Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells of<i>Aspergillus Awamori</i>and<i>Trichosporon Cutaneum</i>

Yordanova, Galina; Godjevargova, Tzonka; Nenkova, Ruska; Ivanova, D.

doi:10.5504/bbeq.2013.0003

Cited by 15 publications

(5 citation statements)

References 38 publications

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“…The results indicated that the biotransformation of phenol, o-cresol, m-cresol, bromophenol, 2,4-DCP, 2CP, and 4CP by immobilized cultures was higher than in free cultures. Our findings are in agreement with Yordanova et al [33], who found that the degradation of phenol and 2,4-DCP by immobilized cultures was faster than 2-CP and 4-CP degradation. In addition, numerous reports demonstrated that the immobilized cultures process the highest removal potency for different CPs than free cultures [34][35][36].…”

Section: Discussionsupporting

confidence: 94%

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

Farag

El-Naggar

Ghanem

2022

Journal of Genetic Engineering and Biotechnology

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Background 2,4-Dichlorophenol (2,4-DCP) is a very toxic aromatic compound for humans and the environment and is highly resistant to degradation. Therefore, it is necessary to develop efficient remediation and cost-effective approaches to this pollutant. Microbial enzymes such as laccases can degrade phenols, but limited information is known about immobilized bacterial laccase and their reuse. Methods Immobilization of marine halophilic Bacillus subtilis AAK cultures via entrapment and adsorption techniques and degradation of different phenolic compounds by immobilized cells were estimated. Partial purification and immobilization of laccase enzymes were carried out. In addition, the biodegradation of 2,4-DCP and others contaminated by wastewater was investigated. Results Immobilization of cells and partially purified laccase enzymes by adsorption into 3% alginate increased 2,4-DCP biotransformation compared with free cells and free enzymes. In addition, the reuse of both the immobilized culture and laccase enzymes was evaluated. The highest removal of 2,4-DCP from pulp and paper wastewater samples inoculated by immobilized cells and the immobilized enzyme was 90% and 95%, respectively, at 50 h and 52 h of incubation, compared to free cells and free enzyme. Conclusion The results of this study have revealed the immobilization of a biocatalyst and its laccase enzyme as a promising technique for enhancing the degradation of 2,4-DCP and other toxic phenolic and aromatic compounds. The reuse of the biocatalyst and its laccase enzyme enabled the application of this cost-effective bioremediation strategy.

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Section: Discussionsupporting

confidence: 94%

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

Farag

El-Naggar

Ghanem

2022

Journal of Genetic Engineering and Biotechnology

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“…Finally, bacterial cells were cultured in MSM containing 20 mg/L BPA with an inoculum size of 1.5 × 10 7 CFU/mL for 10 days (150 rpm shaking at 30°C in a dark condition). In the primary screening, the residual of BPA in the supernatants was determined using the Folin–Ciocalteu (F-C) method ( 21 ). For the assay, 50 μL of the supernatants were mixed with 25 μL of F-C reagent in a 96-well plate and incubated at room temperature for 4 min.…”

Section: Methodsmentioning

confidence: 99%

Isolation and characterization of a bisphenol A-degrading strain, Pseudomonas aeruginosa DU2, from soil containing decaying plants

Chamak,

Farrokh,

Rostami

et al. 2023

IJM

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Background and Objectives: Bisphenol A (BPA) is a toxic compound with broad applications in the plastics industry. BPA has harmful effects on various organisms and its efficient removal is necessary. The microbial degradation of BPA is a safe and economical approach. In this research, soil samples containing decaying plants were screened to isolate a BPA-degrad- able bacterial strain. Materials and Methods: Soil samples were collected from different locations in Damghan, Semnan province, Iran. To enrich BPA-degrading bacteria, the samples were cultured in a stepwise manner in a mineral medium containing increasing BPA concentrations (5 to 40 mg/L). The ability of isolated bacteria in degrading BPA was assayed by Folin-Ciocalteu and high-performance liquid chromatography methods. The biodegradation efficiency of the most efficient isolate was assayed under distinct conditions and it was identified through the sequencing of the 16S rRNA gene. Results: Among the isolated bacteria, Pseudomonas aeruginosa DU2 (GenBank accession number: OP919484) showed the most BPA biodegradation ability. The highest BPA degradation (52.98%) was observed in the mineral medium containing 5 mg/L BPA and the inoculum size of 6 × 107 CFU/mL at pH 9 and in the presence of 0.05% (w/v) NaCl during 10 days. Conclusion: These results offer soil containing decaying plants as a promising source for finding BPA-degrading bacteria. P. aeruginosa DU2 has basal BPA removal ability, which could be improved by optimization of medium components and growth conditions.

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“…The percentage removal of BPA was determined using Folin-Ciocalteu reagent according to the method of Yordanova et al [37]. The residual of BPA supplemented in the mineral salt media was determined at 24-h intervals throughout the degradation period.…”

Section: Determination Of Bpa Degradationmentioning

confidence: 99%

“…The mixture was left for 5 min, and 2 mL of 20% Na 2 CO 3 (w/v) was added to the mixture. The solution was kept in the dark for 60 min, and therefore, absorbance at 750 nm was measured [37]. The degradation rate was expressed as the difference between the initial and final absorbance.…”

Section: Determination Of Bpa Degradationmentioning

confidence: 99%

A Laboratory-Scale Study: Biodegradation of Bisphenol A (BPA) by Different Actinobacterial Consortium

Adesanya¹,

Adesanya²

2022

Actinobacteria - Diversity, Applications and Medical Aspects

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The unique diversity of microbes makes them ideal for biotechnological purposes. In this present study, 16 actinobacterial isolates were screened on media supplemented with Bisphenol A (BPA). Three out of 16 isolates exhibited high biocapacity to degrade BPA as a carbon source. Four different mixed actinobacterial consortia were developed using the above strains and the effect of each consortium on biomass growth; laccase production and BPA degradation were examined. At 100-mg/L BPA concentration, the three-member consortium grew well with maximum laccase activity as well as maximal degradation rate of Bisphenol A than the other two-member consortium. The consortium of Actinomyces naeslundii, Actinomyces bovis, and Actinomyces israelii degraded 93.1% with maximum laccase activity of 15.9 U/mL, followed by A. naeslundii and A. israelii with 87.3% and 9.5 U/mL. This was followed by A. naeslundi and A. bovis with 80.4% and 8.7 U/mL, while A. bovis and A. israelii degraded 76.0% with laccase activity of 7.0. The gas chromatography–mass spectrometry (GC–MS) analysis of biodegraded BPA showed the presence of oxalic acid and new products like 1,2,4-trimethylbenzene and 2,9-dimethyldecane.

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Biodegradation of Phenol and Phenolic Derivatives by a Mixture of Immobilized Cells ofAspergillus AwamoriandTrichosporon Cutaneum

Cited by 15 publications

References 38 publications

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

2,4-Dichlorophenol biotransformation using immobilized marine halophilic Bacillus subtilis culture and laccase enzyme: application in wastewater treatment

Isolation and characterization of a bisphenol A-degrading strain, Pseudomonas aeruginosa DU2, from soil containing decaying plants

A Laboratory-Scale Study: Biodegradation of Bisphenol A (BPA) by Different Actinobacterial Consortium

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