2015
DOI: 10.1017/s0022029915000084
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Performance of a real-time PCR assay in routine bovine mastitis diagnostics compared with in-depth conventional culture

Abstract: Reliable identification of the aetiological agent is crucial in mastitis diagnostics. Real-time PCR is a fast, automated tool for detecting the most common udder pathogens directly from milk. In this study aseptically taken quarter milk samples were analysed with a real-time PCR assay (Thermo Scientific PathoProof Mastitis Complete-12 Kit, Thermo Fisher Scientific Ltd.) and by semi-quantitative, in-depth bacteriological culture (BC). The aim of the study was to evaluate the diagnostic performance of the real-t… Show more

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Cited by 36 publications
(33 citation statements)
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References 26 publications
(40 reference statements)
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“…The samples with DNA of some target species were largely easy to interpret because in 49.0% of the samples DNA of only one target species or group of species was detected. This agrees with previous reports where the same PCR test was used with smaller materials [24, 28]. In samples with DNA of two bacterial species (25.2%), a dominant species, being the likely causal agent of IMI, was found in 66.2% of the samples.…”
Section: Discussionsupporting
confidence: 91%
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“…The samples with DNA of some target species were largely easy to interpret because in 49.0% of the samples DNA of only one target species or group of species was detected. This agrees with previous reports where the same PCR test was used with smaller materials [24, 28]. In samples with DNA of two bacterial species (25.2%), a dominant species, being the likely causal agent of IMI, was found in 66.2% of the samples.…”
Section: Discussionsupporting
confidence: 91%
“…Thus, in 75% of all samples, the causal pathogen could be identified and the result was unequivocal. These results can be considered diagnostically valid [24]. In 13.4% of the samples, DNA of more than two bacterial species was detected, up to ten species in one sample.…”
Section: Discussionmentioning
confidence: 95%
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“…Em relação ao diagnóstico dos agentes causadores de mastites, ao nível internacional existe uma tendência crescente de utilização de ferramentas moleculares para o diagnóstico de patógenos, complementando, assim, os resultados obtidos na microbiologia clássica (McDonald et al 2005, Cremonesi et al 2006, Yamagishi et al 2007, Santos et al 2008, Onni et al 2010, Shome et al 2011, Ajitkumar et al 2012, Keane et al 2013, Rovai et al 2014, Hiitiö et al 2015. No Brasil, a utilização das ferramentas moleculares no diagnóstico dos agentes causadores de mastite ainda é escassa; dos 22 trabalhos apresen-…”
Section: Utilização De Ferramentas Moleculares No Diagnóstico Da Mastiteunclassified