Experiences with a frequency-doubled argon ion laser for fluorescence detection in open tubular liquid chromatography

Swart, Remco; Elgersma, Jaap W.; Kraak, J.C.; Poppe, H.

doi:10.1002/(sici)1520-667x(1997)9:8<591::aid-mcs2>3.0.co;2-x

Cited by 7 publications

(4 citation statements)

References 15 publications

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“…A handy solution is to apply a thicker film of stationary phase, for which methods involving polyacrylate phases have recently been published. 10,11 Swelling of the stationary phase was earlier shown to be an easy and convenient way of increasing capacity and affinity without a severe loss of efficiency due to a more liquidlike stationary phase. 12,13 The preparation technique for open tubular columns with small inner diameters for LC use has been known for many years, 7 but there is still a lack of commercially available OTLC columns.…”

Section: The Concept Of Interfacing Open Tubular Liquid Chromatograph...mentioning

confidence: 99%

“…1-5 µm). [14][15][16] Such low flow rates entail compromising between efficiency and sensitivity 17 when microdetectors based on laser 10 and electrochemical techniques are used. 18 These detectors often demand derivatization to add the fluorophores, chromophores, or electrochemically active groups necessary for sensitive detection.…”

Section: The Concept Of Interfacing Open Tubular Liquid Chromatograph...mentioning

confidence: 99%

“…A problem with OTLC columns has been their small capacity factors, k ‘. A handy solution is to apply a thicker film of stationary phase, for which methods involving polyacrylate phases have recently been published. , Swelling of the stationary phase was earlier shown to be an easy and convenient way of increasing capacity and affinity without a severe loss of efficiency due to a more liquidlike stationary phase. , The preparation technique for open tubular columns with small inner diameters for LC use has been known for many years, but there is still a lack of commercially available OTLC columns. Despite the popular shift to open tubular GC columns during the last 10−20 years, a similar development within the LC field has been slow, largely due to the lack of good concentration-sensitive detectors.…”

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Coupling of Open Tubular Liquid Chromatography to Electrospray Mass Spectrometry with a Nanospray Interface

1999

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The concept of interfacing open tubular liquid chromatography (OTLC) to electrospray ionization mass spectroscopy (ESI-MS) is here introduced. This combination has the potential of becoming a powerful and generic tool for separation and quantification of various complex mixtures. We can demonstrate here how easy and straightforward it is to connect an open capillary LC column to nano-ESI, simply by drawing the column through a stainless steel tube onto which the high voltage (HV) is supplied. This method is compared with applying the HV to a gold-coated column tip and the standard dynamic nanospray interface supplied by the ESI-MS manufacturer. Reliability and stability are documented. OTLC-ESI-MS is demonstrated by separation of fatty acids in the negative mode.

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Section: The Concept Of Interfacing Open Tubular Liquid Chromatograph...mentioning

confidence: 99%

Section: The Concept Of Interfacing Open Tubular Liquid Chromatograph...mentioning

confidence: 99%

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confidence: 99%

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Coupling of Open Tubular Liquid Chromatography to Electrospray Mass Spectrometry with a Nanospray Interface

1999

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“…The emission light was measured with a photomultiplier tube (model PM 102 S, Macam, Livingston, Scotland) operated at -1000 V. The amplifier included a RC-filter with a time constant of 0.5 seconds. A detailed description of the UV LIF detection set up can be found elsewhere [17].…”

Section: Apparatusmentioning

confidence: 99%

A weak cation-exchange phase for the separation of biogenic amines by open tubular liquid chromatography

1997

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General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. SummaryThe preparation and performance of a weak cation-exchange stationary phase for Open Tubular Liquid Chromatography (OT-LC) was investigated. The stationary phase was prepared in 5.4 gm I.D. fused silica capillaries by in situ photopolymerization of a mixture of silicon acrylate and acrylic acid. The influence of pH, counter ion concentration and organic modifier concentration of the mobile phase on the retention was studied with catecholamines as test solutes using LIF detection. Other biological amines like amino acids, small peptides and nucleic acid derivatives could be separated on this stationary phase as well. The kinetic performance of the stationary phase was studied with several cations and neutral solutes.

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Determination of enantiomeric excess by capillary electrophoresis

Blomberg

Wan

2000

Electrophoresis

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Capillary electrophoresis (CE) is becoming an established method for the determination of chiral trace impurities. This paper provides an overview of the state of the art of CE for such determinations. Detection limits of 0.1% impurity is widely accepted as a minimum requirement for chiral trace impurity determinations. This can be relatively easily achieved with CE. However, determination of lower concentrations requires careful optimization of the separation system. Four factors that are of particular significance for trace enantiomeric determinations: resolution, limit of detection, linear range and type of detection, are discussed. Further, the advantages and disadvantages of derivatization in this context are treated as well as the separation approach, ie., direct chiral separation or separation after the formation of diastereomers. It is concluded that the limit of impurity detection can be about 0.05% when UV detection is employed. Using laser-induced fluorescence detection, a quantitative determination at the 0.005% level is often possible.

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Experiences with a frequency-doubled argon ion laser for fluorescence detection in open tubular liquid chromatography

Cited by 7 publications

References 15 publications

Coupling of Open Tubular Liquid Chromatography to Electrospray Mass Spectrometry with a Nanospray Interface

Coupling of Open Tubular Liquid Chromatography to Electrospray Mass Spectrometry with a Nanospray Interface

A weak cation-exchange phase for the separation of biogenic amines by open tubular liquid chromatography

Determination of enantiomeric excess by capillary electrophoresis

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