not with PIN. The relationship between clefting and p63 immunostaining in prostatic cancer should be further analyzed.

Periacinar Retraction Clefting and D2-40 Expression in Prostatic Adenocarcinoma

“…We found that retractions are significantly more prominent in prostatic carcinoma, compared to PIN and non-neoplastic glands, in which they only occur sporadically [6,19]. These findings are consistent with previous assumption that clefting is associated with lack of basal cells and stromal changes.…”

“…In our previous study, we analyzed periacinar retractions in p63 immunostained material of prostatic intraepithelial neoplasia (PIN) and prostatic carcinoma [19]. We found that retractions are significantly more prominent in prostatic carcinoma, compared to PIN and non-neoplastic glands, in which they only occur sporadically [6,19].…”

Significance of periacinar cleftings as supporting criteria in diagnosis of prostatic adenocarcinoma Gleason score-a 7 (3+4) and Gleason score-a 7 (4+3) and their relationship with parameters of predictive value

“…Dobijeni podaci idu u prilog činjenice da se periacinusne pukotine zaista mogu shvatiti kao važan pomoćni parametar u dijagnozi karcinoma prostate, za šta potvrda postoji i u literaturi [12,13,14,15,16,30]. …”

“…U novijoj literaturi kao važan pomoćni dijagnostički kriterijum pominju se i periacinusne retrakcione pukotine [12,13,14,15,16,22,30].…”

“…periacinusne retrakcione pukotine (eng. : periacinar retraction cleftings) [12,13,14,15,16,30]. Periacinusne pukotine predstavljaju "prazne prostore" ili haloe oko žlezda i mogu se smatrati za pomoćni dijagnostički kriterijum ako su prisutne u više od polovine žlezda i ako pri tome zahavataju najmanje 1/2, a sigurnije 2/3 cirkumferencije žlezde [15,31].…”