Magnetic poly(phages) encoded probes based dual-mode assay for rapid determination of live Escherichia coli and Hafnia paralvei based on microfluidic chip and ATP bioluminence meter
Ye Wang,
Ming Wang,
Hang You
et al.
Abstract:The detection of multiplex live pathogens in spoilage aquatic products is critically important. In this study, a dual-mode assay for screening and detecting live Escherichia coli (E. coli) and Hafnia paralvei (H. paralvei) (as two typical pathogens in aquatic products) based on magnetic poly (phages) encoded probes (MPEP) was developed. The probes were prepared by grafting a large number of phages targeted different target bacteria on a long-chain DNA structure, respectively. They could specifically capture an… Show more
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