Performance, kinetics, and substrate utilization in a continuous yeast fermentation with cell recycle by ultrafiltration membranes

Damiano, Dominick; Shin, Cheung Soo; Ju, Nai-hu; Wang, Shaw S.

doi:10.1007/bf00252365

Cited by 23 publications

(6 citation statements)

References 31 publications

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“…High cell density cultures with different cell recycle fermentation systems have been studied by many investigators to improve ethanol productivity [1][2][3][4][5][6]. The best experimental results reported so far have been obtained from single-stage chemostat systems with a membrane module [6,7].…”

Section: Introductionmentioning

confidence: 80%

Very high ethanol productivity in an innovative continuous two-stage bioreactor with cell recycle

Chaabane

Aldiguier

Alfenore³

et al. 2006

Bioprocess Biosyst Eng

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The performance of an innovative two-stage continuous bioreactor with cell recycle-potentially capable of giving very high ethanol productivity-was investigated. The first stage was dedicated to cell growth, whereas the second stage was dedicated to ethanol production. A high cell density was obtained by an ultrafiltration module coupled to the outlet of the second reactor. A recycle loop from the second stage to the first one was tested to improve cell viability and activity. Cultivations of Saccharomyces cerevisiae in mineral medium on glucose were performed at 30 degrees Celsius and pH 4. At steady state, total biomass concentrations of 59 and 157 gDCW l(-1) and ethanol concentrations of 31 and 65 g l(-1) were obtained in the first and second stage, respectively. The residual glucose concentration was 73 g l(-1) in the first stage and close to zero in the second stage. The present study shows that a very high ethanol productivity (up to 41 g l(-1) h(-1)) can indeed be obtained with complete conversion of the glucose and with a high ethanol titre (8.3 degrees GL) in the two-stage system.

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Section: Introductionmentioning

confidence: 80%

Very high ethanol productivity in an innovative continuous two-stage bioreactor with cell recycle

Chaabane

Aldiguier

Alfenore³

et al. 2006

Bioprocess Biosyst Eng

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“…Multi-stage fermentor systems with cell recycling were observed to be advantageous compared to cell immobilization techniques due to improved diffusion and total recycling of microorganisms creating a homogenous fermentation broth (Tashiro et al, 2005). Hollow fiber recycle membranes for cell recycling has been shown to offer several advantages due to high filtration area and relatively high flux due to cross filtration, but are associated with disadvantages such as low strength compared to ceramic or steel filters and inability for steam sterilization (Damiano et al, 1985).…”

Section: Introductionmentioning

confidence: 98%

Effect of nutrient limitation and two-stage continuous fermentor design on productivities during “Clostridium ragsdalei” syngas fermentation

Kundiyana

Huhnke

Wilkins

2011

Bioresource Technology

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“…The concentration of yeast cells in a fermentor depends on the fermentor design and on the biomass recycle ratio (Damiano et al 1985), which also affects the ethanol concentration and overall ethanol productivity. In addition to the effective immobilization of cells in the fermentation system and maintenance of their metabolic activity, the membrane bioreactor enables reduction of the product inhibitory effect in consequence of its partial and continuous removal from the fermentation zone.…”

Section: Offprint Requests To: M Rychteramentioning

confidence: 99%

Application of a membrane recycle bioreactor for continuous ethanol production

Melzoch

Rychtera

Markvichov

et al. 1991

Appl Microbiol Biotechnol

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A series of continuous fermentations were carried out with a production strain of the yeast Saccharomyces cerevisiae in a membrane bioreactor. A membrane separation module composed of ultrafiltration tubular membranes retained all biomass in a fermentation zone of the bioreactor and allowed continuous removal of fermentation products into a cell-free permeate. In a system with total (100%) cell recycle the impact of fermentation conditions [dilution rate (0.03-0.3 h -l ) ; substrate concentration in the feed (50-300 g -l -l ) ; biomass concentration (depending on the experimental conditions)] was studied on the behaviour of the "immobilized" cell population and on ethanol formation.M a x i m u m ethanol productivity (15 g. 1-1. h -1) was attained at an ethanol concentration of 81 g-1 -1. The highest demands of cells for maintenance energy were found at the maximum feed substrate concentration (300 g-1-1) and at very low concentrations of cells in the broth.

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Performance, kinetics, and substrate utilization in a continuous yeast fermentation with cell recycle by ultrafiltration membranes

Cited by 23 publications

References 31 publications

Very high ethanol productivity in an innovative continuous two-stage bioreactor with cell recycle

Very high ethanol productivity in an innovative continuous two-stage bioreactor with cell recycle

Effect of nutrient limitation and two-stage continuous fermentor design on productivities during “Clostridium ragsdalei” syngas fermentation

Application of a membrane recycle bioreactor for continuous ethanol production

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