Performance of an ethoxyethylacrylate stationary phase for open-tubular liquid chromatography

Abstract

Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractFused-silica capillaries of I.D. ca 11.4 tzm were coated with polyacrylate layers. The layers consisted of copolymers of silicone acrylate and ethoxyethylacrylate which were polymerized in situ by UV irradiation. Capillaries with phase ratios up to 0.9 were prepared. This results in very high mass loadabilities so that solute amounts suitable for UV detection can be injected without overloading the stationary phase. This is illustrated by the separation of various mixtures containing phenolic and aromatic compounds. The retention behaviour and the kinetic performance were studied with both UV and laser-induced fluorescence detection. On a 5-m column more than 10 6 plates could be generated for anthracene. Further, the possibility of on-column preconcentration and the effect of temperature on the kinetic performance were investigated on this stationary phase.

A weak cation-exchange phase for the separation of biogenic amines by open tubular liquid chromatography

“…The photopolymerization of acrylates to immobilize a polymer film in fused silica capillaries has been described before [7]. Briefly the procedure consists of the following successive steps:…”

“…capillaries with sufficient sample capacity necessary to avoid column overloading. The last couple of years we reported on the results of an in situ photopolymerization technique to create polymeric acrylate stationary phases in fused silica capillaries [3,[7][8][9]. This technique is different from other coating techniques in that the polymer is formed inside the capillary.…”

“…Various acrylic monomers e.g. with alkyl chains [3], an ethoxy group [7] and a hydroxy group [9] have been used. These stationary phases exhibited excellent separation performance in both reversed-and normal phase chromatography but had one major limitation: only neutral compounds could be retained.…”

“…capillaries in the past [3,[7][8][9], in this study we used laser induced fluorescence (LIF) detection to increase the detectability of the solutes. Native fluorescence of the biogenic amines was measured after excitation with 257 nm radiation from a frequency doubled argon ion laser.…”

Highly efficient separations on 5 μm internal diameter open tubular capillaries coated with thick polyacrylate stationary phases

“…fused silica capillaries by using in situ photopolymerization of acrylates [1,2]. Of the various stationary phases COnsidered that prepared from a mixture of siliconeand ethoxyethylacrylate (SiA-EEA) showed the best performance in terms of separation efficiency and coating success.…”