Performance of pulsed plate bioreactor for biodegradation of phenol

Shetty, K. Vidya; Kalifathulla, I.; Srinikethan, G.

doi:10.1016/j.jhazmat.2006.09.058

Cited by 37 publications

(38 citation statements)

References 53 publications

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“…the biodegradation of phenol by an unadapted mixed microbial culture isolated from soil, has been studied in a packed-bed reactor using peat media (24). the interest in the immobilization of microorganisms has grown in recent years (31,36,37). immobilized cells have an advantage over native cells due to the possibility for multiple applications for an extended period of time.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells ofAspergillus AwamoriandTrichosporon Cutaneum

Yordanova

Godjevargova

Nenkova

et al. 2013

Biotechnology & Biotechnological Equipment

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Section: Introductionmentioning

confidence: 99%

Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells ofAspergillus AwamoriandTrichosporon Cutaneum

Yordanova

Godjevargova

Nenkova

et al. 2013

Biotechnology & Biotechnological Equipment

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“…To enhance the biodegradation capabilities of micro organisms different approaches were applied (Shetty et al, 2007;Li et al, 2013). For example, immobiliza tion technique could lead to the accomplishment of quite effective technological processes for bioreme diation of contaminated sites (Yordanova et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Hristov

Christova

Kabaivanova

et al. 2016

Polish Journal of Microbiology

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A b s t r a c tThe capability of the biosurfactantproducing strain Rhodococcus wratislawiensis BN38 to mineralize both aromatic and aliphatic xeno biotics was proved. During semicontinuous cultivation 11 g/l phenol was completely degraded within 22 cycles by Rhodococcus free cells. Immobilization in a cryogel matrix was performed for the first time to enhance the biodegradation at multiple use. A stable simultaneous hydrocarbon biodegradation was achieved until the total depletion of 20 g/l phenol and 20 g/l nhexadecane (40 cycles). The alkanotrophic strain R. wratislawiensis BN38 preferably degraded hexadecane rather than phenol. SEM revealed well preserved cells entrapped in the heterogeneous supermacroporous structure of the cryogel which allowed unhindered mass transfer of xenobiotics. The immobilized strain can be used in real conditions for the treatment of contaminated industrial waste water. 288of materials due to their unique heterogeneous open porous structure, which significantly increases the equilibrium sorption properties and allows unhindered diffusion of solutes, nanoparticles and microparticles. Usually, cryogels possess spongylike structure of huge pores (50-200 µm) containing free water surrounded by thin walls and, therefore, they are often used for immo bilization of enzymes and cells by entrapment inside the channels of interconnected pores (Lozinsky et al., 2003).Many bacterial strains have been isolated with the abilities to degrade nhexadecane and phenol sepa rately, which are often used to represent the aliphatic and aromatic pollutant (Yordanova et al., 2009; Abdel Megeed et al., 2010;Tambekar et al., 2012). However, few strains have been reported to have the dual abilities (Sun et al., 2012).This paper aims to report the first study on simul taneous biodegradation of nhexadecane and phenol by Rhodococcus wratislawiensis BN38 immobilized in hydroxypropylcellulose/poly (Nisopropylacrylamide) cryogel matrix. Experimental Materials and MethodsMicroorganism, media and cultivation. The R. wrati slawiensis BN38, employed in this study was isolated from soil polluted with hydrocarbons by a standard enrichment technique (Tuleva et al., 2008 O, 0.2 and phenol and nhexadecane at 500 mg/l, unless otherwise mentioned, was used. The pH of the medium was adjusted to 7.0. The MSM was solidified as MSM phenol agar by addition of 1.8% agar when necessary. Cultures grown on MSM agar supplemented with 500 mg/l phenol were used to inoculate 500 ml Erlenmeyer flasks, containing 100 ml liquid MSM. At each cycle MSM was supplemented to 100 ml with fresh sterile medium without cell transfer and only phenol was added. Cultures were incubated during the longterm semicontinuous biodegradation processes while shaking (120 rpm) at 29°C. Inocula with cell density (OD 610 nm) of 0.4 were employed in the processes of biodegradation. The pH values were 6.7-6.8 all the time, due to the buffering activity of the nutrient medium used.Bacterial growth was assessed by determination of the optical density (OD 610 nm) of t...

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“…With viable cells, growth and gas evolution can lead to significant mechanical disruption of the immobilising matrix [9]. A recent innovation in immobilised cell or fixed film bioreactors is the pulsed plate bioreactor (PPBR) with immobilised cells [10]. The advantages of this kind of bioreactor over other immobilised cell bioreactors are presented elsewhere [10].…”

Section: Introductionmentioning

confidence: 99%

“…A recent innovation in immobilised cell or fixed film bioreactors is the pulsed plate bioreactor (PPBR) with immobilised cells [10]. The advantages of this kind of bioreactor over other immobilised cell bioreactors are presented elsewhere [10]. Shetty et al [10] have studied the performance of a pulsed plate bioreactor with immobilised cells for aerobic biodegradation of phenol and found its potentiality to be an efficient bioreactor for wastewater treatment.…”

Section: Introductionmentioning

confidence: 99%

“…The novelty of the work lies in the use of a theoretical model to predict steady-state biodegradation in a pulsed plate bioreactor with immobilised cells of NCh. This microorganism is an actinomycete and was chosen for the degradation of phenol, as it was found to degrade phenol effectively and, being found in soil, it was more resistant to contamination [10,14].…”

Section: Introductionmentioning

confidence: 99%

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Modelling and simulation of steady-state phenol degradation in a pulsed plate bioreactor with immobilised cells of Nocardia hydrocarbonoxydans

Shetty

Verma

Srinikethan

2010

Bioprocess Biosyst Eng

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A novel bioreactor called pulsed plate bioreactor (PPBR) with cell immobilised glass particles in the interplate spaces was used for continuous aerobic biodegradation of phenol present in wastewater. A mathematical model consisting of mass balance equations and accounting for simultaneous external film mass transfer, internal diffusion and reaction is presented to describe the steady-state degradation of phenol by Nocardia hydrocarbonoxydans (Nch.) in this bioreactor. The growth of Nch. on phenol was found to follow Haldane substrate inhibition model. The biokinetic parameters at a temperature of 30 ± 1 °C and pH at 7.0 ± 0.1 are μ (m) = 0.5397 h(-1), K (S) = 6.445 mg/L and K (I) = 855.7 mg/L. The mathematical model was able to predict the reactor performance, with a maximum error of 2% between the predicted and experimental percentage degradations of phenol. The biofilm internal diffusion rate was found to be the slowest step in biodegradation of phenol in a PPBR.

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Performance of pulsed plate bioreactor for biodegradation of phenol

Cited by 37 publications

References 53 publications

Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells ofAspergillus AwamoriandTrichosporon Cutaneum

Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells ofAspergillus AwamoriandTrichosporon Cutaneum

Simultaneous Biodegradation of Phenol and n-Hexadecane by Cryogel Immobilized Biosurfactant Producing Strain Rhodococcus wratislawiensis BN38

Modelling and simulation of steady-state phenol degradation in a pulsed plate bioreactor with immobilised cells of Nocardia hydrocarbonoxydans

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