Performance of Routine<i>Helicobacter pylori</i>Invasive Tests in Patients with Dyspepsia

Lee, Hsi‐Chang; Huang, Ting-Chang; Lin, Chin-Lin; Chen, Kuan-Yang; Wang, Chung-Kwe; Wu, Deng‐Chyang

doi:10.1155/2013/184806

Cited by 16 publications

(12 citation statements)

References 20 publications

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“…Siddique et al (2008) demonstrated a much higher sensitivity of the rapid urease test, when increased numbers of antral samples were taken from one (52%) to four samples (96%). This also concurred with the work by Lee et al (2013), which showed an increased sensitivity when two biopsy specimens were taken from the antrum and corpus regions (85.6%), in comparison to only a single antrum biopsy (64.6%), and a single corpus biopsy (69.5%).…”

Section: Clarithromycin Resistancesupporting

confidence: 90%

Helicobacter pylori Infection: Prevalence, Demographic Characteristics, Clarithromycin Resistance and Evaluation of the In-House Rapid Urease Test in Sungai Buloh Hospital, Malaysia

Mokhtar¹,

Muttaqillah²,

Hussin³

et al. 2019

JSM

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Helicobacter pylori infection remains an essential global research focus, and increasing clarithromycin resistance was reported to impact the efficacy of clarithromycin-based treatment regiments. The study objectives sought to understand the prevalence of H. pylori infection amongst patients from Sungai Buloh Hospital, Malaysia, its association with demographic factors, and the clarithromycin resistance rate. The in-house rapid urease test (IRUT) was also evaluated and compared to the Campylobacter-like organism (CLO) test using culture or histopathological testing as the gold standard for diagnosis. The gastric corpus biopsies of 352 patients were included, as well as their age group, gender and ethnicity. Clarithromycin susceptibility was measured using the E-test method. The overall prevalence of H. pylori infection was 15.1% (53/352). There was no significant association between the age groups, gender and ethnicity with regards to H. pylori infection. Four of the 13 viable isolates (30.8%) were clarithromycin-resistant. Although IRUT had a slightly lower specificity (94.9%) than that of the CLO test (95.9%), both tests had the same sensitivity values (81.1%). IRUT had a lower positive predictive value (74.1%) than the CLO test (78.2%) but showed a similar negative predictive value (96.5%) compared to the CLO test (96.6%). Both tests displayed a very good agreement (κ = 0.97). In conclusion, the overall prevalence of H. pylori infection in our study was generally low. The high proportion of clarithromycinresistant isolates may not reflect the exact resistance rate due to the small number of positive cultures. Our IRUT is an acceptable alternative to the CLO test for the rapid diagnosis of H. pylori infections based on its comparable performance.

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Section: Clarithromycin Resistancesupporting

confidence: 90%

Helicobacter pylori Infection: Prevalence, Demographic Characteristics, Clarithromycin Resistance and Evaluation of the In-House Rapid Urease Test in Sungai Buloh Hospital, Malaysia

Mokhtar¹,

Muttaqillah²,

Hussin³

et al. 2019

JSM

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“…This finding suggests that we can obtain a sufficient amount of DNA (more than 50 ng/mL) for DNA sequencing analysis from one piece of a gastric antral biopsy specimen. Considering that the sensitivity of diagnostic H pylori cultures by experienced laboratories has been reported as 50–92%, [ 20 – 22 ] 23S rRNA DNA mutation analysis has excellent sensitivity compared to H pylori cultures. Second, DNA sequencing of domain V in the 23S rRNA gene revealed only 2 major point mutations, 2143A>G and 2182C>T. Other point mutations, such as 2142A>G, 2190T>C and 2195C>T, showed low mutation rates (below 2%), and mutations at nucleotide positions 2115, 2144, 2711, 2223, and 2244, which were reported to confer clarithromycine resistance, were not found in our participants.…”

Section: Discussionmentioning

confidence: 99%

Clinical relevance of point mutations in the 23S rRNA gene in Helicobacter pylori eradication

Park

Kim²,

Lee

et al. 2018

Medicine

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Clarithromycin-based triple therapy is prescribed worldwide for Helicobacter pylori eradication. However, increases in the clarithromycin resistance of H pylori are thought to be responsible for eradication failure. Here, we studied whether point mutations in domain V of the 23S rRNA gene can affect H pylori eradication failure in a prospective, open-label, observational study. Of the 755 enrolled patients, 299 patients (39.6%) had positive Campylobacter-like organism (CLO) tests. DNA sequencing analysis of H pylori 23S rRNA in 295 patients revealed that 2143G was the most frequent point mutation (24.7% of patients), followed by the 2182T mutation (11.5%). The overall eradication failure rate was 20.9% (42/201) in clarithromycin-based triple therapy. Patients with the 2143G had an approximately 60% eradication failure rate, which suggested that 2143G was a high-risk genotype for eradication failure. Patients with the 2182C genotype without 2143G had an 8.7% failure rate, and patients without 2143G or 2182C had only a 4.3% failure rate. The presence of 2143G, which was associated with previous eradication history and female sex, was an independent risk factor for eradication failure. In conclusion, the 2143G point mutation in the 23S rRNA of H pylori was an independent risk factor for eradication failure in clarithromycin-based triple therapy. Personalized tailored therapy based on the genotypes of 23S rRNA can increase eradication success rates in H pylori infections.

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“…Non-invasive diagnostic tests for detection the antigen and antibody directed against H. pylori are now available and these tests are valuable both for screening and evaluation of therapy (Lee et al, 2013), and these noninvasive tests are also easy and cheap to perform (Formichella et al, 2013). The advantage of antigen detection test is to evaluate the eradication of H. pylori infection.…”

Section: Introductionmentioning

confidence: 99%

Screening for the prevalence of Helicobacter pylori infection among dyspeptic patients using simple fecal antigen and serum antibody diagnostic methods at Mukalla city Hospitals, Hadhramout, Yemen

Hameed¹,

Barajash²

2021

Afr. J. Microbiol. Res.

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Helicobacter pylori is the most common infection in the world, and the most main causes of dyspepsia are related to H. pylori infection. In that respect, several non-invasive methods for the diagnosis of H. pylori infection were utilized. The current study aimed to determine the prevalence of H. pylori infection and its associated variables among dyspeptic patients in Mukalla city, Hadhramout-Yemen during a period from February to September 2018. A cross-sectional descriptive study was conducted among 100 suspected dyspeptic patients. Data regarding to associated variables were collected using a structured questionnaire, and the samples of feces and serum were collected and screened for H. pylori by rapid diagnostic immunochromatographic assays for antigen and antibody. Data was analyzed using SPSS statistical software version 20 and a P value <0.05 was taken statistically significant. Prevalence of H. pylori was found to be 15 and 18.5% using the antigen H. pylori test and serum anti-H. pylori test respectively. Prevalence using antigen H. pylori test was significantly associated to male sex (P=0.035). In those patients withH. pylori, a positive result with antigen test was significantly associated to drinking non-filtered water (COR = 3.67; 95%CI=1.436-9.363; P=0.007), symptoms of heartburn and regurgitation (COR=0.865, 95%CI=0.034-0.536, P=0.004) and antibiotics used (COR=0.312, 95%CI=0.125-0.780, P=0.013). The prevalence of H. pylori infection was frequent among the dyspeptic patients in the study area. H. pylori infection was related to non-filtered water source, symptoms of heartburn and regurgitation and antibiotics used are contributing factors. Moreover, further studies are needed to investigate other potential associated variables for H. pylori infection.

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Performance of RoutineHelicobacter pyloriInvasive Tests in Patients with Dyspepsia

Cited by 16 publications

References 20 publications

Helicobacter pylori Infection: Prevalence, Demographic Characteristics, Clarithromycin Resistance and Evaluation of the In-House Rapid Urease Test in Sungai Buloh Hospital, Malaysia

Helicobacter pylori Infection: Prevalence, Demographic Characteristics, Clarithromycin Resistance and Evaluation of the In-House Rapid Urease Test in Sungai Buloh Hospital, Malaysia

Clinical relevance of point mutations in the 23S rRNA gene in Helicobacter pylori eradication

Screening for the prevalence of Helicobacter pylori infection among dyspeptic patients using simple fecal antigen and serum antibody diagnostic methods at Mukalla city Hospitals, Hadhramout, Yemen

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