Performing Vitreous Biopsy by Perfluorocarbon-Perfused Vitrectomy

Quiroz‐Mercado, Hugo; Rivera–Sempertegui, J.O.; Macky, Tamer A.; Navarro-López, P; Griselda-Alvarez, Lidia; Ibarra–Ponce, N.; Moreno-Páramo, Daniel

doi:10.1016/j.ajo.2005.07.028

Cited by 27 publications

(13 citation statements)

References 6 publications

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“…Thus, filling the eye from posterior to anterior (Kramer et al 1995) and making it an ideal tool to gain undiluted vitreal material without risking a sudden decrease in intraocular pressure. Even if perfluorocarbon liquids get mixed with the vitreal specimen, postoperative separation by different gravities or by freezing the specimen can be carried out (Quiroz-Mercado et al 2005). Since there was no information about any utilisation of perfluorocarbon liquids or air instead of flushing solution in equine ocular surgery and adverse effects in equine patients are unknown, this tool was not used on the equine patients undergoing vitrectomy in this study.…”

Section: Discussionmentioning

confidence: 99%

“…Some authors described obtaining undiluted vitreal material during vitrectomy of horses with ERU by aspiration of the vitreal material at the beginning of the pars plana vitrectomy, before opening the infusion line with balanced salt solution (Loibl 2009, Popp 2011, Wiehen 2012. Complications, such as retinal bleeding, pupil constriction and choroidal haemorrhages, were described in human patients for sampling the vitreous without immediate volume replacement (Quiroz-Mercado et al 2005). Complications recorded during pars plana vitrectomy in equine patients are retinal detachment and vitreal and/or retinal haemorrhage (Frühauf 1998, von Borstel et al 2005, Werry and Gerhards 1991, Werry et al 1992.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection of intraocular leptospiral DNA, antibodies and Leptospira spp. in horses with equine recurrent uveitis in different laboratories

Baake¹,

Borstel²,

Rohn³

et al. 2016

PHK

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Summary: Equine recurrent uveitis (ERU) is an ocular disease characterized mainly by recurrent episodes of inflammation, alternating with quiescent episodes of various durations. There are numerous aetiological theories for the cause of ERU, but to date, the "classic ERU" is most commonly associated with an intraocular leptospiral infection. The pars plana vitrectomy has, so far, been the most promising treatment of ERU and is thought to be more successful in eyes with detectable leptospiral antibodies in the microscopic agglutination test (MAT) and therefore a preselection is recommended. The objective of this study was to investigate whether different laboratories have an influence on the frequency of detection of leptospiral DNA and leptospiral antibodies from vitreal samples of horses with ERU and if these results are in accordance with cultural testing of the vitreal samples. Vitreal samples were obtained from 93 eyes of 78 horses of various breeds and age with a clinical history of ERU. The vitreal samples were taken at the beginning of the vitrectomy to keep the dilution of the vitreal samples as low as possible. The samples were split and sent to three different laboratories in Germany (L1, L2 and L3). Laboratory 1 (L1) performed a polymerase chain reaction (PCR) and a MAT, laboratory 2 (L2) carried out a PCR and laboratory 3 (L3) performed a MAT, a cultural examination and an enzyme-linked immunosorbent assay on the vitreal specimens. The amount of dilution was evaluated by comparing the urea content of 34 vitreal samples with the urea content of the serum of each of the horses at the time of sampling. Furthermore, a serial dilution was performed with undiluted vitreal material of two enucleated eyes and sent to L1 for MAT and PCR. The results of the PCR indicated a strong conformity between L1 and L2 (Kappa 0.95), both laboratories concurrently revealed 52 positive and 31 negative results and only two specimens showed deviating results. The MAT in L1 was positive in 51% of the vitreal samples tested, whereas only 41% of the vitreal samples were tested positive in L3. The conformity was, moreover, serovar-and breed-dependent. The Kappa was 0.67 when only including serovar Grippotyphosa, and was 0.82 for serovar Pomona (Tab. 5). Warmblood horses displayed a Kappa of 0.56 between L1 and L3, whereas the Kappa for the Icelandic horses was 0.91 between L1 and L3. The cultivation of leptospires was successful in 16 % of the vitreal specimens. The dilution of vitreal samples was measured to range between 1 and 2.1 with a standard deviation of 1.68. Overall, 67 vitreal samples were tested simultaneously in all three laboratories. A positive result in at least one of the tests performed occurred in 57 % (38), whereas 43 % (29) of the vitreal specimens were tested negative in all five tests performed (PCR in L1 and L2, MAT in L1 and L3, and culture in L3). The variation of agreement of the MAT between serovar Grippotyphosa and serovar Pomona and between Warmblood and Icelandic horses in L1 and L3 might be account...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Detection of intraocular leptospiral DNA, antibodies and Leptospira spp. in horses with equine recurrent uveitis in different laboratories

Baake¹,

Borstel²,

Rohn³

et al. 2016

PHK

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“…The infusion is then started, and diluted vitreous is aspirated into a second syringe. The amount of vitreous obtained is limited by complication of hypotony such as suprachoroidal hemorrhage; therefore, QuirozMercado et al [2] described perfluorocarbon-perfused vitrectomy, in which balanced salt solution is replaced with perfluorocarbon liquid during vitreous aspiration. Using this method, the authors were able to obtain an average of 2.24 ml of undiluted vitreous.…”

Section: Obtaining Handling and Processing Vitreous Specimensmentioning

confidence: 98%

Diagnostic vitrectomy for the diagnosis and management of posterior uveitis of unknown etiology

Margolis

2008

Current Opinion in Ophthalmology

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“…Approximately 1.5–2 mL of vitreous fluid can be obtained in this fashion. To prevent complications of hypotony, some authors have suggested air, perfluoron infusion or digital pressure on the eye wall to maintain intraocular pressure [44–46]. However, the potential for lens feathering following air infusion and the need to remove the air in both phakic and pseudophakic patients may make air less desirable.…”

Section: Surgical Techniquementioning

confidence: 99%

Diagnostic Vitrectomy for Primary Intraocular Lymphoma

Hwang¹,

Yeh²,

Bergstrom³

2014

International Ophthalmology Clinics

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Primary intraocular lymphoma (PIOL) can present with uveitis, vitritis, or chorioretinal lesions. While fluorescein angiography, fundus autofluorescence, and optical coherence tomography may aid in the diagnosis of PIOL, a definitive diagnosis can only be achieved with tissue analysis. The specimen may be obtained with a vitreous or aqueous tap, however, a diagnostic pars plana vitrectomy with acquisition of diluted and undilated vitreous fluid or a chorioretinal biopsy is preferable. Once a specimen is obtained, cytopathology, flow cytometry, immunohistochemistry, gene rearrangement studies with polymerase chain reaction, and cytokine analysis can be performed to confirm the diagnosis of PIOL. Treatment is dependent on the presentation of PIOL and may include systemic chemotherapy, local intravitreal chemotherapy, radiation or a combination of therapies.

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Performing Vitreous Biopsy by Perfluorocarbon-Perfused Vitrectomy

Cited by 27 publications

References 6 publications

Detection of intraocular leptospiral DNA, antibodies and Leptospira spp. in horses with equine recurrent uveitis in different laboratories

Detection of intraocular leptospiral DNA, antibodies and Leptospira spp. in horses with equine recurrent uveitis in different laboratories

Diagnostic vitrectomy for the diagnosis and management of posterior uveitis of unknown etiology

Diagnostic Vitrectomy for Primary Intraocular Lymphoma

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