Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection

Lee, Hwa-Eun; Jeon, Yu-Bin; Chin, Bryan A.; Lee, Sang Hyuk; Lee, Hye Jin; Park, Mi-Kyung

doi:10.1016/j.foodchem.2022.135239

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…As described previously, the Fe 3 O 4 particles were grafted with poly(dopamine) [ 15 , 47 ]. Briefly, 5.0 g of magnetic nanoparticle was transferred into Tris-HCl buffer solution (10 mmol/L, pH 8.5, 250 mL) containing ethyl alcohol (75 mL) and 800 mg dopamineˑ HCl.…”

Section: Methodsmentioning

confidence: 99%

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Beyazit,

Arica,

Acikgoz-Erkaya

et al. 2024

Microchim Acta

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A fast and accurate identification of Listeria monocytogenes. A new quartz crystal microbalance (QCM) aptasensor was designed for the specific and rapid detection of L. monocytogenes. Before detection of the target bacterium from samples in the QCM aptasensor, a magnetic pre-enrichment system was used to eliminate any contaminant in the samples. The prepared magnetic system was characterized using ATR-FTIR, SEM, VSM, BET, and analytical methods. The saturation magnetization values of the Fe3O4, Fe3O4@PDA, and Fe3O4@PDA@DAPEG particles were 57.2, 40.8, and 36.4 emu/g, respectively. The same aptamer was also immobilized on the QCM crystal integrated into QCM flow cell and utilized to quantitatively detect L. monocytogenes cells from the samples. It was found that a specific aptamer-magnetic pre-concentration system efficiently captured L. monocytogenes cells in a short time (approximately 10 min). The Fe3O4@PDA@DA-PEG-Apt particles provided selective isolation of L. monocytogenes from the bacteria-spiked media up to 91.8%. The immobilized aptamer content of the magnetic particles was 5834 µg/g using 500 ng Apt/mL. The QCM aptasensor showed a very high range of analytical performance to the target bacterium from 1.0 × 102 and 1.0 × 107 CFU/mL. The limit of detection (LOD) and limit of quantitation (LOQ) were 148 and 448 CFU/mL, respectively, from the feeding of the QCM aptasensor flow cell with the eluent of the magnetic pre-concentration system. The reproducibility of the aptasensor was more than 95%. The aptasensor was very specific to L. monocytogenes compared to the other Listeria species (i.e., L. ivanovii, L. innocua, and L. seeligeri) or other tested bacteria such as Staphylococcus aureus, Escherichia coli, and Bacillus subtilis. The QCM aptasensor was regenerated with NaOH solution, and the system was reused many times. Graphical Abstract

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Section: Methodsmentioning

confidence: 99%

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Beyazit,

Arica,

Acikgoz-Erkaya

et al. 2024

Microchim Acta

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“…However, the sensitivity of MCE is limited because the sampling volume is as low as nanoliter or picoliter. During these years, phages probes based sensors have received much attention due to their high speci city, sensitivity, and simplicity [30][31][32][33]. Phages are virus which can speci cally recoginize the bacteria strain and eliminate them Phage based sensors exhibits obvious advantages in distinguishing live bacteria from dead bacteria which make up for this de ciency of antibodies and aptamers [34,35].…”

Section: Introductionmentioning

confidence: 99%

Magnetic poly(phages) encoded probes based dual-mode assay for rapid determination of live Escherichia coli and Hafnia paralvei based on microfluidic chip and ATP bioluminence meter

Wang,

You

et al. 2024

Preprint

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The detection of multiplex live pathogens in spoilage aquatic products is critically important. In this study, a dual-mode assay for screening and detecting live Escherichia coli (E. coli) and Hafnia paralvei (H. paralvei) (as two typical pathogens in aquatic products) based on magnetic poly (phages) encoded probes (MPEP) was developed. The probes were prepared by grafting a large number of phages targeted different target bacteria on a long-chain DNA structure, respectively. They could specifically capture and enrich E. coli and H. paralvei by magnetic separation. Then, different DNA signal tags with different lengths would conjugate with the corresponding MPEP-bacteria complex and form two kinds of sandwich structures, respectively. After that, the captured E. coli and H. paralvei were lysed to release both adenosine triphosphate (ATP) and DNA signal tags. The measurement includes two steps. Firstly, a portable ATP bioluminescence meter was employed to rapidly screen the positive samples that contain either of the two target bacteria. Secondly, only positive samples were injected into the microfluidic chip which could detect various DNA signal tags for accurate quantification of the target bacteria. The assay demonstrated high sensitivity (3 CFU/mL for E.coli and 5 CFU/mL for H. paralvei), high specificity (strain identification), signal amplification (20 folds) and short time(≤ 35min). It can be applied to detect other pathogens only by changing the relative phage in MPEP. Furthermore, the proposed dual-mode assay provides a wide prospect for rapid screening and accurate determination of live foodborne pathogens.

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Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity

Choe,

Kim,

Han

et al. 2023

J Microbiol.

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Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection

Cited by 6 publications

References 29 publications

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Quartz crystal microbalance–based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples

Magnetic poly(phages) encoded probes based dual-mode assay for rapid determination of live Escherichia coli and Hafnia paralvei based on microfluidic chip and ATP bioluminence meter

Prevalence of Indigenous Antibiotic-Resistant Salmonella Isolates and Their Application to Explore a Lytic Phage vB_SalS_KFSSM with an Intra-Broad Specificity

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