Perfusion of the cervical spinal cord in situ of adult rats using a perfluorocarbon emulsion

Perfluorochemical Perfusion of the Isolated Guinea Pig Heart

“…2). This low flow rate was not the result of a different viscosity: the viscosity at 37 °C of the PFC emulsion (2.7 cP [4]) was indeed higher than that of the KH solution (0.76 cP), but was clearly lower than that of blood at physiological shear rates (4-6 cP [14,15]). Embolism by PFC particles could not be the reason for the low flow rates: the flow rates reached their original high values during the following KH perfusion, obstruction of capil laries could not be observed histologically.…”

“…Oberursel, FRG). The measurement of other physicochemical parameters has been described elsewhere (4). The emulsion has already been proved to be reliable in longtime perfu sions of the brain [5] and spinal cord of rats [4], Male and female guinea pigs of the MariothFalben strain, weighing 350-400 g. were killed by a blow on the neck.…”

“…The measurement of other physicochemical parameters has been described elsewhere (4). The emulsion has already been proved to be reliable in longtime perfu sions of the brain [5] and spinal cord of rats [4], Male and female guinea pigs of the MariothFalben strain, weighing 350-400 g. were killed by a blow on the neck. The hearts were removed and per fused with a KH solution for about 30 min (adaptation period) according to the method of Langendorff [ 10], Then, in a first series of experiments, they were per fused during an initial period of 10 min with the KH solution, in the second period of about 30 min with the PFC emulsion, and in the third period of 20 min again with the KH solution, in another series of exper iments.…”

“…7], and spinal cord [4] better and longer than perfu sion without a special oxygen carrier in the medium. The purpose of this study was to investigate whether the ultrastructurc and function of the isolated perfused guinea pig heart could be maintained during perfusion with a PFC emulsion.…”