Context: Version 5 of the Sport Concussion Assessment Tool (SCAT5) was released in 2017 with an additional 10-word list option in the memory section and additional instructions for completing the symptom scale.Objective: To provide reference scores for high school rugby union players on the SCAT5, including immediate memory using the 10-word list, and examine how age, sex, and concussion history affected performance.Design: Cross-sectional study. Setting: Calgary, Alberta high schools.Patients or Other Participants: High school rugby union players (ages 15-18 years) participating in a 2018 season cohort study (n ¼ 380, males ¼ 210, females ¼ 170).Main Outcome Measure(s): Sport Concussion Assessment Tool 5 scores, including total number of symptoms (of 20), symptom severity (of 132), 10-word immediate memory (of 30), delayed memory (of 10), modified Standardized Assessment of Concussion (of 50), and balance examination (of 30).Results: The median number of symptoms reported at baseline ranged from 5 to 8 across sex and age stratifications. Median symptom severity was lowest in males with no concussion history (7; range, 0-28) and highest in females with a concussion history (13, range ¼ 0-45). Median total scores on immediate memory were 2-3 (range ¼ 0-4) for males and 21 (range ¼ 9-29) for females. Median total scores were 3 (range ¼ 0-4) on digits backward and 7 (range ¼ 0-20) on delayed memory (all groups). Based on simultaneous quantile (q) regression at 0.50 and 0.75, adjusted for age and concussion history, being female was associated with a higher total symptoms score (q0.75 b female ¼ 2.85; 99% confidence interval [CI] ¼ 0.33, 5.37), higher total symptom severity score (q0.75 b female ¼ 8.00; 99% CI ¼ 2.83, 13.17), and lower number of errors on the balance examination (q0.75 b female ¼ À3.00; 99% CI ¼ À4.85, À1.15). Age and concussion history were not associated with any summary measures.Conclusions: The 10-word list option in the memory section reduced the likelihood of a ceiling effect. A player's sex may be an important consideration when interpreting the SCAT5 after concussion.Despite changes to the instructions for the baseline symptoms assessment, the majority of high school rugby players reported symptoms at baseline. Addition of the 10-word list to version 5 of the Sport Concussion Assessment Tool eliminated a ceiling effect for memory scores in high school rugby players. This study informs the use and interpretation of the Sport Concussion Assessment Tool, version 5, in Alberta high school rugby players and indicates that reporting of symptoms is normal at baseline in this age group.