“Perfusion chromatography”. The effects of intra-particle convective velocity and microsphere size on column performance

Protein adsorption‐dependent electro‐kinetic pore flow: Modeling of ion‐exchange electrochromatography with an oscillatory transverse electric field

“…Tallarek et al [12,13] quantitatively studied the electro-kinetic transport in single porous media (no solute binding) by confocal laser scanning microscopy. They showed pictures of intraparticle excursion profiles of protein in the presence of EF, and obtained consistent modeling results for neutral analyte intraparticle distribution with those with perfusive flow driven by pressure [14]. However, the short-time (0.5 s) simulation obviated the evolution of mass transfer.…”

“…This indicates the enhanced convective mass transfer rate with increasing the field strength, which led to increased DBC in the pIEEC. Unlike the convection in macro-pores for perfusion chromatography [14], it was found that a constant Peclet number could not give a satisfying simulation of the breakthrough curves of BSA in the pIEEC. This inspired us to regard the intra-particle velocity and/or the effective diffusion coefficient in Eq.…”

The Interplay of Diffusional and Electrophoretic Transport Mechanisms of Charged Solutes in the Liquid Film Surrounding Charged Nonporous Adsorbent Particles Employed in Finite Bath Adsorption Systems

“…[12], δ o represents the value of the charge density, δ, on the surface of the particles at t = 0. Equation [13] is obtained from the consideration that the electric field…”

“…Such small in diameter nonporous adsorbent particles provide a large surface area for adsorption, fast uptake rates, and high resolution because they do not involve the slow intraparticle mass transfer mechanisms that characterize the dynamic behavior of the adsorption of proteins in porous adsorbent particles . The small in diameter nonporous adsorbent particles can be employed in a finite bath (batch) adsorption (7,8,11) system, as well as in a chromatographic column (packed bed) operated in the fixed-bed or periodic counter-current mode (1-10, 12-15, 17-26).…”

Development and Application of Liquid Chromatography in Life Sciences

“…Other methods to overcome pore-diffusion effects trigger the introduction of hydrophobic interaction chromatography (HIC) and ion-exchange and affinity chromatography [17] due to retention, which occurs not inside of microparticles but at the outer surface. Actually, the development of robust adsorbents for process-scale chromatography has been increased fast from the late 1960s.…”