Performances of different diagnostic tests for feline infectious peritonitis in challenging clinical cases

Abstract

IHC must always be performed to confirm FIP. If this is not possible, when histopathology is controversial, elevated AGP concentrations may support the diagnosis of FIP.

Application of native agarose gel electrophoresis of serum proteins in veterinary diagnostics

“…Immunoglobulins usually migrate in the γ-globulin region, and some (together with proteins of the complement system) migrate in the β-globulin region. The increase in production of these proteins varies in magnitude but in certain cases it can be considerable (17). Gammopathies underlying chronic inflammation are usually polyclonal (as in the case of canine ehrlichiosis and feline infectious peritonitis).…”

“…Serum is the body fluid most frequently used for electrophoretic analysis, but body cavity fluids, urine, and cerebrospinal fluid are also used in diagnostics (14,17,49). In the case of the last two, however, the sample must first be concentrated, as the amount of protein they contain is often insufficient to ensure that fractions will be detected with the necessary sensitivity after separation.…”

Feline Coronavirus in Multicat Environments

“…Acute-phase proteins are a class of proteins whose plasma concentrations increase or decrease in response to inflammatory disorders. ␣ 1 -acid glycoprotein levels greater than 1.5 g/L in plasma or effusions are suggestive of FIP, 144 with diagnostic Abbreviations: A:G ratio, albumin to globulin ratio; ELISA, enzyme-linked immunosorbent assay; IFA, immunofluorescent antibody assay; mRNA, messenger RNA; NPV, negative predictive value; PPV, positive predictive value; RT-PCR, reverse transcriptase polymerase chain reaction. a Calculated based on concatenated data from original studies.…”

Acute Phase Proteins as Markers of Infectious Diseases in small Animals / Proteini Akutne Faze Kao Markeri Infektivnih Bolesti Malih Životinja

“…Serum AGP concentration higher than 1.5 g/L is the cut-off value in differentiating naturally occurring infection from cats with clinical signs similar to FIP but not suffering from this disease [14]. Additionally, serum AGP values ranged between 2.04 to 14 mg/mL in cats with FIP confi rmed by immunohistochemistry, with a diagnostic concordance of 100% specifi city and sensitivity [53].…”

“…However, the studies are currently restricted to a limited number of investigators across the world as the majority of veterinary laboratories do not have APPs assay kits available and to date there are no suffi cient published results concerning the specifi cities, sensitivities and applications of these tests [58]. [14,52,53,56] …”