Performance of OraQuick Advance® Rapid HIV-1/2 Antibody Test for detection of antibodies in oral fluid and serum/plasma in HIV-1+ subjects carrying different HIV-1 subtypes and recombinant variants

Rapid Detection of HIV-1 and 2 Antibodies by using Oral Fluid: A Multicentric Pilot Study in India

“…The efficacy of these tests has been evaluated individually and also by comparing both the tests. [22][23][24] In the present study, the OraQuick test demonstrated high sensitivity and specificity of 100% with oral fluid specimens as claimed by the manufacturer. These findings are in accordance with a previous study done in rural population in India, 25 although there were conflicting reports of lower sensitivity and specificity of oral fluid-based tests elsewhere with high incidence of false-positive and false-negative results.…”

“…31,32 There is a more recent study on this oral fluid-based test kit which demonstrated its utility for detecting infections due to HIV-1 subtypes and recombinants. 22 Although the present study demonstrated the usefulness of the oral fluid-based OraQuick ® rapid HIV-1 and 2 antibody test, the disadvantage lies in its inability to specify whether the sample contained HIV-1 or HIV-2 antibodies specifically, HIV-2 being less prevalent than HIV-1.…”

“…19,25 This can be used in areas with limited laboratory resources and they provide convenience in testing on site results by allowing the delivery of definite negative or provisional reactive results greatly enhancing interventional programs. 10,12,14,[21][22][23] There are rapid HIV tests using oral fluid and whole blood. The efficacy of these tests has been evaluated individually and also by comparing both the tests.…”

Community-Based Rapid Oral Human Immunodeficiency Virus Testing for Tuberculosis Patients in Lima, Peru

“…and specificity of 98.3-100%, and has proven its utility in detecting all HIV subtypes and recombinants common in developing countries. 41 OraQuick was chosen because it delivered results within 20 minutes, enabling CHWs to incorporate some HIV education and pre-test counseling, did not require refrigerated storage, and decreased biohazards related to using finger prick or needles.…”

HIV-1 Diversity and Its Implications in Diagnosis, Transmission, Disease Progression, and Antiretroviral Therapy

“…This kind of assay is simple, rapid, instrument-free and relatively cheap. However some of these assays have shown problems in detecting HIV-1 subtypes D, F, H, CRF02_AG, group O and HIV-2 (Aghokeng et al, 2009;Beelaert and Fransen, 2010;Chaillet et al, 2010;Holguin et al, 2009;Laforgerie et al, 2010;Pavie et al, 2010). Minor antigenic differences between isolates of different clades and the peptides/recombinant proteins used in these assays could explain the problems in the detection of some HIV genetic forms (Aghokeng et al, 2009;Laforgerie et al, 2010;Makuwa et al, 2002;Pavie et al, 2010).…”