Performance of rapid influenza testing in hospitalized children

Stripeli, Fotini; Sakkou, Zoi; Papadopoulos, Nikolaos G.; Georgiou, Vassiliki; Gratsia, P.; Christodoulou, Ioannis; Τσολιά, Μαρία

doi:10.1007/s10096-010-0914-2

Cited by 14 publications

(10 citation statements)

References 30 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several rapid test kits that can provide results within 15 min are available. The performance of these tests varies [26,27], but is usually good in children and during the early course of the illness when, also, the benefits of the antiviral treatment of influenza are greatest [10,25,28,29]. Pre-test probability of influenza Fig.…”

Section: Discussionmentioning

confidence: 98%

Signs and symptoms predicting influenza in children: a matched case–control analysis of prospectively collected clinical data

Heinonen

Peltola

Silvennoinen

et al. 2011

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

We aimed to determine whether there are signs or symptoms that could help clinicians to distinguish between influenza and other respiratory infections. The clinical data for this matched case-control analysis were derived from a 2-year prospective cohort study of respiratory infections among children aged≤13 years. At any signs of respiratory infection, the children were examined and nasal swabs were obtained for virologic analyses. Cases were 353 children with laboratory-confirmed influenza and controls were 353 children with respiratory symptoms who tested negative for influenza. Cases and controls were matched for gender, age, and timing of the visit. In the multivariate conditional logistic regression analyses, fever was the only sign that independently predicted influenza virus infection, with odds ratios ranging from 13.55 (95% confidence interval [CI], 6.90-26.63) to 50.10 (95% CI, 16.25-154.45), depending on the degree of fever. In all analyses, the predictive capability of fever increased with incremental elevations in the child's temperature. The likelihood ratio of fever≥40.0°C in predicting influenza was 6.00 (95% CI, 2.80-12.96). Among unselected children seen as outpatients during influenza outbreaks, fever is the only reliable predictor of influenza virus infection. The optimal use of influenza-specific antiviral drugs in children may require virologic confirmation.

show abstract

Section: Discussionmentioning

confidence: 98%

Signs and symptoms predicting influenza in children: a matched case–control analysis of prospectively collected clinical data

Heinonen

Peltola

Silvennoinen

et al. 2011

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

show abstract

“…Fifty‐nine children with fever and/or respiratory symptoms confirmed by physician diagnosis were enrolled in the study after informed consent was given. Viral RNA was extracted from nasopharyngeal aspirates, and RV detection was performed using RT‐PCR (18, 19). Rabbit antibodies against VP1 were obtained by immunizing rabbits with recombinant VP1 (20), whereas RV89‐specific guinea pig antiserum was obtained from the American Type Culture Collection (ATCC; Manassas, VA. USA).…”

Section: Methodsmentioning

confidence: 99%

Misdirected antibody responses against an N‐terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections

et al. 2011

View full text Add to dashboard Cite

Rhinoviruses (RVs) are the primary cause of upper respiratory tract infections, generally known as the common cold. Moreover, RV infections can trigger severe exacerbations of asthma and chronic obstructive pulmonary disease (COPD). We expressed the 4 major RV capsid proteins, VP1-VP4, in Escherichia coli and used these proteins as well as recombinant and synthetic VP1 fragments to study and map antibody responses in RV-infected humans. VP1, which on infection binds to ICAM 1, was identified as a major target for the memory immune response, residing in the IgG1 subclass and IgA class. Interestingly, this response was mainly directed against an N-terminal 20 mer peptide in VP1, P1a, which becomes exposed on intact RV only when it docks to its receptor ICAM 1. Molecular modeling using the 3-dimensional RV capsid structures revealed that P1a was localized inside the capsid and outside the areas involved in receptor binding or RV neutralization. Our results suggest misdirection of antibody responses against a nonprotective epitope as a mechanism how RV escapes immunity and causes recurrent infections. Based on these findings, it may be possible to design vaccines against RV infections and RV-induced respiratory diseases.

show abstract

“…Nilsson et al 83 found the sensitivity of the BinaxNOW influenza A/B test against PCR to drop from 71% to 63% and 14% when specimens were collected from adults presenting to the emergency department of Malmö University Hospital on days 1-3, 1-5 and > 5 days, respectively, following onset of symptoms. Similarly, Stripeli et al 84 observed a fall in sensitivity from 76% to 65% for The QuickVue…”

Section: Discussionmentioning

confidence: 79%

Randomised controlled trial and health economic evaluation of the impact of diagnostic testing for influenza, respiratory syncytial virus and Streptococcus pneumoniae infection on the management of acute admissions in the elderly and high-risk 18- to 64-year-olds

Nicholson

Abrams

Batham

et al. 2014

Health Technology Assessment

View full text Add to dashboard Cite

Performance of rapid influenza testing in hospitalized children

Cited by 14 publications

References 30 publications

Signs and symptoms predicting influenza in children: a matched case–control analysis of prospectively collected clinical data

Signs and symptoms predicting influenza in children: a matched case–control analysis of prospectively collected clinical data

Misdirected antibody responses against an N‐terminal epitope on human rhinovirus VP1 as explanation for recurrent RV infections

Randomised controlled trial and health economic evaluation of the impact of diagnostic testing for influenza, respiratory syncytial virus and Streptococcus pneumoniae infection on the management of acute admissions in the elderly and high-risk 18- to 64-year-olds

Contact Info

Product

Resources

About